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F4GTU5 (F4GTU5_PUSST) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 22. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:PT7_0014 EMBL AEC18554.1
OrganismPusillimonas sp. (strain T7-7) [Complete proteome] [HAMAP] EMBL AEC18554.1
Taxonomic identifier1007105 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaePusillimonas

Protein attributes

Sequence length315 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain104 – 303200ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding130 – 18556ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2571Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2701Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2721Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F4GTU5 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: F2A81F349A7597DE

FASTA31533,647
        10         20         30         40         50         60 
MNGTYGRVGV LYGGRSAERE VSLMSGQGVF EALRSVGVDA HLFDTGRYTL VDLINAGFDR 

        70         80         90        100        110        120 
VFIALHGRYG EDGTLQGALE LMGLPYTGSG TLASSLAMDK IMTKQVWLQQ GLPTPRYAIL 

       130        140        150        160        170        180 
RPDTDLASVA AVLDLPLIIK PPHEGSTLGV TKVDGLDSLR EAYQLAARYD ADVLAEQFIV 

       190        200        210        220        230        240 
GRELTVALLG GGGSARALPV IEIVAPDGNY DYEHKYISND TQYICPAKLD DALSASIRQI 

       250        260        270        280        290        300 
AEQAYVALGC EGWGRADFML DEAGKPWLLE INTSPGMTSH SLVPMAAKAD GMSYAELCLE 

       310 
ILSTASCKVL TPPAA 

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence of Pusillimonas sp. T7-7, a cold-tolerant diesel oil-degrading bacterium isolated from the Bohai Sea in China."
Cao B., Ma T., Ren Y., Ren Y., Li G., Li P., Guo X., Ding P., Feng L.
J. Bacteriol. 193:4021-4022(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: T7-7 EMBL AEC18554.1.
[2]"Genome Announcement Complete Genome Sequence of Pusillimonas sp. T7-7, a novel species isolated from the Bohai Sea, China."
Cao B., Ma T., Li P., Jiang L., Li Y., Guo X., Feng L.
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: T7-7.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002663 Genomic DNA. Translation: AEC18554.1.
RefSeqYP_004415178.1. NC_015458.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEC18554; AEC18554; PT7_0014.
GeneID10605177.
KEGGput:PT7_0014.
PATRIC54554279. VBIPusSp189076_0014.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.
OMAKLAFQYD.

Enzyme and pathway databases

BioCycPSP1007105:GJAF-14-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF4GTU5_PUSST
AccessionPrimary (citable) accession number: F4GTU5
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 11, 2014
This is version 22 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)