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F4H8N1 (F4H8N1_CELFA) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 14. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP-Rule MF_00191
EC=1.17.1.2 HAMAP-Rule MF_00191
Gene names
Name:ispH HAMAP-Rule MF_00191
Ordered Locus Names:Celf_2917
OrganismCellulomonas fimi (strain ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547) [Complete proteome] [HAMAP] EMBL AEE47039.1
Taxonomic identifier590998 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Micrococcineae › Cellulomonadaceae › Cellulomonas ›

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP-Rule MF_00191 SAAS SAAS003451

Catalytic activity

Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP-Rule MF_00191 SAAS SAAS003451

Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP-Rule MF_00191 SAAS SAAS003451

Cofactor

Binds 1 3Fe-4S cluster per subunit By similarity. HAMAP-Rule MF_00191 SAAS SAAS003451

Pathway

Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP-Rule MF_00191 SAAS SAAS003451

Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP-Rule MF_00191 SAAS SAAS003451

Sequence similarities

Belongs to the IspH family. HAMAP-Rule MF_00191

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region236 – 2383Substrate binding By similarity HAMAP-Rule MF_00191

Sites

Metal binding261Iron-sulfur (3Fe-4S) By similarity HAMAP-Rule MF_00191
Metal binding1101Iron-sulfur (3Fe-4S) By similarity HAMAP-Rule MF_00191
Metal binding2081Iron-sulfur (3Fe-4S) By similarity HAMAP-Rule MF_00191
Binding site551Substrate By similarity HAMAP-Rule MF_00191
Binding site881Substrate By similarity HAMAP-Rule MF_00191
Binding site1381Substrate By similarity HAMAP-Rule MF_00191
Binding site1781Substrate By similarity HAMAP-Rule MF_00191
Binding site2811Substrate By similarity HAMAP-Rule MF_00191

Sequences

Sequence LengthMass (Da)Tools
F4H8N1 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: F76AC0B51DADB3B6

FASTA34737,408
        10         20         30         40         50         60 
MTSATDQAPG VPSTKRVLLA APRGYCAGVD RAVVAVEKAL EHYGAPVYVR KEIVHNKYVV 

        70         80         90        100        110        120 
ETLSARGAVF VDETDEVPEG ARVVFSAHGV SPAVRAAATA RSLQTIDATC PLVTKVHKEA 

       130        140        150        160        170        180 
VRFAADDFDI LLIGHDGHEE VEGTQGEAPE HIQVVNSPDA VDGVEVRDPA RVVWISQTTL 

       190        200        210        220        230        240 
SVDETMETVR RLREKFPLLQ DPPSDDICYA TQNRQVAVKK LAPHCDVVIT VGSANSSNSV 

       250        260        270        280        290        300 
RLVEVALDAG ARASYRVDKA AEIDPAWLEG ATTVGVTSGA SVPEILVRDV LELLAQHGFG 

       310        320        330        340 
DVEEVRTATE DLMFSLPREL RADLKASGAT PDRPRREDRR SLDVQPA

« Hide

References

[1]"Complete sequence of Cellulomonas fimi ATCC 484."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Mead D., Brumm P., Woyke T.
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 484 / DSM 20113 / JCM 1341 / NBRC 15513 / NCIMB 8980 / NCTC 7547.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002666 Genomic DNA. Translation: AEE47039.1.
RefSeqYP_004454426.1. NC_015514.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEE47039; AEE47039; Celf_2917.
GeneID10593716.
KEGGcfi:Celf_2917.
PATRIC54616829. VBICelFim18861_2932.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK03527.

Enzyme and pathway databases

BioCycCFIM590998:GJFK-2956-MONOMER.
UniPathwayUPA00056; UER00097.
UPA00059; UER00105.

Family and domain databases

HAMAPMF_00191. IspH.
InterProIPR003451. LytB.
[Graphical view]
PfamPF02401. LYTB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00216. ispH_lytB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameF4H8N1_CELFA
AccessionPrimary (citable) accession number: F4H8N1
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 3, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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