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F4JKB6

- PRRP3_ARATH

UniProt

F4JKB6 - PRRP3_ARATH

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Protein
Proteinaceous RNase P 3
Gene
PRORP3, At4g21900, T8O5.110
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Endonuclease RNase P responsible for the 5' maturation of tRNA precursors. Also involved in the maturation of mRNA and small nucleolar RNA (snoRNA).1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.1 Publication

Kineticsi

  1. KM=0.3 µM for tRNA(Gln) precursor1 Publication

GO - Molecular functioni

  1. ribonuclease P activity Source: UniProtKB

GO - Biological processi

  1. RNA phosphodiester bond hydrolysis Source: GOC
  2. RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
  3. mRNA processing Source: UniProtKB
  4. snoRNA processing Source: UniProtKB
  5. tRNA 5'-leader removal Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

tRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinaceous RNase P 3 (EC:3.1.26.5)
Gene namesi
Name:PRORP3
Ordered Locus Names:At4g21900
ORF Names:T8O5.110
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 4

Organism-specific databases

TAIRiAT4G21900.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype; due to the redundancy with PRORP2. Prorp2 and prorp3 double mutant is lethal.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi480 – 4812DD → AA: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 576576Proteinaceous RNase P 3
PRO_0000420274Add
BLAST

Proteomic databases

PRIDEiF4JKB6.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT4G21900.1-P.

Structurei

3D structure databases

ProteinModelPortaliF4JKB6.
SMRiF4JKB6. Positions 87-573.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati88 – 12336PPR 1
Add
BLAST
Repeati129 – 16638PPR 2
Add
BLAST
Repeati167 – 20135PPR 3
Add
BLAST
Repeati204 – 23835PPR 4
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi80 – 834Poly-Lys

Sequence similaritiesi

Belongs to the PPR family. P subfamily.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG68490.
HOGENOMiHOG000006117.
KOiK18213.
OMAiSCEPMAD.

Family and domain databases

InterProiIPR021869. RNase_Zc3h12.
[Graphical view]
PfamiPF11977. RNase_Zc3h12a. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4JKB6-1 [UniParc]FASTAAdd to Basket

« Hide

MKLKKPSLPS SLLCAVPPCL SQIRLLIPRR VRVSSSTFAN AKLVTLRNHT    50
VNLHIYYCSM AGTDNRRSRH DDESPKNPNK KKKGNRNPEK SLLINLHSCS 100
KRKDLSAALA LYDAAITSSD IRLNQQHFQS LLYLCSAFIS DPSLQTVAID 150
RGFQIFDRMV SSGISPNESS VTAVARLAAA KGDGDYAFKL VKDLVAVGGV 200
SVPRLRTYAP ALLCFCDTLE AEKGYEVEDH MDASGIVLEE AEISALLKVS 250
AATGRENKVY RYLQKLRECV GCVSEETSKA IEEWFYGVKA SEVSDNGIGS 300
DIELLRAAVL KNGGGWHGLG WVGEGKWIVK KGNVSSAGKC LSCDEHLACV 350
DTNEVETEDF VNSLVTLAME RKAKMNSCEP MADFSEFQEW LEKHGDYEAI 400
LDGANIGLYQ QNFADGGFSL PQLEAVVKEL YNKSGSKKQP LILLHKKRVN 450
ALLENPNHRN LVEEWINNNV LYATPPGSND DWYWLYAAAK LKCLLVTNDE 500
MRDHIFELLS NSFFQKWKER HQVRFTFVKG CLKLEMPPPF SVVIQESEKG 550
SWHVPITSQD KEESLRSWMC ITRQSS 576
Length:576
Mass (Da):64,396
Last modified:June 28, 2011 - v1
Checksum:i10A34B9C5F166DAD
GO

Sequence cautioni

The sequence BAF02238.1 differs from that shown. Reason: Intron retention.
The sequence BAE98777.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
The sequence CAA17157.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence CAB79145.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1851D → G in BAF02238. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL021890 Genomic DNA. Translation: CAA17157.1. Sequence problems.
AL161556 Genomic DNA. Translation: CAB79145.1. Sequence problems.
CP002687 Genomic DNA. Translation: AEE84521.1.
AK226669 mRNA. Translation: BAE98777.1. Different initiation.
AK230440 mRNA. Translation: BAF02238.1. Sequence problems.
PIRiT05472.
RefSeqiNP_193921.2. NM_118311.6.
UniGeneiAt.74070.

Genome annotation databases

EnsemblPlantsiAT4G21900.1; AT4G21900.1; AT4G21900.
GeneIDi828279.
KEGGiath:AT4G21900.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL021890 Genomic DNA. Translation: CAA17157.1 . Sequence problems.
AL161556 Genomic DNA. Translation: CAB79145.1 . Sequence problems.
CP002687 Genomic DNA. Translation: AEE84521.1 .
AK226669 mRNA. Translation: BAE98777.1 . Different initiation.
AK230440 mRNA. Translation: BAF02238.1 . Sequence problems.
PIRi T05472.
RefSeqi NP_193921.2. NM_118311.6.
UniGenei At.74070.

3D structure databases

ProteinModelPortali F4JKB6.
SMRi F4JKB6. Positions 87-573.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT4G21900.1-P.

Proteomic databases

PRIDEi F4JKB6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT4G21900.1 ; AT4G21900.1 ; AT4G21900 .
GeneIDi 828279.
KEGGi ath:AT4G21900.

Organism-specific databases

TAIRi AT4G21900.

Phylogenomic databases

eggNOGi NOG68490.
HOGENOMi HOG000006117.
KOi K18213.
OMAi SCEPMAD.

Family and domain databases

InterProi IPR021869. RNase_Zc3h12.
[Graphical view ]
Pfami PF11977. RNase_Zc3h12a. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
    Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B.
    , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
    Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Morosawa T., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K.
    , Akiyama K., Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  4. Cited for: IDENTIFICATION, SUBCELLULAR LOCATION.
  5. "PRORP proteins support RNase P activity in both organelles and the nucleus in Arabidopsis."
    Gutmann B., Gobert A., Giege P.
    Genes Dev. 26:1022-1027(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE, MUTAGENESIS OF 480-ASP-ASP-481, BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiPRRP3_ARATH
AccessioniPrimary (citable) accession number: F4JKB6
Secondary accession number(s): O49713, Q0WKW9, Q0WVS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: June 28, 2011
Last modified: July 9, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi