ID   F4I9T8_ARATH            Unreviewed;       495 AA.
AC   F4I9T8;
DT   28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT   28-JUN-2011, sequence version 1.
DT   24-JAN-2024, entry version 66.
DE   SubName: Full=4-coumarate:CoA ligase 3 {ECO:0000313|EMBL:AEE34324.1};
GN   Name=4CL3 {ECO:0000313|EMBL:AEE34324.1, ECO:0000313|TAIR:AT1G65060};
GN   Synonyms=4-COUMARATE:COA LIGASE {ECO:0000313|EMBL:AEE34324.1},
GN   4-coumarate:CoA ligase 3 {ECO:0000313|EMBL:AEE34324.1};
GN   OrderedLocusNames=At1g65060 {ECO:0000313|Araport:AT1G65060,
GN   ECO:0000313|EMBL:AEE34324.1};
GN   ORFNames=F16G16.6 {ECO:0000313|EMBL:AEE34324.1}, F16G16_6
GN   {ECO:0000313|EMBL:AEE34324.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000313|EMBL:AEE34324.1, ECO:0000313|Proteomes:UP000006548};
RN   [1] {ECO:0000313|EMBL:AEE34324.1, ECO:0000313|Proteomes:UP000006548}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2] {ECO:0000313|Proteomes:UP000006548}
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia {ECO:0000313|Proteomes:UP000006548};
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
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DR   EMBL; CP002684; AEE34324.1; -; Genomic_DNA.
DR   RefSeq; NP_849844.1; NM_179513.2.
DR   AlphaFoldDB; F4I9T8; -.
DR   SMR; F4I9T8; -.
DR   ProteomicsDB; 210190; -.
DR   EnsemblPlants; AT1G65060.2; AT1G65060.2; AT1G65060.
DR   GeneID; 842814; -.
DR   Gramene; AT1G65060.2; AT1G65060.2; AT1G65060.
DR   Araport; AT1G65060; -.
DR   TAIR; AT1G65060; 4CL3.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; F4I9T8; baseline and differential.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016874; F:ligase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.12780; N-terminal domain of ligase-like; 1.
DR   InterPro; IPR020845; AMP-binding_CS.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig_com.
DR   InterPro; IPR042099; ANL_N_sf.
DR   PANTHER; PTHR24096:SF169; 4-COUMARATE--COA LIGASE 3; 1.
DR   PANTHER; PTHR24096; LONG-CHAIN-FATTY-ACID--COA LIGASE; 1.
DR   Pfam; PF00501; AMP-binding; 1.
DR   SUPFAM; SSF56801; Acetyl-CoA synthetase-like; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   1: Evidence at protein level;
KW   Ligase {ECO:0000313|EMBL:AEE34324.1}; Membrane {ECO:0000256|SAM:Phobius};
KW   Proteomics identification {ECO:0007829|PeptideAtlas:F4I9T8,
KW   ECO:0007829|ProteomicsDB:F4I9T8};
KW   Reference proteome {ECO:0000313|Proteomes:UP000006548};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        468..486
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          62..424
FT                   /note="AMP-dependent synthetase/ligase"
FT                   /evidence="ECO:0000259|Pfam:PF00501"
SQ   SEQUENCE   495 AA;  54299 MW;  BEB995F48E001ADF CRC64;
     MITAALHEPQ IHKPTDTSVV SDDVLPHSPP TPRIFRSKLP DIDIPNHLPL HTYCFEKLSS
     VSDKPCLIVG STGKSYTYGE THLICRRVAS GLYKLGIRKG DVIMILLQNS AEFVFSFMGA
     SMIGAVSTTA NPFYTSQELY KQLKSSGAKL IITHSQYVDK LKNLGENLTL ITTDEPTPEN
     CLPFSTLITD DETNPFQETV DIGGDDAAAL PFSSGTTGLP KGVVLTHKSL ITSVAQQVDG
     DNPNLYLKSN DVILCVLPLF HIYSLNSVLL NSLRSGATVL LMHKFEIGAL LDLIQRHRVT
     IAALVPPLVI ALAKNPTVNS YDLSSVRFVL SGAAPLGKEL QDSLRRRLPQ AILGQGYGMT
     EAGPVLSMSL GFAKEPIPTK SGSCGTVVRN AELKVVHLET RLSLGYNQPG EICIRGQQIM
     KEYLNDPEAT SATIDEEGWL HTGDIGYVDE DDEIFIVDRL KEVIKFKGFQ ASPFIFIVLF
     IFNKCLHLKK IYIMI
//