SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

F4I7I0

- ALAT1_ARATH

UniProt

F4I7I0 - ALAT1_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Alanine aminotransferase 1, mitochondrial
Gene
ALAAT1, AOAT4, At1g17290, T13M22.3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Major alanine aminotransferase in roots.1 Publication

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pyridoxal phosphate By similarity.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: TAIR
  2. L-alanine:2-oxoglutarate aminotransferase activity Source: TAIR
  3. pyridoxal phosphate binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. L-alanine catabolic process, by transamination Source: TAIR
  2. biosynthetic process Source: InterPro
  3. response to cadmium ion Source: TAIR
  4. response to hypoxia Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aminotransferase, Transferase

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00322.
UPA00528; UER00586.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine aminotransferase 1, mitochondrial (EC:2.6.1.2)
Short name:
AtAlaAT1
Short name:
AtAlaATc
Alternative name(s):
Alanine-2-oxoglutarate aminotransferase 4 (EC:2.6.1.-)
Gene namesi
Name:ALAAT1
Synonyms:AOAT4
Ordered Locus Names:At1g17290
ORF Names:T13M22.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G17290.

Subcellular locationi

Mitochondrion 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Strong overall decrease of alanine aminotransferase activity, especially in roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4747Mitochondrion Reviewed prediction
Add
BLAST
Chaini48 – 543496Alanine aminotransferase 1, mitochondrial
PRO_0000416042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei360 – 3601N6-(pyridoxal phosphate)lysine By similarity

Post-translational modificationi

The N-terminus is blocked By similarity.

Proteomic databases

PRIDEiF4I7I0.

Expressioni

Tissue specificityi

Mostly expressed in roots and shoots, mostly in vascular tissues, and, to a lower extent, in flowers and leaves.2 Publications

Inductioni

Rapidly induced upon low-oxygen stress in roots and shoots. Induced by cadmium (at protein level).1 Publication

Interactioni

Subunit structurei

Homodimer By similarity.

Protein-protein interaction databases

IntActiF4I7I0. 1 interaction.
STRINGi3702.AT1G17290.1-P.

Structurei

3D structure databases

ProteinModelPortaliF4I7I0.
SMRiF4I7I0. Positions 66-542.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000215020.
KOiK00814.
OMAiHAKFTLE.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F4I7I0-1 [UniParc]FASTAAdd to Basket

« Hide

MRRFVIGQAK NLIDQSRRRQ LHHHKNLSFV SLIPPFSAPS DSSSRHLSSS    50
SSSDMSASDS SSSLPVTLDT INPKVIKCEY AVRGEIVNIA QKLQEDLKTN 100
KDAYPFDEII YCNIGNPQSL GQQPITFFRE VLALCSYTAL LDESATHGLF 150
SSDSIERAWK ILDQIPGRAT GAYSHSQGIK GLRDAIADGI EARDGFPADP 200
NDIFMTDGAS PGVHMMMQLL ITSEKDGILC PIPQYPLYSA SIALHGGTLV 250
PYYLDEASGW GLEISELKKQ LEDARSKGIT VRALAVINPG NPTGQVLSEE 300
NQRDVVKFCK QEGLVLLADE VYQENVYVPD KKFHSFKKVA RSMGYGEKDL 350
ALVSFQSVSK GYYGECGKRG GYMEVTGFTS DVREQIYKMA SVNLCSNISG 400
QILASLIMSP PKPGDDSYES YIAEKDGILS SLARRAKTLE EALNKLEGVT 450
CNRAEGAMYL FPCLHLPQKA IAAAEAEKTA PDNFYCKRLL KATGIVVVPG 500
SGFRQVPGTW HFRCTILPQE DKIPAIVDRL TAFHQSFMDE FRD 543
Length:543
Mass (Da):59,821
Last modified:June 28, 2011 - v1
Checksum:i4ED08F2CA3414E4B
GO

Sequence cautioni

The sequence BAD94892.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti307 – 3071K → N in AAF82782. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF275372 mRNA. Translation: AAF82782.1.
AC026479 Genomic DNA. Translation: AAF79891.1.
CP002684 Genomic DNA. Translation: AEE29570.1.
AY039970 mRNA. Translation: AAK64147.2.
AK221072 mRNA. Translation: BAD94892.1. Different initiation.
PIRiD86309.
RefSeqiNP_173173.3. NM_101591.5.
UniGeneiAt.23768.

Genome annotation databases

EnsemblPlantsiAT1G17290.1; AT1G17290.1; AT1G17290.
GeneIDi838301.
KEGGiath:AT1G17290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF275372 mRNA. Translation: AAF82782.1 .
AC026479 Genomic DNA. Translation: AAF79891.1 .
CP002684 Genomic DNA. Translation: AEE29570.1 .
AY039970 mRNA. Translation: AAK64147.2 .
AK221072 mRNA. Translation: BAD94892.1 . Different initiation.
PIRi D86309.
RefSeqi NP_173173.3. NM_101591.5.
UniGenei At.23768.

3D structure databases

ProteinModelPortali F4I7I0.
SMRi F4I7I0. Positions 66-542.
ModBasei Search...

Protein-protein interaction databases

IntActi F4I7I0. 1 interaction.
STRINGi 3702.AT1G17290.1-P.

Proteomic databases

PRIDEi F4I7I0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G17290.1 ; AT1G17290.1 ; AT1G17290 .
GeneIDi 838301.
KEGGi ath:AT1G17290.

Organism-specific databases

GeneFarmi 4386.
TAIRi AT1G17290.

Phylogenomic databases

HOGENOMi HOG000215020.
KOi K00814.
OMAi HAKFTLE.

Enzyme and pathway databases

UniPathwayi UPA00322 .
UPA00528 ; UER00586 .

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and characterization of Arabidopsis alanine aminotransferase genes."
    Ismond K.P., Dennis E.S., Dolferus R.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. C24.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 75-543.
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-543.
    Strain: cv. Columbia.
  5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 448-543.
    Strain: cv. Columbia.
  6. "Identification of photorespiratory glutamate:glyoxylate aminotransferase (GGAT) gene in Arabidopsis."
    Igarashi D., Miwa T., Seki M., Kobayashi M., Kato T., Tabata S., Shinozaki K., Ohsumi C.
    Plant J. 33:975-987(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
    Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
    Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Landsberg erecta.
  8. "The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
    Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
    Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
    Strain: cv. Columbia.
  9. "Alanine aminotransferase catalyses the breakdown of alanine after hypoxia in Arabidopsis thaliana."
    Miyashita Y., Dolferus R., Ismond K.P., Good A.G.
    Plant J. 49:1108-1121(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION BY HYPOXIC STRESS, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiALAT1_ARATH
AccessioniPrimary (citable) accession number: F4I7I0
Secondary accession number(s): Q56Z96
, Q94BP8, Q9LKJ4, Q9LN15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: June 28, 2011
Last modified: May 14, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi