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F4I1L3

- ACC2_ARATH

UniProt

F4I1L3 - ACC2_ARATH

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Protein

Acetyl-CoA carboxylase 2

Gene

ACC2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Multifunctional enzyme that catalyzes the carboxylation of acetyl-CoA, forming malonyl-CoA, which is used in the plastid for fatty acid synthesis and in the cytosol in various biosynthetic pathways including fatty acid elongation.By similarity

Catalytic activityi

ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA.
ATP + biotin-[carboxyl-carrier-protein] + CO2 = ADP + phosphate + carboxy-biotin-[carboxyl-carrier-protein].

Cofactori

Biotin.By similarity
Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi440 – 4401Magnesium or manganese 1PROSITE-ProRule annotation
Metal bindingi454 – 4541Magnesium or manganese 1PROSITE-ProRule annotation
Metal bindingi454 – 4541Magnesium or manganese 2PROSITE-ProRule annotation
Metal bindingi456 – 4561Magnesium or manganese 2PROSITE-ProRule annotation
Active sitei458 – 4581By similarity
Binding sitei1841 – 18411Coenzyme ABy similarity
Binding sitei2142 – 21421Coenzyme ABy similarity
Binding sitei2144 – 21441Coenzyme ABy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi317 – 37458ATPPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. acetyl-CoA carboxylase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW
  3. biotin carboxylase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
  2. malonyl-CoA biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

ATP-binding, Biotin, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_185257. Defective HLCS causes multiple carboxylase deficiency.
REACT_187915. Biotin transport and metabolism.
UniPathwayiUPA00655; UER00711.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-CoA carboxylase 2 (EC:6.4.1.2)
Including the following 1 domains:
Biotin carboxylase (EC:6.3.4.14)
Gene namesi
Name:ACC2
Ordered Locus Names:At1g36180
ORF Names:F15C21.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G36180.

Subcellular locationi

Cytoplasmcytosol Curated

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 23552355Acetyl-CoA carboxylase 2PRO_0000412212Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1133 – 11331PhosphothreonineBy similarity
Modified residuei1293 – 12931PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiF4I1L3.

Expressioni

Tissue specificityi

Widely expressed at low levels.2 Publications

Gene expression databases

ExpressionAtlasiF4I1L3. baseline and differential.

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

BioGridi25754. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliF4I1L3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 645508Biotin carboxylationAdd
BLAST
Domaini291 – 485195ATP-graspPROSITE-ProRule annotationAdd
BLAST
Domaini779 – 84567Biotinyl-bindingAdd
BLAST
Domaini1619 – 2211593CarboxyltransferaseAdd
BLAST

Sequence similaritiesi

Contains 1 ATP-grasp domain.PROSITE-ProRule annotation
Contains 1 biotin carboxylation domain.Curated
Contains 1 biotinyl-binding domain.Curated
Contains 1 carboxyltransferase domain.Curated

