Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GABA transporter 1

Gene

GAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

High affinity gamma-aminobutyric acid (GABA) transporter probably involved in GABA uptake into cells. When expressed in a heterologous system (Xenopus oocytes), imports GABA, butylamine, beta- and L-Alanine, 5-aminovaleric acid, 6-aminocaproic acid and 8-aminocaprylic acid, but does not mediate the transport of proline or glycine betaine.2 Publications

Kineticsi

  1. KM=10 µM for GABA1 Publication

    pH dependencei

    Optimum pH is 4.5-5.5.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    • amino acid transmembrane transport Source: GO_Central
    • gamma-aminobutyric acid transport Source: TAIR

    Keywordsi

    Biological processAmino-acid transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GABA transporter 1
    Short name:
    AtGAT1
    Alternative name(s):
    Bidirectional amino acid transporter 1
    Gene namesi
    Name:GAT1
    Synonyms:BAT1
    Ordered Locus Names:At1g08230
    ORF Names:T23G18.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 1

    Organism-specific databases

    AraportiAT1G08230
    TAIRilocus:2199978 AT1G08230

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei35 – 55HelicalSequence analysisAdd BLAST21
    Transmembranei57 – 77HelicalSequence analysisAdd BLAST21
    Transmembranei115 – 135HelicalSequence analysisAdd BLAST21
    Transmembranei153 – 173HelicalSequence analysisAdd BLAST21
    Transmembranei182 – 202HelicalSequence analysisAdd BLAST21
    Transmembranei222 – 242HelicalSequence analysisAdd BLAST21
    Transmembranei262 – 282HelicalSequence analysisAdd BLAST21
    Transmembranei308 – 328HelicalSequence analysisAdd BLAST21
    Transmembranei356 – 376HelicalSequence analysisAdd BLAST21
    Transmembranei382 – 402HelicalSequence analysisAdd BLAST21
    Transmembranei411 – 431HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004189981 – 451GABA transporter 1Add BLAST451

    Proteomic databases

    PaxDbiF4HW02

    Expressioni

    Tissue specificityi

    Highly expressed in flowers and at lower levels in roots, leaves and stems.2 Publications

    Inductioni

    By wounding and senescence.1 Publication

    Gene expression databases

    ExpressionAtlasiF4HW02 baseline and differential
    GenevisibleiF4HW02 AT

    Interactioni

    Protein-protein interaction databases

    BioGridi22585, 7 interactors
    STRINGi3702.AT1G08230.2

    Structurei

    3D structure databases

    ProteinModelPortaliF4HW02
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1303 Eukaryota
    COG0814 LUCA
    HOGENOMiHOG000241624
    InParanoidiF4HW02
    OMAiKDYSISG
    OrthoDBiEOG093609Q7

    Family and domain databases

    InterProiView protein in InterPro
    IPR013057 AA_transpt_TM
    PfamiView protein in Pfam
    PF01490 Aa_trans, 1 hit

    Sequencei

    Sequence statusi: Complete.

    F4HW02-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGGEERSGDG EKRGEEVVDA GSLFVLKSKG TWWHCGFHLT TSIVAPALLS
    60 70 80 90 100
    LPYAFKFLGW AAGISCLVGG AAVTFYSYTL LSLTLEHHAS LGNRYLRFRD
    110 120 130 140 150
    MAHHILSPKW GRYYVGPIQM AVCYGVVIAN ALLGGQCLKA MYLVVQPNGE
    160 170 180 190 200
    MKLFEFVIIF GCLLLVLAQF PSFHSLRYIN SLSLLLCLLY SASAAAASIY
    210 220 230 240 250
    IGKEPNAPEK DYTIVGDPET RVFGIFNAMA IIATTYGNGI IPEIQATISA
    260 270 280 290 300
    PVKGKMMKGL CMCYLVVIMT FFTVAITGYW AFGKKANGLI FTNFLNAETN
    310 320 330 340 350
    HYFVPTWFIF LVNLFTVLQL SAVAVVYLQP INDILESVIS DPTKKEFSIR
    360 370 380 390 400
    NVIPRLVVRS LFVVMATIVA AMLPFFGDVN SLLGAFGFIP LDFVLPVVFF
    410 420 430 440 450
    NFTFKPSKKS FIFWINTVIA VVFSCLGVIA MVAAVRQIII DANTYKLFAD

    V
    Length:451
    Mass (Da):49,695
    Last modified:June 28, 2011 - v1
    Checksum:i1DBC49E86E3088C5
    GO

    Sequence cautioni

    The sequence AAF18251 differs from that shown. Reason: Erroneous gene model prediction.Curated
    The sequence EF183286 differs from that shown. Reason: Frameshift at position 286.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC011438 Genomic DNA Translation: AAF18251.1 Sequence problems.
    CP002684 Genomic DNA Translation: AEE28264.1
    EF183286 mRNA No translation available.
    RefSeqiNP_001077487.1, NM_001084018.3
    UniGeneiAt.42270
    At.73273
    At.75057

    Genome annotation databases

    EnsemblPlantsiAT1G08230.2; AT1G08230.2; AT1G08230
    GeneIDi837344
    GrameneiAT1G08230.2; AT1G08230.2; AT1G08230
    KEGGiath:AT1G08230

    Similar proteinsi

    Entry informationi

    Entry nameiGAT1_ARATH
    AccessioniPrimary (citable) accession number: F4HW02
    Secondary accession number(s): Q9SGD7
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: June 28, 2011
    Last modified: April 25, 2018
    This is version 45 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health