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F4IAG2

- SSY3_ARATH

UniProt

F4IAG2 - SSY3_ARATH

Protein

Starch synthase 3, chloroplastic/amyloplastic

Gene

SS3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Involved in the synthesis of glycan chains within amylopectin in leaves. May play a regulatory role in the control of starch accumulation in plastids.3 Publications

    Catalytic activityi

    ADP-glucose + (1,4-alpha-D-glucosyl)(n) = ADP + (1,4-alpha-D-glucosyl)(n+1).

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei608 – 6081ADP-glucoseBy similarity

    GO - Molecular functioni

    1. identical protein binding Source: IntAct
    2. starch binding Source: InterPro
    3. starch synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. amylopectin biosynthetic process Source: UniProtKB
    2. starch biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Starch biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00152.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Starch synthase 3, chloroplastic/amyloplastic (EC:2.4.1.21)
    Short name:
    AtSS3
    Alternative name(s):
    Soluble starch synthase III
    Gene namesi
    Name:SS3
    Ordered Locus Names:At1g11720
    ORF Names:F25C20.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G11720.

    Subcellular locationi

    GO - Cellular componenti

    1. amyloplast Source: UniProtKB-SubCell
    2. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Amyloplast, Chloroplast, Plastid

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but mutant plants accumulate increased levels of starch and have starch granules with alterated morphology.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4444ChloroplastSequence AnalysisAdd
    BLAST
    Chaini45 – 1042998Starch synthase 3, chloroplastic/amyloplasticPRO_0000419770Add
    BLAST

    Proteomic databases

    PRIDEiF4IAG2.

    Expressioni

    Tissue specificityi

    Expressed in leaves and flowers.1 Publication

    Gene expression databases

    ArrayExpressiF4IAG2.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself4EBI-7661720,EBI-7661720

    Protein-protein interaction databases

    MINTiMINT-7299396.
    STRINGi3702.AT1G11720.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliF4IAG2.
    SMRiF4IAG2. Positions 594-1038.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili247 – 30256Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Coiled coil, Transit peptide

    Family and domain databases

    HAMAPiMF_00484. Glycogen_synth.
    InterProiIPR005085. CBM_fam25.
    IPR001296. Glyco_trans_1.
    IPR011835. Glycogen/starch_synth.
    IPR013534. Starch_synth_cat_dom.
    [Graphical view]
    PfamiPF03423. CBM_25. 3 hits.
    PF08323. Glyco_transf_5. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view]
    SMARTiSM01066. CBM_25. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: F4IAG2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MISYFLNQDF SRKKQGRMAA SGPKSSGPRG FGRRTTVGSA QKRTQKKNGE     50
    KDSNATSTAT NEVSGISKLP AAKVDVQKQS SVVLNERNVL DRSDIEDGSD 100
    RLDKKTTDDD DLLEQKLKLE RENLRRKEIE TLAAENLARG DRMFVYPVIV 150
    KPDEDIEVFL NRNLSTLNNE PDVLIMGAFN EWRWKSFTRR LEKTWIHEDW 200
    LSCLLHIPKE AYKMDFVFFN GQSVYDNNDS KDFCVEIKGG MDKVDFENFL 250
    LEEKLREQEK LAKEEAERER QKEEKRRIEA QKAAIEADRA QAKAETQKRR 300
    ELLQPAIKKA VVSAENVWYI EPSDFKAEDT VKLYYNKRSG PLTNSKELWL 350
    HGGFNNWVDG LSIVVKLVNA ELKDVDPKSG NWWFAEVVVP GGALVIDWVF 400
    ADGPPKGAFL YDNNGYQDFH ALVPQKLPEE LYWLEEENMI FRKLQEDRRL 450
    KEEVMRAKME KTARLKAETK ERTLKKFLLS QKDVVYTEPL EIQAGNPVTV 500
    LYNPANTVLN GKPEVWFRGS FNRWTHRLGP LPPQKMEATD DESSHVKTTA 550
    KVPLDAYMMD FVFSEKEDGG IFDNKNGLDY HLPVVGGISK EPPLHIVHIA 600
    VEMAPIAKVG GLGDVVTSLS RAVQELNHNV DIVFPKYDCI KHNFVKDLQF 650
    NRSYHWGGTE IKVWHGKVEG LSVYFLDPQN GLFQRGCVYG CADDAGRFGF 700
    FCHAALEFLL QGGFHPDILH CHDWSSAPVS WLFKDHYTQY GLIKTRIVFT 750
    IHNLEFGANA IGKAMTFADK ATTVSPTYAK EVAGNSVISA HLYKFHGIIN 800
    GIDPDIWDPY NDNFIPVPYT SENVVEGKRA AKEELQNRLG LKSADFPVVG 850
    IITRLTHQKG IHLIKHAIWR TLERNGQVVL LGSAPDPRIQ NDFVNLANQL 900
    HSSHGDRARL VLTYDEPLSH LIYAGADFIL VPSIFEPCGL TQLIAMRYGA 950
    VPVVRKTGGL FDTVFDVDHD KERAQAQVLE PNGFSFDGAD APGVDYALNR 1000
    AISAWYDGRE WFNSLCKTVM EQDWSWNRPA LEYLELYHSA RK 1042
    Length:1,042
    Mass (Da):118,512
    Last modified:June 28, 2011 - v1
    Checksum:iC0481F694FCDC7D2
    GO