Phylogenomic databases

HOGENOMiHOG000214115.
InParanoidiF4I1L3.
OMAiHICAGET.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.226.10. 3 hits.
InterProiIPR013537. AcCoA_COase_cen.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR000022. Carboxyl_trans.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR029045. ClpP/crotonase-like_dom.
IPR011763. COA_CT_C.
IPR011762. COA_CT_N.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view]
PfamiPF08326. ACC_central. 1 hit.
PF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF01039. Carboxyl_trans. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view]
SMARTiSM00878. Biotin_carb_C. 1 hit.
[Graphical view]
SUPFAMiSSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52096. SSF52096. 2 hits.
SSF52440. SSF52440. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS50989. COA_CT_CTER. 1 hit.
PS50980. COA_CT_NTER. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: F4I1L3-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEMRALGSSC STGNGGSAPI TLTNISPWIT TVFPSTVKLR SSLRTFKGVS
60 70 80 90 100
SRVRTFKGVS STRVLSRTKQ QFPLFCFLNP DPISFLENDV SEAERTVVLP
110 120 130 140 150
DGSVNGAGSV NGYHSDVVPG RNVAEVNEFC KALGGKRPIH SILVATNGMA
160 170 180 190 200
AVKFIRSVRT WAYETFGSEK AVKLVAMATP EDMRINAEHI RIADQFVEVP
210 220 230 240 250
GGTNNNNYAN VQLIVEMAEV TRVDAVWPGW GHASENPELP DALKEKGIIF
260 270 280 290 300
LGPPADSMIA LGDKIGSSLI AQAADVPTLP WSGSHVKIPP GRSLVTVPEE
310 320 330 340 350
IYKKACVYTT EEAIASCQVV GYPAMIKASW GGGGKGIRKV HNDDEVRALF
360 370 380 390 400
KQVQGEVPGS PIFIMKVASQ SRHLEAQLLC DQYGNVAALH SRDCSVQRRH
410 420 430 440 450
QKIIEEGPIT VAPQETIKKL EQAARRLAKS VNYVGAATVE YLYSMDTGEY
460 470 480 490 500
YFLELNPRLQ VEHPVTEWIA EVNLPAAQVA VGMGIPLWQI PEIRRFYGME
510 520 530 540 550
HGGGYDSWRK TSVVASPFDF DEAESLRPKG HCVAVRVTSE DPDDGFKPTS
560 570 580 590 600
GEIQELSFKS KPNMWSYFSV KSGGGIHEFS DSQFGHVFAF GESRSVAIAN
610 620 630 640 650
MVLALKEIQI RGDIRTNVDY TIDLLHASDY RENKIHTGWL DSRIAMRVRA
660 670 680 690 700
ERPPWYLSVV GGALYKASTT SSAVVSDYVG YLEKGQIPPK HISLVHSQVS
710 720 730 740 750
LNIEGSKYTI DVVRGGSGTY RLRMSNSEVV AEIHTLRDGG LLMQLDGKSH
760 770 780 790 800
VIYAKEEATG TRLLIDGRTC LLQNDHDPSK LMAETPCKLL RYLVSDNSSI
810 820 830 840 850
DTDTPYAEVE VMKMCMPLIS PASGVIHFKL SEGQAMQAGE LIAKLDLDDP
860 870 880 890 900
SAVRKAKPFR GSFPRLGLPT AISGKVHQRC AATLNAARMI LAGYDHKVDE
