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F4I7I0

- ALAT1_ARATH

UniProt

F4I7I0 - ALAT1_ARATH

Protein

Alanine aminotransferase 1, mitochondrial

Gene

ALAAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Major alanine aminotransferase in roots.1 Publication

    Catalytic activityi

    L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: TAIR
    2. L-alanine:2-oxoglutarate aminotransferase activity Source: TAIR
    3. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. biosynthetic process Source: InterPro
    2. L-alanine catabolic process, by transamination Source: TAIR
    3. response to cadmium ion Source: TAIR
    4. response to hypoxia Source: TAIR

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    UniPathwayiUPA00322.
    UPA00528; UER00586.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine aminotransferase 1, mitochondrial (EC:2.6.1.2)
    Short name:
    AtAlaAT1
    Short name:
    AtAlaATc
    Alternative name(s):
    Alanine-2-oxoglutarate aminotransferase 4 (EC:2.6.1.-)
    Gene namesi
    Name:ALAAT1
    Synonyms:AOAT4
    Ordered Locus Names:At1g17290
    ORF Names:T13M22.3
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G17290.

    Subcellular locationi

    Mitochondrion 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. mitochondrion Source: TAIR

    Keywords - Cellular componenti

    Mitochondrion

    Pathology & Biotechi

    Disruption phenotypei

    Strong overall decrease of alanine aminotransferase activity, especially in roots.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4747MitochondrionSequence AnalysisAdd
    BLAST
    Chaini48 – 543496Alanine aminotransferase 1, mitochondrialPRO_0000416042Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei360 – 3601N6-(pyridoxal phosphate)lysineBy similarity

    Post-translational modificationi

    The N-terminus is blocked.By similarity

    Proteomic databases

    PRIDEiF4I7I0.

    Expressioni

    Tissue specificityi

    Mostly expressed in roots and shoots, mostly in vascular tissues, and, to a lower extent, in flowers and leaves.2 Publications

    Inductioni

    Rapidly induced upon low-oxygen stress in roots and shoots. Induced by cadmium (at protein level).2 Publications

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    IntActiF4I7I0. 1 interaction.
    STRINGi3702.AT1G17290.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliF4I7I0.
    SMRiF4I7I0. Positions 66-542.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000215020.
    KOiK00814.
    OMAiHAKFTLE.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    F4I7I0-1 [UniParc]FASTAAdd to Basket

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    MRRFVIGQAK NLIDQSRRRQ LHHHKNLSFV SLIPPFSAPS DSSSRHLSSS    50
    SSSDMSASDS SSSLPVTLDT INPKVIKCEY AVRGEIVNIA QKLQEDLKTN 100
    KDAYPFDEII YCNIGNPQSL GQQPITFFRE VLALCSYTAL LDESATHGLF 150
    SSDSIERAWK ILDQIPGRAT GAYSHSQGIK GLRDAIADGI EARDGFPADP 200
    NDIFMTDGAS PGVHMMMQLL ITSEKDGILC PIPQYPLYSA SIALHGGTLV 250
    PYYLDEASGW GLEISELKKQ LEDARSKGIT VRALAVINPG NPTGQVLSEE 300
    NQRDVVKFCK QEGLVLLADE VYQENVYVPD KKFHSFKKVA RSMGYGEKDL 350
    ALVSFQSVSK GYYGECGKRG GYMEVTGFTS DVREQIYKMA SVNLCSNISG 400
    QILASLIMSP PKPGDDSYES YIAEKDGILS SLARRAKTLE EALNKLEGVT 450
    CNRAEGAMYL FPCLHLPQKA IAAAEAEKTA PDNFYCKRLL KATGIVVVPG 500
    SGFRQVPGTW HFRCTILPQE DKIPAIVDRL TAFHQSFMDE FRD 543
    Length:543
    Mass (Da):59,821
    Last modified:June 28, 2011 - v1
    Checksum:i4ED08F2CA3414E4B
    GO

    Sequence cautioni

    The sequence BAD94892.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti307 – 3071K → N in AAF82782. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF275372 mRNA. Translation: AAF82782.1.
    AC026479 Genomic DNA. Translation: AAF79891.1.
    CP002684 Genomic DNA. Translation: AEE29570.1.
    AY039970 mRNA. Translation: AAK64147.2.
    AK221072 mRNA. Translation: BAD94892.1. Different initiation.
    PIRiD86309.
    RefSeqiNP_173173.3. NM_101591.5.
    UniGeneiAt.23768.

    Genome annotation databases

    EnsemblPlantsiAT1G17290.1; AT1G17290.1; AT1G17290.
    GeneIDi838301.
    KEGGiath:AT1G17290.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF275372 mRNA. Translation: AAF82782.1 .
    AC026479 Genomic DNA. Translation: AAF79891.1 .
    CP002684 Genomic DNA. Translation: AEE29570.1 .
    AY039970 mRNA. Translation: AAK64147.2 .
    AK221072 mRNA. Translation: BAD94892.1 . Different initiation.
    PIRi D86309.
    RefSeqi NP_173173.3. NM_101591.5.
    UniGenei At.23768.

    3D structure databases

    ProteinModelPortali F4I7I0.
    SMRi F4I7I0. Positions 66-542.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi F4I7I0. 1 interaction.
    STRINGi 3702.AT1G17290.1-P.

    Proteomic databases

    PRIDEi F4I7I0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G17290.1 ; AT1G17290.1 ; AT1G17290 .
    GeneIDi 838301.
    KEGGi ath:AT1G17290.

    Organism-specific databases

    GeneFarmi 4386.
    TAIRi AT1G17290.

    Phylogenomic databases

    HOGENOMi HOG000215020.
    KOi K00814.
    OMAi HAKFTLE.

    Enzyme and pathway databases

    UniPathwayi UPA00322 .
    UPA00528 ; UER00586 .

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of Arabidopsis alanine aminotransferase genes."
      Ismond K.P., Dennis E.S., Dolferus R.
      Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. C24.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 75-543.
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-543.
      Strain: cv. Columbia.
    5. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 448-543.
      Strain: cv. Columbia.
    6. "Identification of photorespiratory glutamate:glyoxylate aminotransferase (GGAT) gene in Arabidopsis."
      Igarashi D., Miwa T., Seki M., Kobayashi M., Kato T., Tabata S., Shinozaki K., Ohsumi C.
      Plant J. 33:975-987(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
    7. "Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
      Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
      Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
      Strain: cv. Landsberg erecta.
    8. "The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
      Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
      Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
      Strain: cv. Columbia.
    9. "Alanine aminotransferase catalyses the breakdown of alanine after hypoxia in Arabidopsis thaliana."
      Miyashita Y., Dolferus R., Ismond K.P., Good A.G.
      Plant J. 49:1108-1121(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION BY HYPOXIC STRESS, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiALAT1_ARATH
    AccessioniPrimary (citable) accession number: F4I7I0
    Secondary accession number(s): Q56Z96
    , Q94BP8, Q9LKJ4, Q9LN15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2012
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3