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F4HJW7

- F4HJW7_PYRSN

UniProt

F4HJW7 - F4HJW7_PYRSN

Protein

Ribulose bisphosphate carboxylase

Gene

rbcL

Organism
Pyrococcus sp. (strain NA2)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the addition of molecular CO2 and H2O to ribulose 1,5-bisphosphate (RuBP), generating two molecules of 3-phosphoglycerate (3-PGA). Functions in an archaeal AMP degradation pathway, together with AMP phosphorylase and R15P isomerase.UniRule annotation

    Catalytic activityi

    2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.UniRule annotation
    3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.UniRule annotation

    Cofactori

    Binds 1 magnesium ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei153 – 1531Proton acceptorUniRule annotation
    Binding sitei155 – 1551SubstrateUniRule annotation
    Metal bindingi179 – 1791Magnesium; via carbamate groupUniRule annotation
    Metal bindingi181 – 1811MagnesiumUniRule annotation
    Metal bindingi182 – 1821MagnesiumUniRule annotation
    Active sitei271 – 2711Proton acceptorUniRule annotation
    Binding sitei272 – 2721SubstrateUniRule annotation
    Binding sitei304 – 3041SubstrateUniRule annotation
    Sitei311 – 3111Transition state stabilizerUniRule annotation

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. oxidoreductase activity Source: UniProtKB-KW
    3. ribulose-bisphosphate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. AMP catabolic process Source: UniProtKB-HAMAP
    2. carbon fixation Source: UniProtKB-KW

    Keywords - Molecular functioni

    LyaseUniRule annotation, OxidoreductaseUniRule annotation

    Keywords - Biological processi

    Carbon dioxide fixationUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, Metal-bindingUniRule annotation

    Enzyme and pathway databases

    BioCyciPSP342949:GI74-1654-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribulose bisphosphate carboxylaseUniRule annotation (EC:4.1.1.39UniRule annotation)
    Short name:
    RuBisCOUniRule annotation
    Gene namesi
    Name:rbcLUniRule annotation
    Ordered Locus Names:PNA2_1620Imported
    OrganismiPyrococcus sp. (strain NA2)Imported
    Taxonomic identifieri342949 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
    ProteomesiUP000008293: Chromosome

    PTM / Processingi

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei179 – 1791N6-carboxylysineUniRule annotation

    Interactioni

    Subunit structurei

    Homodimer or homodecamer. In contrast to form I RuBisCO, the form III RuBisCO is composed solely of large subunits.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliF4HJW7.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni341 – 3433Substrate bindingUniRule annotation
    Regioni363 – 3664Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the RuBisCO large chain family. Type III subfamily.UniRule annotation

    Phylogenomic databases

    KOiK01601.
    OMAiFTQDWAS.

    Family and domain databases

    Gene3Di3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPiMF_01133. RuBisCO_L_type3.
    InterProiIPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view]
    PfamiPF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsiTIGR03326. rubisco_III. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    F4HJW7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEWYLDFVDL DYTPGRDELI VEYYFEPNGV SPEEAAGRIA SESSIGTWTT    50
    LWKLPEMAKR SIAKVFYLEK HGEGYIAKIA YPLTLFEEGS LVQLFSAIAG 100
    NVFGMKALKN LRLLDFHPPY EYLRHFKGPQ FGVHGIREFM GVKDRPLTAT 150
    VPKPKMGWSV EEYAEIAYEL WSGGIDLLKD DENFTSFPFN RFEERVRKLY 200
    RVRDKVEEET GETKEYLINI TGPVHVMEKR AELVACEGGR YVMIDIVVVG 250
    WSALQYMREI TEDLGLAIHA HRAMHAAFTR NPRHGITMLA LAKAARMIGV 300
    DQIHTGTAVG KMAGDYEEIK RINDFLLSKW EHIRPVFPVA SGGLHPGLMP 350
    ELIRLFGKDL VIQAGGGVMG HPDGPRAGAK ALRDAIEAAV EGVDLEEKAK 400
    SSPELKKALE KWGYLKPK 418
    Length:418
    Mass (Da):47,086
    Last modified:June 28, 2011 - v1
    Checksum:iF355A0BB4552D10E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002670 Genomic DNA. Translation: AEC52535.1.
    RefSeqiWP_013749085.1. NC_015474.1.
    YP_004424539.1. NC_015474.1.

    Genome annotation databases

    EnsemblBacteriaiAEC52535; AEC52535; PNA2_1620.
    GeneIDi10555107.
    KEGGipyn:PNA2_1620.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002670 Genomic DNA. Translation: AEC52535.1 .
    RefSeqi WP_013749085.1. NC_015474.1.
    YP_004424539.1. NC_015474.1.

    3D structure databases

    ProteinModelPortali F4HJW7.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEC52535 ; AEC52535 ; PNA2_1620 .
    GeneIDi 10555107.
    KEGGi pyn:PNA2_1620.

    Phylogenomic databases

    KOi K01601.
    OMAi FTQDWAS.

    Enzyme and pathway databases

    BioCyci PSP342949:GI74-1654-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.110. 1 hit.
    3.30.70.150. 1 hit.
    HAMAPi MF_01133. RuBisCO_L_type3.
    InterProi IPR017712. RuBisCO_III.
    IPR000685. RuBisCO_lsu_C.
    IPR017443. RuBisCO_lsu_fd_N.
    IPR017444. RuBisCO_lsu_N.
    [Graphical view ]
    Pfami PF00016. RuBisCO_large. 1 hit.
    PF02788. RuBisCO_large_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51649. SSF51649. 1 hit.
    SSF54966. SSF54966. 1 hit.
    TIGRFAMsi TIGR03326. rubisco_III. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete genome sequence of hyperthermophilic Pyrococcus sp. strain NA2, isolated from a deep-sea hydrothermal vent area."
      Lee H.S., Bae S.S., Kim M.S., Kwon K.K., Kang S.G., Lee J.H.
      J. Bacteriol. 193:3666-3667(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NA2Imported.

    Entry informationi

    Entry nameiF4HJW7_PYRSN
    AccessioniPrimary (citable) accession number: F4HJW7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Miscellaneous

    Because the Archaea possessing a type III RuBisCO are all anaerobic, it is most likely that only the carboxylase activity of RuBisCO, and not the competitive oxygenase activity (by which RuBP reacts with O2 to form one molecule of 3-phosphoglycerate and one molecule of 2-phosphoglycolate), is biologically relevant in these strains.UniRule annotation

    Keywords - Technical termi

    Complete proteomeImported

    External Data

    Dasty 3