ID   F4G3I1_METCR            Unreviewed;       509 AA.
AC   F4G3I1;
DT   28-JUN-2011, integrated into UniProtKB/TrEMBL.
DT   28-JUN-2011, sequence version 1.
DT   27-MAR-2024, entry version 44.
DE   RecName: Full=Phosphoenolpyruvate carboxylase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPC {ECO:0000256|HAMAP-Rule:MF_01904};
DE            Short=PEPCase {ECO:0000256|HAMAP-Rule:MF_01904};
DE            EC=4.1.1.31 {ECO:0000256|HAMAP-Rule:MF_01904};
GN   Name=ppcA {ECO:0000256|HAMAP-Rule:MF_01904};
GN   OrderedLocusNames=Mcup_1246 {ECO:0000313|EMBL:AEB95351.1};
OS   Metallosphaera cuprina (strain Ar-4).
OC   Archaea; Thermoproteota; Thermoprotei; Sulfolobales; Sulfolobaceae;
OC   Metallosphaera.
OX   NCBI_TaxID=1006006 {ECO:0000313|EMBL:AEB95351.1, ECO:0000313|Proteomes:UP000007812};
RN   [1] {ECO:0000313|EMBL:AEB95351.1, ECO:0000313|Proteomes:UP000007812}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Ar-4 {ECO:0000313|EMBL:AEB95351.1,
RC   ECO:0000313|Proteomes:UP000007812};
RX   PubMed=21551305; DOI=10.1128/JB.05038-11;
RA   Liu L.J., You X.Y., Zheng H., Wang S., Jiang C.Y., Liu S.J.;
RT   "Complete genome sequence of Metallosphaera cuprina, a metal sulfide-
RT   oxidizing archaeon from a hot spring.";
RL   J. Bacteriol. 193:3387-3388(2011).
CC   -!- FUNCTION: Catalyzes the irreversible beta-carboxylation of
CC       phosphoenolpyruvate (PEP) to form oxaloacetate (OAA), a four-carbon
CC       dicarboxylic acid source for the tricarboxylic acid cycle.
CC       {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=oxaloacetate + phosphate = hydrogencarbonate +
CC         phosphoenolpyruvate; Xref=Rhea:RHEA:28370, ChEBI:CHEBI:16452,
CC         ChEBI:CHEBI:17544, ChEBI:CHEBI:43474, ChEBI:CHEBI:58702; EC=4.1.1.31;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01904};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_01904};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|HAMAP-Rule:MF_01904}.
CC   -!- SIMILARITY: Belongs to the PEPCase type 2 family. {ECO:0000256|HAMAP-
CC       Rule:MF_01904}.
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DR   EMBL; CP002656; AEB95351.1; -; Genomic_DNA.
DR   RefSeq; WP_013737849.1; NC_015435.1.
DR   AlphaFoldDB; F4G3I1; -.
DR   STRING; 1006006.Mcup_1246; -.
DR   GeneID; 10493437; -.
DR   KEGG; mcn:Mcup_1246; -.
DR   PATRIC; fig|1006006.8.peg.1242; -.
DR   eggNOG; arCOG04435; Archaea.
DR   HOGENOM; CLU_517433_0_0_2; -.
DR   OrthoDB; 85849at2157; -.
DR   Proteomes; UP000007812; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0008964; F:phosphoenolpyruvate carboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015977; P:carbon fixation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006107; P:oxaloacetate metabolic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:InterPro.
DR   HAMAP; MF_01904; PEPcase_type2; 1.
DR   InterPro; IPR007566; PEP_COase_arc-type.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   NCBIfam; TIGR02751; PEPCase_arch; 1.
DR   Pfam; PF14010; PEPcase_2; 1.
DR   PIRSF; PIRSF006677; UCP006677; 1.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE   3: Inferred from homology;
KW   Carbon dioxide fixation {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Lyase {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Magnesium {ECO:0000256|HAMAP-Rule:MF_01904};
KW   Pyruvate {ECO:0000313|EMBL:AEB95351.1}.
SQ   SEQUENCE   509 AA;  58200 MW;  B70FE5AA32CC11F5 CRC64;
     MRLIPRTMST QHPDNAHLPE WVKDEVIEGD SEVLEAYYAF SNLGVHEVMW DAEGKDVDTH
     VVRKLFSSFS EYFKNNVLGE DLFLTYRVPN PKIEGAERKV FAETMESIPI TFDVAEKFYG
     RKVVPVFEVI LPFTTNAIDV ISVAKYYEKA VAMEENIELY QGVYVKDLVG EIHPKRVEVI
     PLIEDKDSLL NTRSILEGYY DAMKPTYMRL FIARSDPAMN YGMLTAVLLA KLALSTAYKF
     SREKGIQIFP IIGVGSLPFR GHLSPENYQR VIEEYEGVYT YTVQSAFKYD YSEEQAKGAI
     SHINKEEPRE PKVLTDEEEK TIRDIADKYT SSYQPVIEGM ADLINSIALY LPRRRARKLH
     ISLFGYARST GKVILPRAIT FVGSLYSLGL PPEVIGVSVL SDLTEREWKV LEENYKFMKN
     DLQKASEFIN IEAINSLVSH GFLSEDLSRR IDEDIKYLES LGIKVGPRSY ESKKHALLSQ
     LAILSLKEKR LAEVKQYLKE MAEIRRSVG
//