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Protein

Proteinaceous RNase P 3

Gene

PRORP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease RNase P responsible for the 5' maturation of tRNA precursors. Also involved in the maturation of mRNA and small nucleolar RNA (snoRNA).1 Publication

Catalytic activityi

Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 Mg2+ or Mg2+ ions per subunit.By similarity

Kineticsi

  1. KM=0.3 µM for tRNA(Gln) precursor1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi340ZincBy similarity1
    Metal bindingi343ZincBy similarity1
    Metal bindingi402Magnesium or manganese 1; catalyticBy similarity1
    Metal bindingi480Magnesium or manganese 1; catalyticBy similarity1
    Metal bindingi481Magnesium or manganese 2; catalyticBy similarity1
    Metal bindingi499Magnesium or manganese 2; catalyticBy similarity1
    Metal bindingi553Zinc; via tele nitrogenBy similarity1
    Metal bindingi570ZincBy similarity1

    GO - Molecular functioni

    • metal ion binding Source: UniProtKB-KW
    • ribonuclease P activity Source: UniProtKB

    GO - Biological processi

    • mRNA processing Source: UniProtKB
    • snoRNA processing Source: UniProtKB
    • tRNA 5'-leader removal Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase, Nuclease
    Biological processtRNA processing
    LigandMagnesium, Manganese, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proteinaceous RNase P 3 (EC:3.1.26.5)
    Gene namesi
    Name:PRORP3
    Ordered Locus Names:At4g21900
    ORF Names:T8O5.110
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 4

    Organism-specific databases

    AraportiAT4G21900
    TAIRilocus:2141662 AT4G21900

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype; due to the redundancy with PRORP2. Prorp2 and prorp3 double mutant is lethal.1 Publication

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi480 – 481DD → AA: Loss of activity. 1 Publication2

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004202741 – 576Proteinaceous RNase P 3Add BLAST576

    Proteomic databases

    PaxDbiF4JKB6
    PRIDEiF4JKB6

    Expressioni

    Gene expression databases

    ExpressionAtlasiF4JKB6 baseline and differential

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT4G21900.1

    Structurei

    3D structure databases

    ProteinModelPortaliF4JKB6
    SMRiF4JKB6
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Repeati88 – 123PPR 1Add BLAST36
    Repeati129 – 166PPR 2Add BLAST38
    Repeati167 – 201PPR 3Add BLAST35
    Repeati204 – 238PPR 4Add BLAST35
    Domaini335 – 570PRORPAdd BLAST236

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi80 – 83Poly-Lys4

    Sequence similaritiesi

    Belongs to the PPR family. P subfamily.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiKOG1347 Eukaryota
    COG0534 LUCA
    HOGENOMiHOG000006117
    InParanoidiF4JKB6
    KOiK18213
    OMAiTEASHGC
    OrthoDBiEOG093607Y2

    Family and domain databases

    Gene3Di1.25.40.10, 2 hits
    InterProiView protein in InterPro
    IPR033443 PPR_long
    IPR031595 PRORP_C
    IPR011990 TPR-like_helical_dom_sf
    PfamiView protein in Pfam
    PF17177 PPR_long, 1 hit
    PF16953 PRORP, 1 hit

    Sequencei

    Sequence statusi: Complete.

    F4JKB6-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKLKKPSLPS SLLCAVPPCL SQIRLLIPRR VRVSSSTFAN AKLVTLRNHT
    60 70 80 90 100
    VNLHIYYCSM AGTDNRRSRH DDESPKNPNK KKKGNRNPEK SLLINLHSCS
    110 120 130 140 150
    KRKDLSAALA LYDAAITSSD IRLNQQHFQS LLYLCSAFIS DPSLQTVAID
    160 170 180 190 200
    RGFQIFDRMV SSGISPNESS VTAVARLAAA KGDGDYAFKL VKDLVAVGGV
    210 220 230 240 250
    SVPRLRTYAP ALLCFCDTLE AEKGYEVEDH MDASGIVLEE AEISALLKVS
    260 270 280 290 300
    AATGRENKVY RYLQKLRECV GCVSEETSKA IEEWFYGVKA SEVSDNGIGS
    310 320 330 340 350
    DIELLRAAVL KNGGGWHGLG WVGEGKWIVK KGNVSSAGKC LSCDEHLACV
    360 370 380 390 400
    DTNEVETEDF VNSLVTLAME RKAKMNSCEP MADFSEFQEW LEKHGDYEAI
    410 420 430 440 450
    LDGANIGLYQ QNFADGGFSL PQLEAVVKEL YNKSGSKKQP LILLHKKRVN
    460 470 480 490 500
    ALLENPNHRN LVEEWINNNV LYATPPGSND DWYWLYAAAK LKCLLVTNDE
    510 520 530 540 550
    MRDHIFELLS NSFFQKWKER HQVRFTFVKG CLKLEMPPPF SVVIQESEKG
    560 570
    SWHVPITSQD KEESLRSWMC ITRQSS
    Length:576
    Mass (Da):64,396
    Last modified:June 28, 2011 - v1
    Checksum:i10A34B9C5F166DAD
    GO

    Sequence cautioni

    The sequence BAE98777 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
    The sequence BAF02238 differs from that shown. Intron retention.Curated
    The sequence CAA17157 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence CAB79145 differs from that shown. Reason: Erroneous gene model prediction.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti185D → G in BAF02238 (Ref. 3) Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AL021890 Genomic DNA Translation: CAA17157.1 Sequence problems.
    AL161556 Genomic DNA Translation: CAB79145.1 Sequence problems.
    CP002687 Genomic DNA Translation: AEE84521.1
    AK226669 mRNA Translation: BAE98777.1 Different initiation.
    AK230440 mRNA Translation: BAF02238.1 Sequence problems.
    PIRiT05472
    RefSeqiNP_193921.2, NM_118311.7
    UniGeneiAt.74070

    Genome annotation databases

    EnsemblPlantsiAT4G21900.1; AT4G21900.1; AT4G21900
    GeneIDi828279
    GrameneiAT4G21900.1; AT4G21900.1; AT4G21900
    KEGGiath:AT4G21900

    Similar proteinsi

    Entry informationi

    Entry nameiPRRP3_ARATH
    AccessioniPrimary (citable) accession number: F4JKB6
    Secondary accession number(s): O49713, Q0WKW9, Q0WVS1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: June 28, 2011
    Last modified: May 23, 2018
    This is version 49 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

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