Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

RNA-binding protein Y14

Gene

Y14

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGO-Y14 heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGO-Y14 heterodimer interacts with the EJC key regulator PYM leading to EJC disassembly in the cytoplasm (By similarity). Can increase in vitro the expression from reporter constructs that contain leader introns required for the expression of different genes. In association with MAGO and PYM, participates in intron-mediated enhancement of gene expression (PubMed:21676911). The MAGO-Y14 heterodimer works synergistically with the NMD pathway to regulate male gametophyte development (PubMed:26867216).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionRNA-binding
Biological processmRNA processing, mRNA splicing, mRNA transport, Nonsense-mediated mRNA decay, Translation regulation, Transport

Enzyme and pathway databases

ReactomeiR-ATH-72163. mRNA Splicing - Major Pathway.
R-ATH-72187. mRNA 3'-end processing.
R-ATH-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
RNA-binding protein Y14Curated
Short name:
AtY141 Publication
Alternative name(s):
RNA-binding protein 8ACurated
Gene namesi
Name:Y141 Publication
Synonyms:RBM81 Publication
Ordered Locus Names:At1g51510Imported
ORF Names:F5D21.5Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G51510.
TAIRilocus:2033888. AT1G51510.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: InterPro
  • exon-exon junction complex Source: TAIR
  • nuclear body Source: TAIR
  • nucleolus Source: TAIR
  • nucleoplasm Source: TAIR
  • nucleus Source: InterPro

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004401241 – 202RNA-binding protein Y14Add BLAST202

Expressioni

Tissue specificityi

Expressed in root and shoot meristems, cotyledons, vascular tissues of leaves, receptacle of flowers and siliques, and pollen grains.1 Publication

Interactioni

Subunit structurei

Heterodimer with MAGO (PubMed:24416299, PubMed:19435936, PubMed:26867216). Part of the mRNA splicing-dependent exon junction complex (EJC); the core complex contains MLN51/CASC3, EIF4A3, MAGO and Y14 (Probable). Interacts with PYM (PubMed:16953428, PubMed:21676911). The interaction with PYM is direct and dissociates the EJC from spliced mRNAs (Probable). Weekly interacts with EIF4A3 (PubMed:19435936).2 Publications5 Publications

Protein-protein interaction databases

STRINGi3702.AT1G51510.1.

Structurei

3D structure databases

SMRiF4I9J7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 173RRMPROSITE-ProRule annotationAdd BLAST79

Sequence similaritiesi

Belongs to the RBM8A family.Curated

Phylogenomic databases

eggNOGiKOG0130. Eukaryota.
ENOG4111IHM. LUCA.
KOiK12876.
OMAiISAMNGA.
OrthoDBiEOG09360QWY.

Family and domain databases

CDDicd12324. RRM_RBM8. 1 hit.
InterProiView protein in InterPro
IPR008111. RNA-bd_8.
IPR000504. RRM_dom.
IPR033744. RRM_RBM8.
PfamiView protein in Pfam
PF00076. RRM_1. 1 hit.
PRINTSiPR01738. RNABINDINGM8.
SMARTiView protein in SMART
SM00360. RRM. 1 hit.
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiView protein in PROSITE
PS50102. RRM. 1 hit.

Sequencei

Sequence statusi: Complete.

F4I9J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANIESEAVD FEPEEDDLMD EEGTAIDGAD VSPRAGHPRL KSAIAGANGE
60 70 80 90 100
SAKKTKGRGF REEKDSDRQR RLSSRDFESL GSDGRPGPQR SVEGWIILVS
110 120 130 140 150
GVHEETQEED ITNAFGDFGE IKNLNLNLDR RSGYVKGYAL IEYEKKEEAQ
160 170 180 190 200
SAISAMNGAE LLTQNVSVDW AFSSGPSGGE SYRRKNSRYG RSQRSRSPRR

RY
Length:202
Mass (Da):22,438
Last modified:June 28, 2011 - v1
Checksum:i0DD406A25A360978
GO

Sequence cautioni

The sequence AAG52616 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAK73941 differs from that shown. Reason: Frameshift at position 88.Curated
The sequence AAM16181 differs from that shown. Reason: Frameshift at position 88.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62E → D in AAM61070 (Ref. 5) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
KF051012 mRNA. Translation: AHX83798.1.
AC024261 Genomic DNA. Translation: AAG52616.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE32675.1.
AY045583 mRNA. Translation: AAK73941.1. Frameshift.
AY094025 mRNA. Translation: AAM16181.1. Frameshift.
AY084501 mRNA. Translation: AAM61070.1.
PIRiE96553.
RefSeqiNP_564591.1. NM_104029.4.
UniGeneiAt.18276.

Genome annotation databases

EnsemblPlantsiAT1G51510.1; AT1G51510.1; AT1G51510.
GeneIDi841576.
GrameneiAT1G51510.1; AT1G51510.1; AT1G51510.
KEGGiath:AT1G51510.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiY14_ARATH
AccessioniPrimary (citable) accession number: F4I9J7
Secondary accession number(s): Q8LG23, Q94AZ6, Q9C8K3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2017
Last sequence update: June 28, 2011
Last modified: July 5, 2017
This is version 59 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families