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Protein

Protein DEFECTIVE IN EXINE FORMATION 1

Gene

DEX1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for exine pattern formation during pollen development, especially for primexine deposition.1 Publication

GO - Molecular functioni

  • calcium ion binding Source: TAIR

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pollen exine formation Source: UniProtKB
  • pollen wall assembly Source: TAIR

Keywordsi

Biological processCell wall biogenesis/degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Protein DEFECTIVE IN EXINE FORMATION 11 Publication
Gene namesi
Name:DEX11 Publication
Ordered Locus Names:At3g09090Imported
ORF Names:MZB10.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G09090
TAIRilocus:2095274 AT3G09090

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 858ExtracellularSequence analysisAdd BLAST834
Transmembranei859 – 879HelicalSequence analysisAdd BLAST21
Topological domaini880 – 896CytoplasmicSequence analysisAdd BLAST17

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Male sterile. Defective pollen wall pattern formation at early tetrad stage; delayed and reduced primexine deposition, as well as abnormal rippling of the plasma membrane and no production of spacers. Random sporopollenin deposition on the plasma membrane along the microspore wall.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000043181025 – 896Protein DEFECTIVE IN EXINE FORMATION 1Sequence analysisAdd BLAST872

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi20N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi204N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi211N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi239N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi253N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi266N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi279N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi295N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi302N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi307N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi317N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi789N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiF4IYM4

Expressioni

Tissue specificityi

Mostly expressed in buds, and, to a lower extent, in leaves, roots and seedlings.1 Publication

Gene expression databases

ExpressionAtlasiF4IYM4 baseline and differential
GenevisibleiF4IYM4 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT3G09090.1

Structurei

3D structure databases

ProteinModelPortaliF4IYM4
SMRiF4IYM4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IEFE Eukaryota
ENOG410ZQCN LUCA
HOGENOMiHOG000030812
InParanoidiF4IYM4
OMAiAYRYNRE
OrthoDBiEOG0936021C

Family and domain databases

Gene3Di2.130.10.10, 1 hit
InterProiView protein in InterPro
IPR013517 FG-GAP
IPR015943 WD40/YVTN_repeat-like_dom_sf
PfamiView protein in Pfam
PF01839 FG-GAP, 1 hit

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: F4IYM4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKSRARQCLL VCLLCLSLTN LSYGENKFRE RKATDDELGY PDIDEDALLN
60 70 80 90 100
TQCPKKLELR WQTEVTSSVY ATPLIADINS DGKLDIVVPS FVHYLEVLEG
110 120 130 140 150
ADGDKMPGWP AFHQSNVHSS PLLFDIDKDG VREIALATYN AEVLFFRVSG
160 170 180 190 200
FLMSDKLEVP RRKVHKNWHV GLNPDPVDRS HPDVHDDVLE EEAMAMKSST
210 220 230 240 250
TQTNATTTTP NVTVSMTKEV HGANSYVSTQ EDQKRPENNQ TEAIVKPTPE
260 270 280 290 300
LHNSSMDAGA NNLAANATTA GSRENLNRNV TTNEVDQSKI SGDKNETVIK
310 320 330 340 350
LNTSTGNSSE TLGTSGNSST AETVTKSGRR LLEEDGSKES VDSHSDSKDN
360 370 380 390 400
SEGVRMATVE NDGGLEADAD SSFELLREND ELADEYSYDY DDYVDEKMWG
410 420 430 440 450
DEEWVEGQHE NSEDYVNIDA HILCTPVIAD IDKDGVQEMI VAVSYFFDPE
460 470 480 490 500
YYDNPEHLKE LGGIDIKNYI ASSIVVFNLD TKQVKWIKEL DLSTDKANFR
510 520 530 540 550
AYIYSSPTVV DLDGDGYLDI LVGTSFGLFY AMDHRGNIRE KFPLEMAEIQ
560 570 580 590 600
GAVVAADIND DGKIELVTTD SHGNIAAWTT QGVEIWEAHL KSLVPQGPSI
610 620 630 640 650
GDVDGDGHTE VVVPTSSGNI YVLSGKDGSI VRPYPYRTHG RVMNQLLLVD
660 670 680 690 700
LNKRGEKKKG LTIVTTSFDG YLYLIDGPTS CTDVVDIGET SYSMVLADNV
710 720 730 740 750
DGGDDLDLIV STMNGNVFCF STPSPHHPLK AWRSSDQGRN NKANRYDREG
760 770 780 790 800
VFVTHSTRGF RDEEGKNFWA EIEIVDKYRY PSGSQAPYNV TTTLLVPGNY
810 820 830 840 850
QGERRITQSQ IYDRPGKYRI KLPTVGVRTT GTVMVEMADK NGLHFSDEFS
860 870 880 890
LTFHMYYYKL LKWLLVLPML GMFGLLVILR PQEAVPLPSF SRNTDL
Length:896
Mass (Da):99,711
Last modified:June 28, 2011 - v1
Checksum:i027B36C638763C83
GO
Isoform 2 (identifier: F4IYM4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     791-894: TTTLLVPGNY...VPLPSFSRNT → TLFSNTDDAVGSRQLPGREEDNAEP