    Sequence cautioni

    The sequence AAD30251.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti741 – 7411G → D in ABB46391. (PubMed:15908598)Curated
    Sequence conflicti1041 – 10411R → C in ABB46391. (PubMed:15908598)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007296 Genomic DNA. Translation: AAD30251.1. Different initiation.
    CP002684 Genomic DNA. Translation: AEE28774.1.
    DQ241810 mRNA. Translation: ABB46391.1.
    EF636491 mRNA. Translation: ABU96740.1.
    DQ415727 mRNA. Translation: ABD77100.1.
    PIRiH86250.
    RefSeqiNP_172637.2. NM_101044.3. [F4IAG2-1]
    UniGeneiAt.42115.

    Genome annotation databases

    EnsemblPlantsiAT1G11720.1; AT1G11720.1; AT1G11720. [F4IAG2-1]
    GeneIDi837716.
    KEGGiath:AT1G11720.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC007296 Genomic DNA. Translation: AAD30251.1 . Different initiation.
    CP002684 Genomic DNA. Translation: AEE28774.1 .
    DQ241810 mRNA. Translation: ABB46391.1 .
    EF636491 mRNA. Translation: ABU96740.1 .
    DQ415727 mRNA. Translation: ABD77100.1 .
    PIRi H86250.
    RefSeqi NP_172637.2. NM_101044.3. [F4IAG2-1 ]
    UniGenei At.42115.

    3D structure databases

    ProteinModelPortali F4IAG2.
    SMRi F4IAG2. Positions 594-1038.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-7299396.
    STRINGi 3702.AT1G11720.1-P.

    Proteomic databases

    PRIDEi F4IAG2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G11720.1 ; AT1G11720.1 ; AT1G11720 . [F4IAG2-1 ]
    GeneIDi 837716.
    KEGGi ath:AT1G11720.

    Organism-specific databases

    TAIRi AT1G11720.

    Enzyme and pathway databases

    UniPathwayi UPA00152 .

    Gene expression databases

    ArrayExpressi F4IAG2.

    Family and domain databases

    HAMAPi MF_00484. Glycogen_synth.
    InterProi IPR005085. CBM_fam25.
    IPR001296. Glyco_trans_1.
    IPR011835. Glycogen/starch_synth.
    IPR013534. Starch_synth_cat_dom.
    [Graphical view ]
    Pfami PF03423. CBM_25. 3 hits.
    PF08323. Glyco_transf_5. 1 hit.
    PF00534. Glycos_transf_1. 1 hit.
    [Graphical view ]
    SMARTi SM01066. CBM_25. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Mutations affecting starch synthase III in Arabidopsis alter leaf starch structure and increase the rate of starch synthesis."
      Zhang X., Myers A.M., James M.G.
      Plant Physiol. 138:663-674(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-1042, FUNCTION, DISRUPTION PHENOTYPE.
    4. "Role of the N-terminal starch-binding domains in the kinetic properties of starch synthase III from Arabidopsis thaliana."
      Valdez H.A., Busi M.V., Wayllace N.Z., Parisi G., Ugalde R.A., Gomez-Casati D.F.
      Biochemistry 47:3026-3032(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-1042 AND 595-1042.
      Strain: cv. Columbia.
    5. "The phenotype of soluble starch synthase IV defective mutants of Arabidopsis thaliana suggests a novel function of elongation enzymes in the control of starch granule formation."
      Roldan I., Wattebled F., Mercedes Lucas M., Delvalle D., Planchot V., Jimenez S., Perez R., Ball S., D'Hulst C., Merida A.
      Plant J. 49:492-504(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, DISRUPTION PHENOTYPE.
      Strain: cv. Columbia.
    6. "Overlapping functions of the starch synthases SSII and SSIII in amylopectin biosynthesis in Arabidopsis."
      Zhang X., Szydlowski N., Delvalle D., D'Hulst C., James M.G., Myers A.M.
      BMC Plant Biol. 8:96-96(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: cv. Columbia.
    7. "Starch granule initiation in Arabidopsis requires the presence of either class IV or class III starch synthases."
      Szydlowski N., Ragel P., Raynaud S., Lucas M.M., Roldan I., Montero M., Munoz F.J., Ovecka M., Bahaji A., Planchot V., Pozueta-Romero J., D'Hulst C., Merida A.
      Plant Cell 21:2443-2457(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
      Strain: cv. Wassilewskija.

    Entry informationi

    Entry nameiSSY3_ARATH
    AccessioniPrimary (citable) accession number: F4IAG2
    Secondary accession number(s): Q1WAB7, Q306T0, Q9SAA5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 31, 2012
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 29 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3