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F4I7I0 (ALAT1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 26. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alanine aminotransferase 1, mitochondrial

Short name=AtAlaAT1
Short name=AtAlaATc
EC=2.6.1.2
Alternative name(s):
Alanine-2-oxoglutarate aminotransferase 4
EC=2.6.1.-
Gene names
Name:ALAAT1
Synonyms:AOAT4
Ordered Locus Names:At1g17290
ORF Names:T13M22.3
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length543 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major alanine aminotransferase in roots. Ref.9

Catalytic activity

L-alanine + 2-oxoglutarate = pyruvate + L-glutamate.

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Photosynthesis; C4 acid pathway.

Amino-acid degradation; L-alanine degradation via transaminase pathway; pyruvate from L-alanine: step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion Ref.7.

Tissue specificity

Mostly expressed in roots and shoots, mostly in vascular tissues, and, to a lower extent, in flowers and leaves. Ref.6 Ref.9

Induction

Rapidly induced upon low-oxygen stress in roots and shoots. Induced by cadmium (at protein level). Ref.9

Post-translational modification

The N-terminus is blocked By similarity.

Disruption phenotype

Strong overall decrease of alanine aminotransferase activity, especially in roots. Ref.9

Sequence similarities

Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.

Sequence caution

The sequence BAD94892.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4747Mitochondrion Potential
Chain48 – 543496Alanine aminotransferase 1, mitochondrial
PRO_0000416042

Amino acid modifications

Modified residue3601N6-(pyridoxal phosphate)lysine By similarity

Experimental info

Sequence conflict3071K → N in AAF82782. Ref.1

Sequences

Sequence LengthMass (Da)Tools
F4I7I0 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 4ED08F2CA3414E4B

FASTA54359,821
        10         20         30         40         50         60 
MRRFVIGQAK NLIDQSRRRQ LHHHKNLSFV SLIPPFSAPS DSSSRHLSSS SSSDMSASDS 

        70         80         90        100        110        120 
SSSLPVTLDT INPKVIKCEY AVRGEIVNIA QKLQEDLKTN KDAYPFDEII YCNIGNPQSL 

       130        140        150        160        170        180 
GQQPITFFRE VLALCSYTAL LDESATHGLF SSDSIERAWK ILDQIPGRAT GAYSHSQGIK 

       190        200        210        220        230        240 
GLRDAIADGI EARDGFPADP NDIFMTDGAS PGVHMMMQLL ITSEKDGILC PIPQYPLYSA 

       250        260        270        280        290        300 
SIALHGGTLV PYYLDEASGW GLEISELKKQ LEDARSKGIT VRALAVINPG NPTGQVLSEE 

       310        320        330        340        350        360 
NQRDVVKFCK QEGLVLLADE VYQENVYVPD KKFHSFKKVA RSMGYGEKDL ALVSFQSVSK 

       370        380        390        400        410        420 
GYYGECGKRG GYMEVTGFTS DVREQIYKMA SVNLCSNISG QILASLIMSP PKPGDDSYES 

       430        440        450        460        470        480 
YIAEKDGILS SLARRAKTLE EALNKLEGVT CNRAEGAMYL FPCLHLPQKA IAAAEAEKTA 

       490        500        510        520        530        540 
PDNFYCKRLL KATGIVVVPG SGFRQVPGTW HFRCTILPQE DKIPAIVDRL TAFHQSFMDE 


FRD 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of Arabidopsis alanine aminotransferase genes."
Ismond K.P., Dennis E.S., Dolferus R.
Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. C24.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 75-543.
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-543.
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 448-543.
Strain: cv. Columbia.
[6]"Identification of photorespiratory glutamate:glyoxylate aminotransferase (GGAT) gene in Arabidopsis."
Igarashi D., Miwa T., Seki M., Kobayashi M., Kato T., Tabata S., Shinozaki K., Ohsumi C.
Plant J. 33:975-987(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
[7]"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins."
Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H.
Plant Cell 16:241-256(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
Strain: cv. Landsberg erecta.
[8]"The early responses of Arabidopsis thaliana cells to cadmium exposure explored by protein and metabolite profiling analyses."
Sarry J.-E., Kuhn L., Ducruix C., Lafaye A., Junot C., Hugouvieux V., Jourdain A., Bastien O., Fievet J.B., Vailhen D., Amekraz B., Moulin C., Ezan E., Garin J., Bourguignon J.
Proteomics 6:2180-2198(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION BY CADMIUM [LARGE SCALE ANALYSIS].
Strain: cv. Columbia.
[9]"Alanine aminotransferase catalyses the breakdown of alanine after hypoxia in Arabidopsis thaliana."
Miyashita Y., Dolferus R., Ismond K.P., Good A.G.
Plant J. 49:1108-1121(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, INDUCTION BY HYPOXIC STRESS, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF275372 mRNA. Translation: AAF82782.1.
AC026479 Genomic DNA. Translation: AAF79891.1.
CP002684 Genomic DNA. Translation: AEE29570.1.
AY039970 mRNA. Translation: AAK64147.2.
AK221072 mRNA. Translation: BAD94892.1. Different initiation.
PIRD86309.
RefSeqNP_173173.3. NM_101591.5.
UniGeneAt.23768.

3D structure databases

ProteinModelPortalF4I7I0.
SMRF4I7I0. Positions 66-542.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActF4I7I0. 1 interaction.
STRING3702.AT1G17290.1-P.

Proteomic databases

PRIDEF4I7I0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G17290.1; AT1G17290.1; AT1G17290.
GeneID838301.
KEGGath:AT1G17290.

Organism-specific databases

GeneFarm4386.
TAIRAT1G17290.

Phylogenomic databases

HOGENOMHOG000215020.
KOK00814.
OMAHAKFTLE.

Enzyme and pathway databases

UniPathwayUPA00322.
UPA00528; UER00586.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMSSF53383. SSF53383. 1 hit.
ProtoNetSearch...

Entry information

Entry nameALAT1_ARATH
AccessionPrimary (citable) accession number: F4I7I0
Secondary accession number(s): Q56Z96 expand/collapse secondary AC list , Q94BP8, Q9LKJ4, Q9LN15
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2012
Last sequence update: June 28, 2011
Last modified: May 14, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names