910 920 930 940 950
VLQDLLNCLD SPELPFLQWQ ECFAVLATRL PKDLRNMLEL KYKEFEIISK
960 970 980 990 1000
TSLTPDFPAK LLKGILEAHL SSCDEKERGS LERLIEPLMS LVKSYEGGRE
1010 1020 1030 1040 1050
SHARLIVHSL FEEYLSVEEL FNDNMLADVI ERMRQQYKKD RLKIVDIVLS
1060 1070 1080 1090 1100
HQGIIHKNKL VLRLMEQLVY PNPAAYREKL IRFSALNHTN YSQLALKASQ
1110 1120 1130 1140 1150
LLEQTKRSEL RSNIARSLSE LEMFTEAGEN MDTPKRKSAI SETMENLVSS
1160 1170 1180 1190 1200
SLAVEDALVG LFDHSDHTLQ RRVVETYIHR LYQPYVVKES VRMQWHQSGV
1210 1220 1230 1240 1250
IASWEFLEHF ERKNTGPDDH EISEKGIVAK SSKRKRGTMV IIKSLQFLPS
1260 1270 1280 1290 1300
IINASLRETN HSHCEYARAP LSGNMMHIAV VGINNQMSLL QDSGDEDQTQ
1310 1320 1330 1340 1350
ERVNKLAKIL KEEEVSLTLC SAGVGVISCI IQRDEGRTPM RHSFHWLMEK
1360 1370 1380 1390 1400
QYYVEEPLLR HVEPPLSVYL ELDKLKGYSN IQYSPSRDRQ WHMYSVTDRP
1410 1420 1430 1440 1450
VPIKRMFLRS LVRQTTMNDG FLLQQGQDYQ LSQTVLSMAF TSKCILRSLM
1460 1470 1480 1490 1500
NAMEELELNA HNAAMKPDHA HMFLCILREQ QIDDLVPYPR RFEVNAEDEE
1510 1520 1530 1540 1550
TTVETILEEA TQEIHRSVGV RMHALGVCEW EVRLWLVSSG LANGAWRVVV
1560 1570 1580 1590 1600
ANVTGRTCTV HIYREVEATG RNSLIYHSIT KKGPLHGTLI NGQYKPLNNL
1610 1620 1630 1640 1650
DRKRLAARRS NTTYCYDFPL AFETALELNW ASQHSGVRKP CKNRLINVKE
1660 1670 1680 1690 1700
LVFSNTEGSL GTSLIPVERP AGLNDIGMVA WILEMSTPEF PMGRKLLIVA
1710 1720 1730 1740 1750
NDVTFKAGSF GPREDAFFLA VTELACTKKL PLIYLAANSG ARLGVAEEVK
1760 1770 1780 1790 1800
ACFKVGWSDE VSPGNDFQYI YLSSEDYARI GSSVIAHEVK LPSGETRWVI
1810 1820 1830 1840 1850
DTIVGKEDGL GVENLTGSGA IAGAYSRAYN ETFTLTFVSG RSVGIGAYLA
1860 1870 1880 1890 1900
RLGMRCIQRL DQPIILTGFS TLNKLLGREV YSSHMQLGGP KIMGTNGVVH
1910 1920 1930 1940 1950
LTVSDDLEGV SAILNWLSYI PAYVGGPLPV LAPLDPPERT VEYIPENSCD
1960 1970 1980 1990 2000
PRAAIAGIND NTGKWLGGIF DKNSFVETLE GWARTVVTGR AKLGGIPIGV
2010 2020 2030 2040 2050
VAVETQTVMH VIPADPGQLD SHERVVPQAG QVWFPDSAAK TAQALMDFNR
2060 2070 2080 2090 2100
EQLPLFIIAN WRGFSGGQRD LFEGILQAGS AIVENLRTYR QPVFVYIPMM
2110 2120 2130 2140 2150
GELRGGAWVV VDSQINSDYI EMYADETARG NVLEPEGMIE IKFRRKELLE
2160 2170 2180 2190 2200
CMGRLDQTLI NLKANIQDAK RNKAYANIEL LQKQIKTREK QLLPVYTQIA
2210 2220 2230 2240 2250
TKFAELHDTS MRMAAKGVIK SVVEWSGSRS FFYKKLYRRI AESSLVRNIR
2260 2270 2280 2290 2300
KASGDILSYK SAMGLIQDWF RKSEIAKGKE EAWTDDQLFF TWKDNVSNYE
2310 2320 2330 2340 2350
QKLSELRTQK LLNQLAEIGN SSDLQALPQG LANLLNKVDL SRREELVDAI

