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Protein

Alanine aminotransferase 1, mitochondrial

Gene

ALAAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Major alanine aminotransferase in roots.1 Publication

Catalytic activityi

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactori

Pathwayi: C4 acid pathway

This protein is involved in the pathway C4 acid pathway, which is part of Photosynthesis.
View all proteins of this organism that are known to be involved in the pathway C4 acid pathway and in Photosynthesis.

Pathwayi: L-alanine degradation via transaminase pathway

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from L-alanine.
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine aminotransferase 1, mitochondrial (ALAAT1), Alanine aminotransferase 2, mitochondrial (ALAAT2), Glutamate--glyoxylate aminotransferase 2 (GGAT2), Glutamate--glyoxylate aminotransferase 1 (GGAT1)
This subpathway is part of the pathway L-alanine degradation via transaminase pathway, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from L-alanine, the pathway L-alanine degradation via transaminase pathway and in Amino-acid degradation.

GO - Molecular functioni

  • ATP binding Source: TAIR
  • L-alanine:2-oxoglutarate aminotransferase activity Source: TAIR
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • biosynthetic process Source: InterPro
  • L-alanine catabolic process, by transamination Source: TAIR
  • response to cadmium ion Source: TAIR
  • response to hypoxia Source: TAIR

Keywordsi

Molecular functionAminotransferase, Transferase
LigandPyridoxal phosphate

Enzyme and pathway databases

UniPathwayiUPA00322
UPA00528; UER00586

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine aminotransferase 1, mitochondrial (EC:2.6.1.2)
Short name:
AtAlaAT1
Short name:
AtAlaATc
Alternative name(s):
Alanine-2-oxoglutarate aminotransferase 4 (EC:2.6.1.-)
Gene namesi
Name:ALAAT1
Synonyms:AOAT4
Ordered Locus Names:At1g17290
ORF Names:T13M22.3
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G17290
TAIRilocus:2195808 AT1G17290

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Disruption phenotypei

Strong overall decrease of alanine aminotransferase activity, especially in roots.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 55MitochondrionCombined sourcesAdd BLAST55
ChainiPRO_000041604256 – 543Alanine aminotransferase 1, mitochondrialAdd BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei56N-acetylserineCombined sources1
Modified residuei360N6-(pyridoxal phosphate)lysineBy similarity1

Post-translational modificationi

The N-terminus is blocked.By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiF4I7I0
PRIDEiF4I7I0
ProMEXiF4I7I0

PTM databases

iPTMnetiF4I7I0
SwissPalmiF4I7I0

Expressioni

Tissue specificityi

Mostly expressed in roots and shoots, mostly in vascular tissues, and, to a lower extent, in flowers and leaves.2 Publications

Inductioni

Rapidly induced upon low-oxygen stress in roots and shoots.1 Publication

Gene expression databases

ExpressionAtlasiF4I7I0 baseline and differential
GenevisibleiF4I7I0 AT

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiF4I7I0, 1 interactor
STRINGi3702.AT1G17290.1

Structurei

3D structure databases

ProteinModelPortaliF4I7I0
SMRiF4I7I0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0258 Eukaryota
COG0436 LUCA
HOGENOMiHOG000215020
InParanoidiF4I7I0
KOiK00814
OMAiAPGTEWV
OrthoDBiEOG093607PE

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F4I7I0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRFVIGQAK NLIDQSRRRQ LHHHKNLSFV SLIPPFSAPS DSSSRHLSSS
60 70 80 90 100
SSSDMSASDS SSSLPVTLDT INPKVIKCEY AVRGEIVNIA QKLQEDLKTN
110 120 130 140 150
KDAYPFDEII YCNIGNPQSL GQQPITFFRE VLALCSYTAL LDESATHGLF
160 170 180 190 200
SSDSIERAWK ILDQIPGRAT GAYSHSQGIK GLRDAIADGI EARDGFPADP
210 220 230 240 250
NDIFMTDGAS PGVHMMMQLL ITSEKDGILC PIPQYPLYSA SIALHGGTLV
260 270 280 290 300
PYYLDEASGW GLEISELKKQ LEDARSKGIT VRALAVINPG NPTGQVLSEE
310 320 330 340 350
NQRDVVKFCK QEGLVLLADE VYQENVYVPD KKFHSFKKVA RSMGYGEKDL
360 370 380 390 400
ALVSFQSVSK GYYGECGKRG GYMEVTGFTS DVREQIYKMA SVNLCSNISG
410 420 430 440 450
QILASLIMSP PKPGDDSYES YIAEKDGILS SLARRAKTLE EALNKLEGVT
460 470 480 490 500
CNRAEGAMYL FPCLHLPQKA IAAAEAEKTA PDNFYCKRLL KATGIVVVPG
510 520 530 540
SGFRQVPGTW HFRCTILPQE DKIPAIVDRL TAFHQSFMDE FRD
Length:543
Mass (Da):59,821
Last modified:June 28, 2011 - v1
Checksum:i4ED08F2CA3414E4B
GO

Sequence cautioni

The sequence BAD94892 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti307K → N in AAF82782 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF275372 mRNA Translation: AAF82782.1
AC026479 Genomic DNA Translation: AAF79891.1
CP002684 Genomic DNA Translation: AEE29570.1
AY039970 mRNA Translation: AAK64147.2
AK221072 mRNA Translation: BAD94892.1 Different initiation.
PIRiD86309
RefSeqiNP_173173.3, NM_101591.6
UniGeneiAt.23768

Genome annotation databases

EnsemblPlantsiAT1G17290.1; AT1G17290.1; AT1G17290
GeneIDi838301
GrameneiAT1G17290.1; AT1G17290.1; AT1G17290
KEGGiath:AT1G17290

Similar proteinsi

Entry informationi

Entry nameiALAT1_ARATH
AccessioniPrimary (citable) accession number: F4I7I0
Secondary accession number(s): Q56Z96
, Q94BP8, Q9LKJ4, Q9LN15
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: June 28, 2011
Last modified: May 23, 2018
This is version 56 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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