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:817
Mass (Da):90,462
Checksum:iF8F0E34A104A7274
GO
Isoform 3 (identifier: F4IYM4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     875-896: LLVILRPQEAVPLPSFSRNTDL → GTHPHRAHQKDPGTISC

Note: Derived from EST data. No experimental confirmation available.Imported
Show »
Length:891
Mass (Da):99,074
Checksum:i1E4DDEBC593395F2
GO

Sequence cautioni

The sequence AAD56325 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti229T → I in AAG31445 (PubMed:11743117).Curated1
Sequence conflicti367A → R in AAG31445 (PubMed:11743117).Curated1
Sequence conflicti367A → R in AAG31444 (PubMed:11743117).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_057386791 – 894TTTLL…FSRNT → TLFSNTDDAVGSRQLPGREE DNAEP in isoform 2. Add BLAST104
Alternative sequenceiVSP_057387875 – 896LLVIL…RNTDL → GTHPHRAHQKDPGTISC in isoform 3. Add BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF257186 Genomic DNA Translation: AAG31444.1
AF257187 mRNA Translation: AAG31445.1
AC009326 Genomic DNA Translation: AAD56325.1 Sequence problems.
CP002686 Genomic DNA Translation: AEE74719.1
CP002686 Genomic DNA Translation: AEE74720.1
CP002686 Genomic DNA Translation: AEE74721.1
BT005785 mRNA Translation: AAO64188.1
AK228583 mRNA Translation: BAF00499.1
RefSeqiNP_001118602.1, NM_001125130.1 [F4IYM4-2]
NP_001189844.1, NM_001202915.1 [F4IYM4-3]
NP_566343.1, NM_111744.5 [F4IYM4-1]
UniGeneiAt.16907
At.69661

Genome annotation databases

EnsemblPlantsiAT3G09090.1; AT3G09090.1; AT3G09090 [F4IYM4-1]
AT3G09090.2; AT3G09090.2; AT3G09090 [F4IYM4-2]
AT3G09090.3; AT3G09090.3; AT3G09090 [F4IYM4-3]
GeneIDi820063
GrameneiAT3G09090.1; AT3G09090.1; AT3G09090 [F4IYM4-1]
AT3G09090.2; AT3G09090.2; AT3G09090 [F4IYM4-2]
AT3G09090.3; AT3G09090.3; AT3G09090 [F4IYM4-3]
KEGGiath:AT3G09090

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDEX1_ARATH
AccessioniPrimary (citable) accession number: F4IYM4
Secondary accession number(s): B3H6A9
, F4IYM3, Q84TH7, Q9FV29, Q9FV30, Q9SS78
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 4, 2015
Last sequence update: June 28, 2011
Last modified: April 25, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health