SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

F4HW02

- GAT1_ARATH

UniProt

F4HW02 - GAT1_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
GABA transporter 1
Gene
GAT1, BAT1, At1g08230, T23G18.9
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

High affinity gamma-aminobutyric acid (GABA) transporter probably involved in GABA uptake into cells. When expressed in a heterologous system (Xenopus oocytes), imports GABA, butylamine, beta- and L-Alanine, 5-aminovaleric acid, 6-aminocaproic acid and 8-aminocaprylic acid, but does not mediate the transport of proline or glycine betaine.2 Publications

Kineticsi

  1. KM=10 µM for GABA1 Publication

pH dependencei

Optimum pH is 4.5-5.5.

GO - Molecular functioni

  1. gamma-aminobutyric acid transmembrane transporter activity Source: TAIR
Complete GO annotation...

GO - Biological processi

  1. gamma-aminobutyric acid transport Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
GABA transporter 1
Short name:
AtGAT1
Alternative name(s):
Bidirectional amino acid transporter 1
Gene namesi
Name:GAT1
Synonyms:BAT1
Ordered Locus Names:At1g08230
ORF Names:T23G18.9
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G08230.

Subcellular locationi

Cell membrane; Multi-pass membrane protein Inferred
Note: Plasma membrane.1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei35 – 5521Helical; Reviewed prediction
Add
BLAST
Transmembranei57 – 7721Helical; Reviewed prediction
Add
BLAST
Transmembranei115 – 13521Helical; Reviewed prediction
Add
BLAST
Transmembranei153 – 17321Helical; Reviewed prediction
Add
BLAST
Transmembranei182 – 20221Helical; Reviewed prediction
Add
BLAST
Transmembranei222 – 24221Helical; Reviewed prediction
Add
BLAST
Transmembranei262 – 28221Helical; Reviewed prediction
Add
BLAST
Transmembranei308 – 32821Helical; Reviewed prediction
Add
BLAST
Transmembranei356 – 37621Helical; Reviewed prediction
Add
BLAST
Transmembranei382 – 40221Helical; Reviewed prediction
Add
BLAST
Transmembranei411 – 43121Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 451451GABA transporter 1
PRO_0000418998Add
BLAST

Proteomic databases

PRIDEiF4HW02.

Expressioni

Tissue specificityi

Highly expressed in flowers and at lower levels in roots, leaves and stems.2 Publications

Inductioni

By wounding and senescence.1 Publication

Structurei

3D structure databases

ProteinModelPortaliF4HW02.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OMAiGQSMKAI.

Family and domain databases

InterProiIPR013057. AA_transpt_TM.
[Graphical view]
PfamiPF01490. Aa_trans. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4HW02-1 [UniParc]FASTAAdd to Basket

« Hide

MGGEERSGDG EKRGEEVVDA GSLFVLKSKG TWWHCGFHLT TSIVAPALLS    50
LPYAFKFLGW AAGISCLVGG AAVTFYSYTL LSLTLEHHAS LGNRYLRFRD 100
MAHHILSPKW GRYYVGPIQM AVCYGVVIAN ALLGGQCLKA MYLVVQPNGE 150
MKLFEFVIIF GCLLLVLAQF PSFHSLRYIN SLSLLLCLLY SASAAAASIY 200
IGKEPNAPEK DYTIVGDPET RVFGIFNAMA IIATTYGNGI IPEIQATISA 250
PVKGKMMKGL CMCYLVVIMT FFTVAITGYW AFGKKANGLI FTNFLNAETN 300
HYFVPTWFIF LVNLFTVLQL SAVAVVYLQP INDILESVIS DPTKKEFSIR 350
NVIPRLVVRS LFVVMATIVA AMLPFFGDVN SLLGAFGFIP LDFVLPVVFF 400
NFTFKPSKKS FIFWINTVIA VVFSCLGVIA MVAAVRQIII DANTYKLFAD 450
V 451
Length:451
Mass (Da):49,695
Last modified:June 28, 2011 - v1
Checksum:i1DBC49E86E3088C5
GO

Sequence cautioni

The sequence EF183286 differs from that shown. Reason: Frameshift at position 286.
The sequence AAF18251.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC011438 Genomic DNA. Translation: AAF18251.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28264.1.
EF183286 mRNA. No translation available.
RefSeqiNP_001077487.1. NM_001084018.2.
UniGeneiAt.42270.
At.73273.
At.75057.

Genome annotation databases

EnsemblPlantsiAT1G08230.2; AT1G08230.2; AT1G08230.
GeneIDi837344.
KEGGiath:AT1G08230.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC011438 Genomic DNA. Translation: AAF18251.1 . Sequence problems.
CP002684 Genomic DNA. Translation: AEE28264.1 .
EF183286 mRNA. No translation available.
RefSeqi NP_001077487.1. NM_001084018.2.
UniGenei At.42270.
At.73273.
At.75057.

3D structure databases

ProteinModelPortali F4HW02.
ModBasei Search...

Proteomic databases

PRIDEi F4HW02.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G08230.2 ; AT1G08230.2 ; AT1G08230 .
GeneIDi 837344.
KEGGi ath:AT1G08230.

Organism-specific databases

TAIRi AT1G08230.

Phylogenomic databases

OMAi GQSMKAI.

Family and domain databases

InterProi IPR013057. AA_transpt_TM.
[Graphical view ]
Pfami PF01490. Aa_trans. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Experimental validation of novel genes predicted in the un-annotated regions of the Arabidopsis genome."
    Moskal W.A. Jr., Wu H.C., Underwood B.A., Wang W., Town C.D., Xiao Y.-L.
    BMC Genomics 8:18-18(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "AtGAT1, a high affinity transporter for gamma-aminobutyric acid in Arabidopsis thaliana."
    Meyer A., Eskandari S., Grallath S., Rentsch D.
    J. Biol. Chem. 281:7197-7204(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  5. "BAT1, a bidirectional amino acid transporter in Arabidopsis."
    Duendar E., Bush D.R.
    Planta 229:1047-1056(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiGAT1_ARATH
AccessioniPrimary (citable) accession number: F4HW02
Secondary accession number(s): Q9SGD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: June 28, 2011
Last modified: May 14, 2014
This is version 25 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi