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Protein

Thiamine pyrophosphokinase 2

Gene

TPK2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of thiamine to thiamine pyrophosphate (TPP). TPP is an active cofactor for enzymes involved in glycolysis and energy production. Plant leaves require high levels of TPP for photosynthesis and carbohydrate metabolism.1 Publication

Catalytic activityi

ATP + thiamine = AMP + thiamine diphosphate.

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes thiamine diphosphate from thiamine.
Proteins known to be involved in this subpathway in this organism are:
  1. Thiamine pyrophosphokinase 2 (TPK2), Thiamine pyrophosphokinase (AXX17_At1g02080), Thiamine pyrophosphokinase (TPK1), Thiamine pyrophosphokinase (AXX17_At2g42340), Thiamine pyrophosphokinase 1 (TPK1), Thiamine pyrophosphokinase (AXX17_At2g42340)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes thiamine diphosphate from thiamine, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: UniProtKB-KW
  • thiamine binding Source: InterPro
  • thiamine diphosphokinase activity Source: TAIR

GO - Biological processi

  • thiamine diphosphate biosynthetic process Source: GO_Central
  • thiamine metabolic process Source: TAIR

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.6.2. 399.
ReactomeiR-ATH-196819. Vitamin B1 (thiamin) metabolism.
UniPathwayiUPA00060; UER00597.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine pyrophosphokinase 2 (EC:2.7.6.2)
Short name:
AtTPK2
Alternative name(s):
Thiamine kinase 2
Gene namesi
Name:TPK2
Ordered Locus Names:At2g44750
ORF Names:F16B22.24
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G44750.
TAIRilocus:2042436. AT2G44750.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions. Tpk1 and tpk2 double mutants exhibit a seedling lethal phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004239671 – 267Thiamine pyrophosphokinase 2Add BLAST267

Proteomic databases

PaxDbiF4IV16.

Expressioni

Tissue specificityi

Expressed in leaves and at lower levels in flowers.1 Publication

Gene expression databases

GenevisibleiF4IV16. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G44750.2.

Structurei

3D structure databases

ProteinModelPortaliF4IV16.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiamine pyrophosphokinase family.Curated

Phylogenomic databases

eggNOGiKOG3153. Eukaryota.
COG1564. LUCA.
HOGENOMiHOG000180834.
KOiK00949.
OMAiTGNLKYC.
OrthoDBiEOG09360JCF.
PhylomeDBiF4IV16.

Family and domain databases

CDDicd07995. TPK. 1 hit.
Gene3Di2.60.120.320. 1 hit.
3.40.50.10240. 1 hit.
InterProiView protein in InterPro
IPR006282. Thi_PPkinase.
IPR016966. Thiamin_pyrophosphokinase_euk.
IPR007373. Thiamin_PyroPKinase_B1-bd.
IPR036371. TPK_B1-bd_sf.
IPR007371. TPK_catalytic.
IPR036759. TPK_catalytic_sf.
PfamiView protein in Pfam
PF04265. TPK_B1_binding. 1 hit.
PF04263. TPK_catalytic. 1 hit.
PIRSFiPIRSF031057. Thiamin_pyrophosphokinase. 1 hit.
SMARTiView protein in SMART
SM00983. TPK_B1_binding. 1 hit.
SUPFAMiSSF63862. SSF63862. 1 hit.
SSF63999. SSF63999. 1 hit.
TIGRFAMsiTIGR01378. thi_PPkinase. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: F4IV16-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLSAMDVMIH SSSFLLPCDE TCGTRYALVV LNQNLPRFTP LLWEHAKLRL
60 70 80 90 100
CADGGANRIY DELPLFFPHE DPFVIRNRYK PDVIKGDMDS IRRDVLDFYV
110 120 130 140 150
YWGTKVIDES HDQDTTDLDK CISYIRHSTL NQESSRLQIL ATGALGGRFD
160 170 180 190 200
HEAGNLNVLY RYPDTRIVLL SDDCLIQLLP KTHRHEIHIH SSLQGPHCGL
210 220 230 240 250
IPIGTPSANT TTSGLKWDLS NTEMRFGGLI STSNLVKEEI ITVESDSDLL
260
WTISIKKTGL PVQDHKP
Length:267
Mass (Da):30,214
Last modified:June 28, 2011 - v1
Checksum:i4EB8B19CE2F12699
GO
Isoform 2 (identifier: F4IV16-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     137-138: Missing.

Show »
Length:265
Mass (Da):29,973
Checksum:iDC19226CBFAF12BC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_053287137 – 138Missing in isoform 2. 1 Publication2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003672 Genomic DNA. Translation: AAC27477.2.
CP002685 Genomic DNA. Translation: AEC10463.1.
CP002685 Genomic DNA. Translation: AEC10464.1.
AY042898 mRNA. Translation: AAK68838.1.
AY081493 mRNA. Translation: AAM10055.1.
PIRiT01602.
RefSeqiNP_566026.1. NM_130040.3. [F4IV16-2]
NP_850424.1. NM_180093.2. [F4IV16-1]
UniGeneiAt.48590.
At.70253.

Genome annotation databases

EnsemblPlantsiAT2G44750.1; AT2G44750.1; AT2G44750. [F4IV16-2]
AT2G44750.2; AT2G44750.2; AT2G44750. [F4IV16-1]
GeneIDi819084.
GrameneiAT2G44750.1; AT2G44750.1; AT2G44750.
AT2G44750.2; AT2G44750.2; AT2G44750.
KEGGiath:AT2G44750.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiTPK2_ARATH
AccessioniPrimary (citable) accession number: F4IV16
Secondary accession number(s): O80514, Q94B25
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: June 28, 2011
Last modified: October 25, 2017
This is version 50 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families