Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

F4HJW7

- F4HJW7_PYRSN

UniProt

F4HJW7 - F4HJW7_PYRSN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Pyrococcus sp. (strain NA2)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

Cofactori

Note: Binds 1 magnesium ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei153 – 1531Proton acceptorUniRule annotation
Binding sitei155 – 1551SubstrateUniRule annotation
Metal bindingi179 – 1791Magnesium; via carbamate groupUniRule annotation
Metal bindingi181 – 1811MagnesiumUniRule annotation
Metal bindingi182 – 1821MagnesiumUniRule annotation
Active sitei271 – 2711Proton acceptorUniRule annotation
Binding sitei272 – 2721SubstrateUniRule annotation
Binding sitei304 – 3041SubstrateUniRule annotation
Sitei311 – 3111Transition state stabilizerUniRule annotation

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. oxidoreductase activity Source: UniProtKB-KW
  3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. AMP catabolic process Source: UniProtKB-HAMAP
  2. carbon fixation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LyaseUniRule annotation, OxidoreductaseUniRule annotation

Keywords - Biological processi

Carbon dioxide fixationUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

BioCyciPSP342949:GI74-1654-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
Short name:
RuBisCOUniRule annotation
Gene namesi
Name:rbcLUniRule annotation
Ordered Locus Names:PNA2_1620Imported
OrganismiPyrococcus sp. (strain NA2)Imported
Taxonomic identifieri342949 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000008293: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei179 – 1791N6-carboxylysineUniRule annotation

Interactioni

Subunit structurei

Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliF4HJW7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni341 – 3433Substrate bindingUniRule annotation
Regioni363 – 3664Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

Phylogenomic databases

KOiK01601.
OMAiFTQDWAS.

Family and domain databases

Gene3Di3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPiMF_01133. RuBisCO_L_type3.
InterProiIPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view]
PfamiPF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view]
SUPFAMiSSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

Sequencei

Sequence statusi: Complete.

F4HJW7-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEWYLDFVDL DYTPGRDELI VEYYFEPNGV SPEEAAGRIA SESSIGTWTT
60 70 80 90 100
LWKLPEMAKR SIAKVFYLEK HGEGYIAKIA YPLTLFEEGS LVQLFSAIAG
110 120 130 140 150
NVFGMKALKN LRLLDFHPPY EYLRHFKGPQ FGVHGIREFM GVKDRPLTAT
160 170 180 190 200
VPKPKMGWSV EEYAEIAYEL WSGGIDLLKD DENFTSFPFN RFEERVRKLY
210 220 230 240 250
RVRDKVEEET GETKEYLINI TGPVHVMEKR AELVACEGGR YVMIDIVVVG
260 270 280 290 300
WSALQYMREI TEDLGLAIHA HRAMHAAFTR NPRHGITMLA LAKAARMIGV
310 320 330 340 350
DQIHTGTAVG KMAGDYEEIK RINDFLLSKW EHIRPVFPVA SGGLHPGLMP
360 370 380 390 400
ELIRLFGKDL VIQAGGGVMG HPDGPRAGAK ALRDAIEAAV EGVDLEEKAK
410
SSPELKKALE KWGYLKPK
Length:418
Mass (Da):47,086
Last modified:June 28, 2011 - v1
Checksum:iF355A0BB4552D10E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002670 Genomic DNA. Translation: AEC52535.1.
RefSeqiWP_013749085.1. NC_015474.1.
YP_004424539.1. NC_015474.1.

Genome annotation databases

EnsemblBacteriaiAEC52535; AEC52535; PNA2_1620.
GeneIDi10555107.
KEGGipyn:PNA2_1620.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002670 Genomic DNA. Translation: AEC52535.1 .
RefSeqi WP_013749085.1. NC_015474.1.
YP_004424539.1. NC_015474.1.

3D structure databases

ProteinModelPortali F4HJW7.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEC52535 ; AEC52535 ; PNA2_1620 .
GeneIDi 10555107.
KEGGi pyn:PNA2_1620.

Phylogenomic databases

KOi K01601.
OMAi FTQDWAS.

Enzyme and pathway databases

BioCyci PSP342949:GI74-1654-MONOMER.

Family and domain databases

Gene3Di 3.20.20.110. 1 hit.
3.30.70.150. 1 hit.
HAMAPi MF_01133. RuBisCO_L_type3.
InterProi IPR017712. RuBisCO_III.
IPR000685. RuBisCO_lsu_C.
IPR017443. RuBisCO_lsu_fd_N.
IPR017444. RuBisCO_lsu_N.
[Graphical view ]
Pfami PF00016. RuBisCO_large. 1 hit.
PF02788. RuBisCO_large_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51649. SSF51649. 1 hit.
SSF54966. SSF54966. 1 hit.
TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete genome sequence of hyperthermophilic Pyrococcus sp. strain NA2, isolated from a deep-sea hydrothermal vent area."
    Lee H.S., Bae S.S., Kim M.S., Kwon K.K., Kang S.G., Lee J.H.
    J. Bacteriol. 193:3666-3667(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NA2Imported.

Entry informationi

Entry nameiF4HJW7_PYRSN
AccessioniPrimary (citable) accession number: F4HJW7
Entry historyi
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 26, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Miscellaneous

Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3