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Protein

Fructose-1,6-bisphosphate aldolase/phosphatase

Gene

fbp

Organism
Pyrococcus sp. (strain NA2)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).UniRule annotation

Catalytic activityi

D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.UniRule annotation
D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei15Proton acceptor; for FBP phosphatase activityUniRule annotation1
Metal bindingi15Magnesium 1UniRule annotation1
Metal bindingi22Magnesium 1; via pros nitrogenUniRule annotation1
Binding sitei22FBP; via tele nitrogenUniRule annotation1
Metal bindingi56Magnesium 1UniRule annotation1
Metal bindingi56Magnesium 2UniRule annotation1
Metal bindingi57Magnesium 2UniRule annotation1
Binding sitei94FBPUniRule annotation1
Metal bindingi98Magnesium 1UniRule annotation1
Metal bindingi135Magnesium 2UniRule annotation1
Binding sitei136FBP/DHAPUniRule annotation1
Active sitei237Proton donor/acceptor; for FBP aldolase activityUniRule annotation1
Active sitei240Schiff-base intermediate with DHAP; for FBP aldolase activityUniRule annotation1
Metal bindingi240Magnesium 3; via carbonyl oxygenUniRule annotation1
Metal bindingi241Magnesium 3UniRule annotation1
Metal bindingi241Magnesium 4UniRule annotation1
Metal bindingi242Magnesium 2UniRule annotation1
Metal bindingi242Magnesium 3UniRule annotation1
Binding sitei274FBP/DHAP; via amide nitrogenUniRule annotation1
Binding sitei295FBP/DHAPUniRule annotation1
Binding sitei357FBPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolaseUniRule annotation, LyaseUniRule annotation
Biological processCarbohydrate metabolismUniRule annotation, GluconeogenesisUniRule annotation
LigandMagnesiumUniRule annotation, Metal-bindingUniRule annotation, Schiff baseUniRule annotation

Enzyme and pathway databases

BioCyciPSP342949:GI74-1418-MONOMER.
UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-1,6-bisphosphate aldolase/phosphataseUniRule annotation (EC:3.1.3.11UniRule annotation, EC:4.1.2.13UniRule annotation)
Short name:
FBP A/PUniRule annotation
Short name:
FBP aldolase/phosphataseUniRule annotation
Gene namesi
Name:fbpUniRule annotation
Ordered Locus Names:PNA2_1387Imported
OrganismiPyrococcus sp. (strain NA2)Imported
Taxonomic identifieri342949 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000008293 Componenti: Chromosome

Interactioni

Subunit structurei

Homooctamer; dimer of tetramers.UniRule annotation

Protein-protein interaction databases

STRINGi342949.PNA2_1387.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni107 – 108FBP bindingUniRule annotation2
Regioni250 – 251FBP binding; shared with dimeric partnerUniRule annotation2

Domaini

Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.UniRule annotation

Sequence similaritiesi

Belongs to the FBP aldolase/phosphatase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04180. Archaea.
COG1980. LUCA.
KOiK01622.
OMAiHLVAGWM.
OrthoDBiPOG093Z0396.

Family and domain databases

HAMAPiMF_02067. FBP_aldolase_phosphatase. 1 hit.
InterProiView protein in InterPro
IPR002803. FBPase_V.
PfamiView protein in Pfam
PF01950. FBPase_3. 1 hit.
PIRSFiPIRSF015647. FBPtase_archl. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014260. FBPase_V. 1 hit.
SUPFAMiSSF111249. SSF111249. 1 hit.

Sequencei

Sequence statusi: Complete.

F4HIK2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVGEKITIS VIKADVGGWP GHSRVHPALI EKAKEVLSEA QKEGTIIDFY
60 70 80 90 100
VTYAGDDLQL IMTHKKGVDS PEVHGLAWEA FKKATEVAKE LALYGAGQDL
110 120 130 140 150
LKDAFSGNVR GLGPSVAEME ITLRKSEPIV TFHLDKTEPG AFNLPIFRMF
160 170 180 190 200
ADPFNTAGLV IDPKMHMGFR FEIWDILEHK RVIMNSPEEM YDILALIGAK
210 220 230 240 250
GRYVIKRVYP KEGHPIPKDE PVAVVSTEKL YEVAGEYVGK DDPVAIVRAQ
260 270 280 290 300
SGLPALGEVL EPFAFPHLVS GWMRGSHNGP LMPVPLKYAT PTRFDGPPRA
310 320 330 340 350
VALGWQISPE GKLIGPVDLF DDPAFDWARQ KALEITDYMR RHGPFEPHRL
360 370
PLEEMEYTTL PGVLEKLKDR FEPIE
Length:375
Mass (Da):41,769
Last modified:June 28, 2011 - v1
Checksum:i360CA0EDAD37DCD4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002670 Genomic DNA. Translation: AEC52302.1.
RefSeqiWP_013748852.1. NC_015474.1.

Genome annotation databases

EnsemblBacteriaiAEC52302; AEC52302; PNA2_1387.
GeneIDi10554871.
KEGGipyn:PNA2_1387.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002670 Genomic DNA. Translation: AEC52302.1.
RefSeqiWP_013748852.1. NC_015474.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi342949.PNA2_1387.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEC52302; AEC52302; PNA2_1387.
GeneIDi10554871.
KEGGipyn:PNA2_1387.

Phylogenomic databases

eggNOGiarCOG04180. Archaea.
COG1980. LUCA.
KOiK01622.
OMAiHLVAGWM.
OrthoDBiPOG093Z0396.

Enzyme and pathway databases

UniPathwayiUPA00138.
BioCyciPSP342949:GI74-1418-MONOMER.

Family and domain databases

HAMAPiMF_02067. FBP_aldolase_phosphatase. 1 hit.
InterProiView protein in InterPro
IPR002803. FBPase_V.
PfamiView protein in Pfam
PF01950. FBPase_3. 1 hit.
PIRSFiPIRSF015647. FBPtase_archl. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD014260. FBPase_V. 1 hit.
SUPFAMiSSF111249. SSF111249. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiF4HIK2_PYRSN
AccessioniPrimary (citable) accession number: F4HIK2
Entry historyiIntegrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: May 10, 2017
This is version 20 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.