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Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Pyrococcus sp. (strain NA2)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei153Proton acceptorUniRule annotation1
Binding sitei155SubstrateUniRule annotation1
Metal bindingi179Magnesium; via carbamate groupUniRule annotation1
Metal bindingi181MagnesiumUniRule annotation1
Metal bindingi182MagnesiumUniRule annotation1
Active sitei271Proton acceptorUniRule annotation1
Binding sitei272SubstrateUniRule annotation1
Binding sitei304SubstrateUniRule annotation1
Sitei311Transition state stabilizerUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:PNA2_1620Imported
OrganismiPyrococcus sp. (strain NA2)Imported
Taxonomic identifieri342949 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000008293 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei179N6-carboxylysineUniRule annotation1

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Protein-protein interaction databases

STRINGi342949.PNA2_1620.

Structurei

3D structure databases

ProteinModelPortaliF4HJW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 122RuBisCO_large_NInterPro annotationAdd BLAST117
Domaini132 – 412RuBisCO_largeInterPro annotationAdd BLAST281

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni341 – 343Substrate bindingUniRule annotation3
Regioni363 – 366Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

F4HJW7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEWYLDFVDL DYTPGRDELI VEYYFEPNGV SPEEAAGRIA SESSIGTWTT
60 70 80 90 100
LWKLPEMAKR SIAKVFYLEK HGEGYIAKIA YPLTLFEEGS LVQLFSAIAG
110 120 130 140 150
NVFGMKALKN LRLLDFHPPY EYLRHFKGPQ FGVHGIREFM GVKDRPLTAT
160 170 180 190 200
VPKPKMGWSV EEYAEIAYEL WSGGIDLLKD DENFTSFPFN RFEERVRKLY
210 220 230 240 250
RVRDKVEEET GETKEYLINI TGPVHVMEKR AELVACEGGR YVMIDIVVVG
260 270 280 290 300
WSALQYMREI TEDLGLAIHA HRAMHAAFTR NPRHGITMLA LAKAARMIGV
310 320 330 340 350
DQIHTGTAVG KMAGDYEEIK RINDFLLSKW EHIRPVFPVA SGGLHPGLMP
360 370 380 390 400
ELIRLFGKDL VIQAGGGVMG HPDGPRAGAK ALRDAIEAAV EGVDLEEKAK
410
SSPELKKALE KWGYLKPK
Length:418
Mass (Da):47,086
Last modified:June 28, 2011 - v1
Checksum:iF355A0BB4552D10E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002670 Genomic DNA. Translation: AEC52535.1.

Genome annotation databases

EnsemblBacteriaiAEC52535; AEC52535; PNA2_1620.
KEGGipyn:PNA2_1620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002670 Genomic DNA. Translation: AEC52535.1.

3D structure databases

ProteinModelPortaliF4HJW7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi342949.PNA2_1620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEC52535; AEC52535; PNA2_1620.
KEGGipyn:PNA2_1620.

Phylogenomic databases

eggNOGiarCOG04443. Archaea.
COG1850. LUCA.
KOiK01601.
OMAiFTQDWAS.

Family and domain databases

CDDicd08213. RuBisCO_large_III. 1 hit.
Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3. 1 hit.
InterProiIPR033966. RuBisCO.
IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiF4HJW7_PYRSN
AccessioniPrimary (citable) accession number: F4HJW7
Entry historyi
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 30, 2016
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.