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Protein

Alanine racemase

Gene

PT7_0480

Organism
Pusillimonas sp. (strain T7-7)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids.UniRule annotation

Catalytic activityi

L-alanine = D-alanine.UniRule annotationSAAS annotation

Cofactori

pyridoxal 5'-phosphateUniRule annotationSAAS annotation

Pathway:iD-alanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes D-alanine from L-alanine.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Alanine racemase (PT7_3361), Alanine racemase (PT7_0480)
This subpathway is part of the pathway D-alanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-alanine from L-alanine, the pathway D-alanine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei44 – 441Proton acceptor; specific for D-alanineUniRule annotation
Binding sitei139 – 1391SubstrateUniRule annotation
Active sitei271 – 2711Proton acceptor; specific for L-alanineUniRule annotation
Binding sitei319 – 3191Substrate; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

IsomeraseUniRule annotationSAAS annotation

Keywords - Ligandi

Pyridoxal phosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciPSP1007105:GJAF-489-MONOMER.
UniPathwayiUPA00042; UER00497.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemaseUniRule annotationSAAS annotation (EC:5.1.1.1UniRule annotationSAAS annotation)
Gene namesi
Ordered Locus Names:PT7_0480Imported
OrganismiPusillimonas sp. (strain T7-7)Imported
Taxonomic identifieri1007105 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesAlcaligenaceaePusillimonas
ProteomesiUP000008737 Componenti: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441N6-(pyridoxal phosphate)lysineUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi1007105.PT7_0480.

Structurei

3D structure databases

ProteinModelPortaliF4GMB5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.UniRule annotation

Phylogenomic databases

KOiK01775.
OMAiAPITAWM.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4GMB5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRPISATVS VSSLSHNLQR VANGLQQSCN AAKPLRPRVW AVIKANAYGH
60 70 80 90 100
GIDQAVQGFA QADGLAMLDL NEAVRCRELG WTKPILLLEG FFEPGDIAVL
110 120 130 140 150
DEYCLTTSLH CQEQLEMLAA AKPANPLNAF VKLNVGMNRL GFSEYAFPDA
160 170 180 190 200
YRQAQQLQQQ GILASVGKMS HFARADDSPV TTTQQLQVFN RMTQGLPGPF
210 220 230 240 250
SVCNSAASLT AGLWTTVSSD EEQWVRPGIC LYGSSPFEDR LPDALGLRPA
260 270 280 290 300
MTLAAQLISV REIPRGAGVG YGHVFTASDA MRIGIVACGY ADGYPRHAGT
310 320 330 340 350
GTPICVDGVR TRLVGRVSMD MLAVDLTPVP QAGVGSPVVL WGEGGPSVDE
360 370
VAQAAGTIGY ELLCAVAPRV PRVIAV
Length:376
Mass (Da):40,016
Last modified:June 28, 2011 - v1
Checksum:i6BAD2B1C1F0D1321
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002663 Genomic DNA. Translation: AEC19020.1.
RefSeqiWP_013741569.1. NC_015458.1.

Genome annotation databases

EnsemblBacteriaiAEC19020; AEC19020; PT7_0480.
KEGGiput:PT7_0480.
PATRICi54555223. VBIPusSp189076_0475.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002663 Genomic DNA. Translation: AEC19020.1.
RefSeqiWP_013741569.1. NC_015458.1.

3D structure databases

ProteinModelPortaliF4GMB5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi1007105.PT7_0480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEC19020; AEC19020; PT7_0480.
KEGGiput:PT7_0480.
PATRICi54555223. VBIPusSp189076_0475.

Phylogenomic databases

KOiK01775.
OMAiAPITAWM.

Enzyme and pathway databases

UniPathwayiUPA00042; UER00497.
BioCyciPSP1007105:GJAF-489-MONOMER.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete genome sequence of Pusillimonas sp. T7-7, a cold-tolerant diesel oil-degrading bacterium isolated from the Bohai Sea in China."
    Cao B., Ma T., Ren Y., Ren Y., Li G., Li P., Guo X., Ding P., Feng L.
    J. Bacteriol. 193:4021-4022(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: T7-7Imported.
  2. "Genome Announcement Complete Genome Sequence of Pusillimonas sp. T7-7, a novel species isolated from the Bohai Sea, China."
    Cao B., Ma T., Li P., Jiang L., Li Y., Guo X., Feng L.
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: T7-7.

Entry informationi

Entry nameiF4GMB5_PUSST
AccessioniPrimary (citable) accession number: F4GMB5
Entry historyi
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: July 22, 2015
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.