Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

F4GUQ7 (F4GUQ7_PUSST) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP-Rule MF_00172

EC=2.1.1.14 HAMAP-Rule MF_00172
Alternative name(s):
Cobalamin-independent methionine synthase HAMAP-Rule MF_00172
Methionine synthase, vitamin-B12 independent isozyme HAMAP-Rule MF_00172
Gene names
Name:metE HAMAP-Rule MF_00172
Ordered Locus Names:PT7_1349 EMBL AEC19889.1
OrganismPusillimonas sp. (strain T7-7) [Complete proteome] [HAMAP] EMBL AEC19889.1
Taxonomic identifier1007105 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaePusillimonas

Protein attributes

Sequence length764 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP-Rule MF_00172 SAAS SAAS006276

Catalytic activity

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP-Rule MF_00172 SAAS SAAS006276

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00172

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP-Rule MF_00172 SAAS SAAS006276

Sequence similarities

Belongs to the vitamin-B12 independent methionine synthase family. HAMAP-Rule MF_00172

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding6521Zinc By similarity HAMAP-Rule MF_00172
Metal binding6541Zinc By similarity HAMAP-Rule MF_00172
Metal binding7371Zinc By similarity HAMAP-Rule MF_00172

Sequences

Sequence LengthMass (Da)Tools
F4GUQ7 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 3F43BC18C6AFB4E1

FASTA76485,584
        10         20         30         40         50         60 
MTITHNLGFP RIGVRRELKS SLEAYWAGKQ TLPELEATGR ELRARHWRLQ AKSGLQFVPV 

        70         80         90        100        110        120 
GDFAWYDHIL EWTTLLGAVP ARFGQPSDED VGLDTLFRMA RGRAPSGSPA AACEMTKWFD 

       130        140        150        160        170        180 
TNYHYIVPEL VPEQSYRIAR SYLFDQIREA QSLGHKAKPV IPGPLTWLWL GKGDAFAQGA 

       190        200        210        220        230        240 
SDTAKLTLLD NLVPVYVQAL EQIQNLGVEW VQIDEPILGL DLPDAWQQAF QPVYETLAKG 

       250        260        270        280        290        300 
GPRVLLATYF EDLKENVQVL KGLPVDGVHV DLVRAPDQLA RVAAVLDDRQ VLSAGLIDGR 

       310        320        330        340        350        360 
NVWRSDLDKA GDQIRQVAQQ RGDRLWLAPS CSLLHVPVDL DAERDLDAEL RSWLSFATQK 

       370        380        390        400        410        420 
LDELNVLASS LANTVDADTQ EALVQQRVAL INRKNSSRIH NPAVQQRLKA SVSVTRDRAP 

       430        440        450        460        470        480 
FQQRIASQQK KLGLPSYPTT TIGSFPQTSE IRLARRDWKQ GALSDTAYEK AMRAEIEHVI 

       490        500        510        520        530        540 
RFQEKIGLDV LVHGEPERND MVEYFGELLG GFAFTQNGWV QSYGSRCVKP PIIFGDVVRP 

       550        560        570        580        590        600 
AAMTVAWSAY AQSLTEKPVK GMLTGPVTIL QWSFVRDDQP RATTCQQLAL ALRDEVTDLE 

       610        620        630        640        650        660 
AAGITVIQID EPAFREGLPL RRADWRVYLD WAVDCFRLST GGVQDDTQIH THMCYSEFND 

       670        680        690        700        710        720 
IIESIAAMDA DVITIETSRS NMELLGAFED FHYPNDIGPG VYDIHSPNVP QLEWMVDLMK 

       730        740        750        760 
KASGRLPAER LWVNPDCGLK TRGWPETEAA LLAMVKAAET LRYT 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Pusillimonas sp. T7-7, a cold-tolerant diesel oil-degrading bacterium isolated from the Bohai Sea in China."
Cao B., Ma T., Ren Y., Ren Y., Li G., Li P., Guo X., Ding P., Feng L.
J. Bacteriol. 193:4021-4022(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T7-7 EMBL AEC19889.1.
[2]"Genome Announcement Complete Genome Sequence of Pusillimonas sp. T7-7, a novel species isolated from the Bohai Sea, China."
Cao B., Ma T., Li P., Jiang L., Li Y., Guo X., Feng L.
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: T7-7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002663 Genomic DNA. Translation: AEC19889.1.
RefSeqYP_004416513.1. NC_015458.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEC19889; AEC19889; PT7_1349.
GeneID10606267.
KEGGput:PT7_1349.
PATRIC54556965. VBIPusSp189076_1331.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00549.
OMASFPQTSE.

Enzyme and pathway databases

BioCycPSP1007105:GJAF-1371-MONOMER.
UniPathwayUPA00051; UER00082.

Family and domain databases

HAMAPMF_00172. Meth_synth.
InterProIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]
PfamPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetSearch...

Entry information

Entry nameF4GUQ7_PUSST
AccessionPrimary (citable) accession number: F4GUQ7
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: February 19, 2014
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)