RKVLG
Length:2,355
Mass (Da):262,729
Last modified:June 28, 2011 - v1
Checksum:iD3E322E33E847E0A
GO

Sequence cautioni

The sequence AAG40564.1 differs from that shown. Reason: Erroneous gene model prediction.
The sequence AAG51252.1 differs from that shown. Reason: Erroneous gene model prediction.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1855 – 18551R → K in AAG40564. (PubMed:7551584)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF062308 Genomic DNA. Translation: AAG40564.1. Sequence problems.
AC025781 Genomic DNA. Translation: AAG51252.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE31851.1.
PIRiE86483.
RefSeqiNP_174850.4. NM_103314.4. [F4I1L3-1]
UniGeneiAt.51973.

Genome annotation databases

EnsemblPlantsiAT1G36180.1; AT1G36180.1; AT1G36180. [F4I1L3-1]
GeneIDi840522.
KEGGiath:AT1G36180.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF062308 Genomic DNA. Translation: AAG40564.1 . Sequence problems.
AC025781 Genomic DNA. Translation: AAG51252.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE31851.1 .
PIRi E86483.
RefSeqi NP_174850.4. NM_103314.4. [F4I1L3-1 ]
UniGenei At.51973.

3D structure databases

ProteinModelPortali F4I1L3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 25754. 1 interaction.

Proteomic databases

PRIDEi F4I1L3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G36180.1 ; AT1G36180.1 ; AT1G36180 . [F4I1L3-1 ]
GeneIDi 840522.
KEGGi ath:AT1G36180.

Organism-specific databases

TAIRi AT1G36180.

Phylogenomic databases

HOGENOMi HOG000214115.
InParanoidi F4I1L3.
OMAi HICAGET.

Enzyme and pathway databases

UniPathwayi UPA00655 ; UER00711 .
Reactomei REACT_185257. Defective HLCS causes multiple carboxylase deficiency.
REACT_187915. Biotin transport and metabolism.

Gene expression databases

ExpressionAtlasi F4I1L3. baseline and differential.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.226.10. 3 hits.
InterProi IPR013537. AcCoA_COase_cen.
IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR001882. Biotin_BS.
IPR011764. Biotin_carboxylation_dom.
IPR005482. Biotin_COase_C.
IPR000089. Biotin_lipoyl.
IPR005481. CarbamoylP_synth_lsu_N.
IPR000022. Carboxyl_trans.
IPR005479. CbamoylP_synth_lsu-like_ATP-bd.
IPR029045. ClpP/crotonase-like_dom.
IPR011763. COA_CT_C.
IPR011762. COA_CT_N.
IPR016185. PreATP-grasp_dom.
IPR011054. Rudment_hybrid_motif.
IPR011053. Single_hybrid_motif.
[Graphical view ]
Pfami PF08326. ACC_central. 1 hit.
PF02785. Biotin_carb_C. 1 hit.
PF00364. Biotin_lipoyl. 1 hit.
PF01039. Carboxyl_trans. 1 hit.
PF00289. CPSase_L_chain. 1 hit.
PF02786. CPSase_L_D2. 1 hit.
[Graphical view ]
SMARTi SM00878. Biotin_carb_C. 1 hit.
[Graphical view ]
SUPFAMi SSF51230. SSF51230. 1 hit.
SSF51246. SSF51246. 1 hit.
SSF52096. SSF52096. 2 hits.
SSF52440. SSF52440. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS50979. BC. 1 hit.
PS00188. BIOTIN. 1 hit.
PS50968. BIOTINYL_LIPOYL. 1 hit.
PS50989. COA_CT_CTER. 1 hit.
PS50980. COA_CT_NTER. 1 hit.
PS00867. CPSASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genomic organization of 251 kDa acetyl-CoA carboxylase genes in Arabidopsis: tandem gene duplication has made two differentially expressed isozymes."
    Yanai Y., Kawasaki T., Shimada H., Wurtele E.S., Nikolau B.J., Ichikawa N.
    Plant Cell Physiol. 36:779-787(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Multifunctional acetyl-CoA carboxylase 1 is essential for very long chain fatty acid elongation and embryo development in Arabidopsis."
    Baud S., Guyon V., Kronenberger J., Wuilleme S., Miquel M., Caboche M., Lepiniec L., Rochat C.
    Plant J. 33:75-86(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
    Strain: cv. Wassilewskija.

Entry informationi

Entry nameiACC2_ARATH
AccessioniPrimary (citable) accession number: F4I1L3
Secondary accession number(s): Q9C8G0, Q9FR96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: June 28, 2011
Last modified: October 29, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3