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F4HW02 (GAT1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GABA transporter 1

Short name=AtGAT1
Alternative name(s):
Bidirectional amino acid transporter 1
Gene names
Name:GAT1
Synonyms:BAT1
Ordered Locus Names:At1g08230
ORF Names:T23G18.9
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length451 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

High affinity gamma-aminobutyric acid (GABA) transporter probably involved in GABA uptake into cells. When expressed in a heterologous system (Xenopus oocytes), imports GABA, butylamine, beta- and L-Alanine, 5-aminovaleric acid, 6-aminocaproic acid and 8-aminocaprylic acid, but does not mediate the transport of proline or glycine betaine. Ref.4 Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein Probable. Note: Plasma membrane. Ref.4

Tissue specificity

Highly expressed in flowers and at lower levels in roots, leaves and stems. Ref.4 Ref.5

Induction

By wounding and senescence. Ref.4

Sequence similarities

Belongs to the amino acid/polyamine transporter 2 family. Amino acid/auxin permease (AAAP) (TC 2.A.18) subfamily. [View classification]

Biophysicochemical properties

Kinetic parameters:

KM=10 µM for GABA Ref.4

pH dependence:

Optimum pH is 4.5-5.5.

Sequence caution

The sequence AAF18251.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence EF183286 differs from that shown. Reason: Frameshift at position 286.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 451451GABA transporter 1
PRO_0000418998

Regions

Transmembrane35 – 5521Helical; Potential
Transmembrane57 – 7721Helical; Potential
Transmembrane115 – 13521Helical; Potential
Transmembrane153 – 17321Helical; Potential
Transmembrane182 – 20221Helical; Potential
Transmembrane222 – 24221Helical; Potential
Transmembrane262 – 28221Helical; Potential
Transmembrane308 – 32821Helical; Potential
Transmembrane356 – 37621Helical; Potential
Transmembrane382 – 40221Helical; Potential
Transmembrane411 – 43121Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
F4HW02 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 1DBC49E86E3088C5

FASTA45149,695
        10         20         30         40         50         60 
MGGEERSGDG EKRGEEVVDA GSLFVLKSKG TWWHCGFHLT TSIVAPALLS LPYAFKFLGW 

        70         80         90        100        110        120 
AAGISCLVGG AAVTFYSYTL LSLTLEHHAS LGNRYLRFRD MAHHILSPKW GRYYVGPIQM 

       130        140        150        160        170        180 
AVCYGVVIAN ALLGGQCLKA MYLVVQPNGE MKLFEFVIIF GCLLLVLAQF PSFHSLRYIN 

       190        200        210        220        230        240 
SLSLLLCLLY SASAAAASIY IGKEPNAPEK DYTIVGDPET RVFGIFNAMA IIATTYGNGI 

       250        260        270        280        290        300 
IPEIQATISA PVKGKMMKGL CMCYLVVIMT FFTVAITGYW AFGKKANGLI FTNFLNAETN 

       310        320        330        340        350        360 
HYFVPTWFIF LVNLFTVLQL SAVAVVYLQP INDILESVIS DPTKKEFSIR NVIPRLVVRS 

       370        380        390        400        410        420 
LFVVMATIVA AMLPFFGDVN SLLGAFGFIP LDFVLPVVFF NFTFKPSKKS FIFWINTVIA 

       430        440        450 
VVFSCLGVIA MVAAVRQIII DANTYKLFAD V 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Experimental validation of novel genes predicted in the un-annotated regions of the Arabidopsis genome."
Moskal W.A. Jr., Wu H.C., Underwood B.A., Wang W., Town C.D., Xiao Y.-L.
BMC Genomics 8:18-18(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"AtGAT1, a high affinity transporter for gamma-aminobutyric acid in Arabidopsis thaliana."
Meyer A., Eskandari S., Grallath S., Rentsch D.
J. Biol. Chem. 281:7197-7204(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
[5]"BAT1, a bidirectional amino acid transporter in Arabidopsis."
Duendar E., Bush D.R.
Planta 229:1047-1056(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC011438 Genomic DNA. Translation: AAF18251.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28264.1.
EF183286 mRNA. No translation available.
RefSeqNP_001077487.1. NM_001084018.2.
UniGeneAt.42270.
At.73273.
At.75057.

3D structure databases

ProteinModelPortalF4HW02.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEF4HW02.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G08230.2; AT1G08230.2; AT1G08230.
GeneID837344.
KEGGath:AT1G08230.

Organism-specific databases

TAIRAT1G08230.

Phylogenomic databases

OMAAQLPSFH.

Gene expression databases

ArrayExpressF4HW02.

Family and domain databases

InterProIPR013057. AA_transpt_TM.
[Graphical view]
PfamPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGAT1_ARATH
AccessionPrimary (citable) accession number: F4HW02
Secondary accession number(s): Q9SGD7
Entry history
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: June 28, 2011
Last modified: February 19, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names