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Protein

Cathepsin B-like protease 1

Gene

CATHB1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Thiol protease that plays a central role in plant programmed cell death (PCD). In addition to its role in protein degradation, may cleave and/or degrade a number of target proteins, activating signaling towards PCD. Contributes to the increase of caspase-3-like activity after UV-C-induced PCD and is required for abiotic stress-induced PCD (PubMed:27058316). Functions redundantly with CATHB2 and CATHB3 in basal defense and distinct forms of plant programmed cell death (PCD). Participates to the establishment of basal resistance against the bacterial pathogen Pseudomonase syringae pv. tomato DC3000. Required for full levels of PCD during resistance (R) gene-mediated hypersensitive response (HR). Involved in the regulation of senescence, a developmental form of PCD in plants (PubMed:19453434).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei151By similarity1
Active sitei306PROSITE-ProRule annotation1
Active sitei327By similarity1

GO - Molecular functioni

  • cysteine-type endopeptidase activity Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processPlant defense

Enzyme and pathway databases

ReactomeiR-ATH-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin B-like protease 1Curated (EC:3.4.22.-Curated)
Alternative name(s):
Cathepsin B1Curated
Short name:
AtCathB11 Publication
Gene namesi
Name:CATHB11 Publication
Ordered Locus Names:At1g02300Imported
ORF Names:T7I23.12Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G02300.
TAIRilocus:2204873. AT1G02300.

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 30Sequence analysisAdd BLAST30
PropeptideiPRO_000043941631 – 102Activation peptideCuratedAdd BLAST72
ChainiPRO_5009031914103 – 362Cathepsin B-like protease 1Add BLAST260
PropeptideiPRO_0000439417363 – 379CuratedAdd BLAST17

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi32N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi69N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi116 ↔ 165By similarity
Disulfide bondi148 ↔ 191By similarity
Glycosylationi171N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi182 ↔ 236By similarity
Disulfide bondi183 ↔ 187By similarity
Disulfide bondi213 ↔ 240By similarity
Disulfide bondi222 ↔ 227By similarity
Glycosylationi330N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiF4HVZ1.

PTM databases

iPTMnetiF4HVZ1.

Expressioni

Inductioni

By dark-induced senescence. Down-regulated by infection with an avirulent strain of the bacterial pathogen Pseudomonase syringae pv. tomato DC3000.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G02300.1.

Structurei

3D structure databases

ProteinModelPortaliF4HVZ1.
SMRiF4HVZ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
InParanoidiF4HVZ1.
KOiK01363.
OMAiLAPCGET.
OrthoDBiEOG09360E2K.

Family and domain databases

InterProiView protein in InterPro
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR012599. Propeptide_C1A.
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiView protein in Pfam
PF00112. Peptidase_C1. 1 hit.
PF08127. Propeptide_C1. 1 hit.
PRINTSiPR00705. PAPAIN.
SMARTiView protein in SMART
SM00645. Pept_C1. 1 hit.
PROSITEiView protein in PROSITE
PS00639. THIOL_PROTEASE_HIS. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F4HVZ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSCCIRLH LLASVFLLLF SSFNLQGIAA ENLSKQKLTS LILQNEIVKE
60 70 80 90 100
VNENPNAGWK AAFNDRFANA TVAEFKRLLG VIQTPKTAYL GVPIVRHDLS
110 120 130 140 150
LKLPKEFDAR TAWSHCTSIR RILVGYILNN VLLWSTITLW FWFLLGHCGS
160 170 180 190 200
CWAFGAVESL SDRFCIKYNL NVSLSANDVI ACCGLLCGFG CNGGFPMGAW
210 220 230 240 250
LYFKYHGVVT QECDPYFDNT GCSHPGCEPT YPTPKCERKC VSRNQLWGES
260 270 280 290 300
KHYGVGAYRI NPDPQDIMAE VYKNGPVEVA FTVYEDFAHY KSGVYKYITG
310 320 330 340 350
TKIGGHAVKL IGWGTSDDGE DYWLLANQWN RSWGDDGYFK IRRGTNECGI
360 370
EQSVVAGLPS EKNVFKGITT SDDLLVSSV
Length:379
Mass (Da):42,263
Last modified:June 28, 2011 - v1
Checksum:iCD76CF08426CD949
GO

Sequence cautioni

The sequence AAC24376 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U89959 Genomic DNA. Translation: AAC24376.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE27412.1.
RefSeqiNP_563647.1. NM_100110.3.
UniGeneiAt.43952.

Genome annotation databases

EnsemblPlantsiAT1G02300.1; AT1G02300.1; AT1G02300.
GeneIDi839576.
GrameneiAT1G02300.1; AT1G02300.1; AT1G02300.
KEGGiath:AT1G02300.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiCATB1_ARATH
AccessioniPrimary (citable) accession number: F4HVZ1
Secondary accession number(s): O23681
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2017
Last sequence update: June 28, 2011
Last modified: June 7, 2017
This is version 53 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families