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Protein

GABA transporter 1

Gene

GAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High affinity gamma-aminobutyric acid (GABA) transporter probably involved in GABA uptake into cells. When expressed in a heterologous system (Xenopus oocytes), imports GABA, butylamine, beta- and L-Alanine, 5-aminovaleric acid, 6-aminocaproic acid and 8-aminocaprylic acid, but does not mediate the transport of proline or glycine betaine.2 Publications

Kineticsi

  1. KM=10 µM for GABA1 Publication

pH dependencei

Optimum pH is 4.5-5.5.1 Publication

GO - Molecular functioni

  1. gamma-aminobutyric acid transmembrane transporter activity Source: TAIR

GO - Biological processi

  1. amino acid transmembrane transport Source: GO_Central
  2. gamma-aminobutyric acid transport Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
GABA transporter 1
Short name:
AtGAT1
Alternative name(s):
Bidirectional amino acid transporter 1
Gene namesi
Name:GAT1
Synonyms:BAT1
Ordered Locus Names:At1g08230
ORF Names:T23G18.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G08230.

Subcellular locationi

Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
Note: Plasma membrane.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei35 – 5521HelicalSequence AnalysisAdd
BLAST
Transmembranei57 – 7721HelicalSequence AnalysisAdd
BLAST
Transmembranei115 – 13521HelicalSequence AnalysisAdd
BLAST
Transmembranei153 – 17321HelicalSequence AnalysisAdd
BLAST
Transmembranei182 – 20221HelicalSequence AnalysisAdd
BLAST
Transmembranei222 – 24221HelicalSequence AnalysisAdd
BLAST
Transmembranei262 – 28221HelicalSequence AnalysisAdd
BLAST
Transmembranei308 – 32821HelicalSequence AnalysisAdd
BLAST
Transmembranei356 – 37621HelicalSequence AnalysisAdd
BLAST
Transmembranei382 – 40221HelicalSequence AnalysisAdd
BLAST
Transmembranei411 – 43121HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451GABA transporter 1PRO_0000418998Add
BLAST

Proteomic databases

PRIDEiF4HW02.

Expressioni

Tissue specificityi

Highly expressed in flowers and at lower levels in roots, leaves and stems.2 Publications

Inductioni

By wounding and senescence.1 Publication

Gene expression databases

ExpressionAtlasiF4HW02. baseline and differential.

Structurei

3D structure databases

ProteinModelPortaliF4HW02.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiF4HW02.
OMAiKDYSISG.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4HW02-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGEERSGDG EKRGEEVVDA GSLFVLKSKG TWWHCGFHLT TSIVAPALLS
60 70 80 90 100
LPYAFKFLGW AAGISCLVGG AAVTFYSYTL LSLTLEHHAS LGNRYLRFRD
110 120 130 140 150
MAHHILSPKW GRYYVGPIQM AVCYGVVIAN ALLGGQCLKA MYLVVQPNGE
160 170 180 190 200
MKLFEFVIIF GCLLLVLAQF PSFHSLRYIN SLSLLLCLLY SASAAAASIY
210 220 230 240 250
IGKEPNAPEK DYTIVGDPET RVFGIFNAMA IIATTYGNGI IPEIQATISA
260 270 280 290 300
PVKGKMMKGL CMCYLVVIMT FFTVAITGYW AFGKKANGLI FTNFLNAETN
310 320 330 340 350
HYFVPTWFIF LVNLFTVLQL SAVAVVYLQP INDILESVIS DPTKKEFSIR
360 370 380 390 400
NVIPRLVVRS LFVVMATIVA AMLPFFGDVN SLLGAFGFIP LDFVLPVVFF
410 420 430 440 450
NFTFKPSKKS FIFWINTVIA VVFSCLGVIA MVAAVRQIII DANTYKLFAD

V
Length:451
Mass (Da):49,695
Last modified:June 28, 2011 - v1
Checksum:i1DBC49E86E3088C5
GO

Sequence cautioni

The sequence AAF18251.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence EF183286 differs from that shown. Reason: Frameshift at position 286. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011438 Genomic DNA. Translation: AAF18251.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28264.1.
EF183286 mRNA. No translation available.
RefSeqiNP_001077487.1. NM_001084018.2.
UniGeneiAt.42270.
At.73273.
At.75057.

Genome annotation databases

EnsemblPlantsiAT1G08230.2; AT1G08230.2; AT1G08230.
GeneIDi837344.
KEGGiath:AT1G08230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC011438 Genomic DNA. Translation: AAF18251.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28264.1.
EF183286 mRNA. No translation available.
RefSeqiNP_001077487.1. NM_001084018.2.
UniGeneiAt.42270.
At.73273.
At.75057.

3D structure databases

ProteinModelPortaliF4HW02.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiF4HW02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G08230.2; AT1G08230.2; AT1G08230.
GeneIDi837344.
KEGGiath:AT1G08230.

Organism-specific databases

TAIRiAT1G08230.

Phylogenomic databases

InParanoidiF4HW02.
OMAiKDYSISG.

Gene expression databases

ExpressionAtlasiF4HW02. baseline and differential.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Experimental validation of novel genes predicted in the un-annotated regions of the Arabidopsis genome."
    Moskal W.A. Jr., Wu H.C., Underwood B.A., Wang W., Town C.D., Xiao Y.-L.
    BMC Genomics 8:18-18(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "AtGAT1, a high affinity transporter for gamma-aminobutyric acid in Arabidopsis thaliana."
    Meyer A., Eskandari S., Grallath S., Rentsch D.
    J. Biol. Chem. 281:7197-7204(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  5. "BAT1, a bidirectional amino acid transporter in Arabidopsis."
    Duendar E., Bush D.R.
    Planta 229:1047-1056(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGAT1_ARATH
AccessioniPrimary (citable) accession number: F4HW02
Secondary accession number(s): Q9SGD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: June 28, 2011
Last modified: February 4, 2015
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.