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F4GCB3 (F4GCB3_ALIDK) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydroorotate dehydrogenase (quinone) HAMAP-Rule MF_00225

EC=1.3.5.2 HAMAP-Rule MF_00225
Alternative name(s):
DHOdehase HAMAP-Rule MF_00225
Dihydroorotate oxidase HAMAP-Rule MF_00225
Gene names
Name:pyrD HAMAP-Rule MF_00225
Ordered Locus Names:Alide2_2408 EMBL AEB84772.1
OrganismAlicycliphilus denitrificans (strain DSM 14773 / CIP 107495 / K601) [Complete proteome] [HAMAP] EMBL AEB84772.1
Taxonomic identifier596154 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeAlicycliphilus

Protein attributes

Sequence length348 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dihydroorotate to orotate with quinone as electron acceptor By similarity. HAMAP-Rule MF_00225 SAAS SAAS001295

Catalytic activity

(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP-Rule MF_00225 SAAS SAAS001295

Cofactor

Binds 1 FMN per subunit By similarity. HAMAP-Rule MF_00225 SAAS SAAS001295

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP-Rule MF_00225 SAAS SAAS001295

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00225 SAAS SAAS001295

Subcellular location

Cell membrane; Peripheral membrane protein By similarity HAMAP-Rule MF_00225.

Sequence similarities

Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily. HAMAP-Rule MF_00225

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding65 – 695FMN By similarity HAMAP-Rule MF_00225
Nucleotide binding326 – 3272FMN By similarity HAMAP-Rule MF_00225
Region114 – 1185Substrate binding By similarity HAMAP-Rule MF_00225
Region254 – 2552Substrate binding By similarity HAMAP-Rule MF_00225

Sites

Active site1831Nucleophile By similarity HAMAP-Rule MF_00225
Binding site691Substrate By similarity HAMAP-Rule MF_00225
Binding site891FMN; via amide nitrogen By similarity HAMAP-Rule MF_00225
Binding site1471FMN By similarity HAMAP-Rule MF_00225
Binding site1801FMN By similarity HAMAP-Rule MF_00225
Binding site1801Substrate By similarity HAMAP-Rule MF_00225
Binding site1851Substrate By similarity HAMAP-Rule MF_00225
Binding site2251FMN By similarity HAMAP-Rule MF_00225
Binding site2531FMN; via carbonyl oxygen By similarity HAMAP-Rule MF_00225
Binding site2761FMN; via amide nitrogen By similarity HAMAP-Rule MF_00225
Binding site3051FMN; via amide nitrogen By similarity HAMAP-Rule MF_00225

Sequences

Sequence LengthMass (Da)Tools
F4GCB3 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 843DD28148E780DF

FASTA34836,742
        10         20         30         40         50         60 
MSLLPYAFAR PFLFGMDAEA AHELTMDMLA RGQRTPLQWA WCNETVSDPV QLAGLTFPNR 

        70         80         90        100        110        120 
VGLAAGLDKN ARCIDALAAM GFGFVEVGTV TPKAQPGNPK PRMFRLPEAR ALINRLGFNN 

       130        140        150        160        170        180 
EGLDAFIANV RRSQVRAQGK PLLLGLNIGK NAATPIEQAT GDYLACLEGV YPHADYVTVN 

       190        200        210        220        230        240 
ISSPNTQNLR ALQSDEALDA LLGAIADHRE KLAAAQGRRV PIFVKIAPDL NEAQVAVIAA 

       250        260        270        280        290        300 
TLTRHGMDGV VATNTTIARD AVQGLPHAGE AGGLSGAPVR EASNAVIRQL RAALGPAFPI 

       310        320        330        340 
VGVGGVMSAE DAVGKIRAGA DVVQIYTGLI YEGPALVSRA ARAIRAMR 

« Hide

References

[1]"Complete sequence of chromosome of Alicycliphilus denitrificans K601."
US DOE Joint Genome Institute
Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Peters L., Zeytun A., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Mikhailova N., Pagani I., Oosterkamp M. expand/collapse author list , Pieper D., van Berkel W., Langenhoff A., Smidt H., Stams A., Woyke T.
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14773 / CIP 107495 / K601.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002657 Genomic DNA. Translation: AEB84772.1.
RefSeqYP_004388288.1. NC_015422.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEB84772; AEB84772; Alide2_2408.
GeneID10483455.
KEGGadk:Alide2_2408.
PATRIC54527494. VBIAliDen79014_2432.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00254.

Enzyme and pathway databases

BioCycADEN596154:GHU6-2439-MONOMER.
UniPathwayUPA00070; UER00946.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00225. DHO_dh_type2.
InterProIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
IPR005719. Dihydroorotate_DH_2.
IPR001295. Dihydroorotate_DH_CS.
[Graphical view]
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMsTIGR01036. pyrD_sub2. 1 hit.
PROSITEPS00911. DHODEHASE_1. 1 hit.
PS00912. DHODEHASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF4GCB3_ALIDK
AccessionPrimary (citable) accession number: F4GCB3
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 11, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)