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Protein

GABA transporter 1

Gene

GAT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

High affinity gamma-aminobutyric acid (GABA) transporter probably involved in GABA uptake into cells. When expressed in a heterologous system (Xenopus oocytes), imports GABA, butylamine, beta- and L-Alanine, 5-aminovaleric acid, 6-aminocaproic acid and 8-aminocaprylic acid, but does not mediate the transport of proline or glycine betaine.2 Publications

Kineticsi

  1. KM=10 µM for GABA1 Publication

    pH dependencei

    Optimum pH is 4.5-5.5.1 Publication

    GO - Molecular functioni

    • gamma-aminobutyric acid transmembrane transporter activity Source: TAIR

    GO - Biological processi

    • amino acid transmembrane transport Source: GO_Central
    • gamma-aminobutyric acid transport Source: TAIR
    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GABA transporter 1
    Short name:
    AtGAT1
    Alternative name(s):
    Bidirectional amino acid transporter 1
    Gene namesi
    Name:GAT1
    Synonyms:BAT1
    Ordered Locus Names:At1g08230
    ORF Names:T23G18.9
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548 Componenti: Chromosome 1

    Organism-specific databases

    TAIRiAT1G08230.

    Subcellular locationi

    • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

    • Note: Plasma membrane.

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei35 – 5521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei57 – 7721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei115 – 13521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei153 – 17321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei182 – 20221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei222 – 24221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei262 – 28221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei308 – 32821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei356 – 37621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei382 – 40221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei411 – 43121HelicalSequence AnalysisAdd
    BLAST

    GO - Cellular componenti

    • integral component of membrane Source: UniProtKB-KW
    • plasma membrane Source: TAIR
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 451451GABA transporter 1PRO_0000418998Add
    BLAST

    Proteomic databases

    PRIDEiF4HW02.

    Expressioni

    Tissue specificityi

    Highly expressed in flowers and at lower levels in roots, leaves and stems.2 Publications

    Inductioni

    By wounding and senescence.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliF4HW02.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    HOGENOMiHOG000241624.
    InParanoidiF4HW02.
    OMAiKDYSISG.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    F4HW02-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGGEERSGDG EKRGEEVVDA GSLFVLKSKG TWWHCGFHLT TSIVAPALLS
    60 70 80 90 100
    LPYAFKFLGW AAGISCLVGG AAVTFYSYTL LSLTLEHHAS LGNRYLRFRD
    110 120 130 140 150
    MAHHILSPKW GRYYVGPIQM AVCYGVVIAN ALLGGQCLKA MYLVVQPNGE
    160 170 180 190 200
    MKLFEFVIIF GCLLLVLAQF PSFHSLRYIN SLSLLLCLLY SASAAAASIY
    210 220 230 240 250
    IGKEPNAPEK DYTIVGDPET RVFGIFNAMA IIATTYGNGI IPEIQATISA
    260 270 280 290 300
    PVKGKMMKGL CMCYLVVIMT FFTVAITGYW AFGKKANGLI FTNFLNAETN
    310 320 330 340 350
    HYFVPTWFIF LVNLFTVLQL SAVAVVYLQP INDILESVIS DPTKKEFSIR
    360 370 380 390 400
    NVIPRLVVRS LFVVMATIVA AMLPFFGDVN SLLGAFGFIP LDFVLPVVFF
    410 420 430 440 450
    NFTFKPSKKS FIFWINTVIA VVFSCLGVIA MVAAVRQIII DANTYKLFAD

    V
    Length:451
    Mass (Da):49,695
    Last modified:June 28, 2011 - v1
    Checksum:i1DBC49E86E3088C5
    GO

    Sequence cautioni

    The sequence AAF18251.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
    The sequence EF183286 differs from that shown. Reason: Frameshift at position 286. Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC011438 Genomic DNA. Translation: AAF18251.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28264.1.
    EF183286 mRNA. No translation available.
    RefSeqiNP_001077487.1. NM_001084018.2.
    UniGeneiAt.42270.
    At.73273.
    At.75057.

    Genome annotation databases

    EnsemblPlantsiAT1G08230.2; AT1G08230.2; AT1G08230.
    GeneIDi837344.
    KEGGiath:AT1G08230.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AC011438 Genomic DNA. Translation: AAF18251.1. Sequence problems.
    CP002684 Genomic DNA. Translation: AEE28264.1.
    EF183286 mRNA. No translation available.
    RefSeqiNP_001077487.1. NM_001084018.2.
    UniGeneiAt.42270.
    At.73273.
    At.75057.

    3D structure databases

    ProteinModelPortaliF4HW02.
    ModBaseiSearch...
    MobiDBiSearch...

    Proteomic databases

    PRIDEiF4HW02.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblPlantsiAT1G08230.2; AT1G08230.2; AT1G08230.
    GeneIDi837344.
    KEGGiath:AT1G08230.

    Organism-specific databases

    TAIRiAT1G08230.

    Phylogenomic databases

    HOGENOMiHOG000241624.
    InParanoidiF4HW02.
    OMAiKDYSISG.

    Miscellaneous databases

    PROiF4HW02.

    Family and domain databases

    InterProiIPR013057. AA_transpt_TM.
    [Graphical view]
    PfamiPF01490. Aa_trans. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Experimental validation of novel genes predicted in the un-annotated regions of the Arabidopsis genome."
      Moskal W.A. Jr., Wu H.C., Underwood B.A., Wang W., Town C.D., Xiao Y.-L.
      BMC Genomics 8:18-18(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "AtGAT1, a high affinity transporter for gamma-aminobutyric acid in Arabidopsis thaliana."
      Meyer A., Eskandari S., Grallath S., Rentsch D.
      J. Biol. Chem. 281:7197-7204(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
    5. "BAT1, a bidirectional amino acid transporter in Arabidopsis."
      Duendar E., Bush D.R.
      Planta 229:1047-1056(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiGAT1_ARATH
    AccessioniPrimary (citable) accession number: F4HW02
    Secondary accession number(s): Q9SGD7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 5, 2012
    Last sequence update: June 28, 2011
    Last modified: May 27, 2015
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.