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F4FAI3

- F4FAI3_VERMA

UniProt

F4FAI3 - F4FAI3_VERMA

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Protein

Formamidopyrimidine-DNA glycosylase

Gene
mutM, fpg, VAB18032_11480
Organism
Verrucosispora maris (strain AB-18-032)
Status
Unreviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity.UniRule annotationSAAS annotations

Catalytic activityi

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.UniRule annotationSAAS annotations
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotationSAAS annotations

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotationSAAS annotations

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21Schiff-base intermediate with DNA By similarityUniRule annotation
Active sitei3 – 31Proton donor By similarityUniRule annotation
Active sitei61 – 611Proton donor; for beta-elimination activity By similarityUniRule annotation
Binding sitei95 – 951DNA By similarityUniRule annotation
Binding sitei115 – 1151DNA By similarityUniRule annotation
Binding sitei157 – 1571DNA By similarityUniRule annotation
Active sitei267 – 2671Proton donor; for delta-elimination activity By similarityUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri243 – 27735FPG-type By similarityUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. damaged DNA binding Source: InterPro
  2. oxidized purine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP
  3. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. base-excision repair Source: InterPro
  2. nucleotide-excision repair Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

GlycosidaseUniRule annotationSAAS annotationsImported, Hydrolase, LyaseUniRule annotationSAAS annotations

Keywords - Biological processi

DNA damage, DNA repairUniRule annotationSAAS annotations

Keywords - Ligandi

DNA-bindingUniRule annotationSAAS annotations, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciVMAR263358:GI1P-1405-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Formamidopyrimidine-DNA glycosylaseUniRule annotation (EC:3.2.2.23UniRule annotation)
Short name:
Fapy-DNA glycosylaseUniRule annotation
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM
Gene namesi
Name:mutMUniRule annotation
Synonyms:fpgUniRule annotation
Ordered Locus Names:VAB18032_11480Imported
OrganismiVerrucosispora maris (strain AB-18-032)Imported
Taxonomic identifieri263358 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeVerrucosispora
ProteomesiUP000008308: Chromosome

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotationSAAS annotations

Family & Domainsi

Sequence similaritiesi

Belongs to the FPG family.UniRule annotation
Contains 1 FPG-type zinc finger.UniRule annotation
Contains FPG-type zinc finger.SAAS annotations

Keywords - Domaini

Zinc-fingerUniRule annotationSAAS annotations

Phylogenomic databases

KOiK10563.

Family and domain databases

HAMAPiMF_00103. Fapy_DNA_glycosyl.
InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamiPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsiTIGR00577. fpg. 1 hit.
PROSITEiPS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4FAI3-1 [UniParc]FASTAAdd to Basket

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MPELPEVETV RQGLARWVTG RRIESVEVRH PRAVRRHVAG GAHFADVLAG    50
RTVLDVCRRG KYLWLPLDSG DAVIGHLGMS GQLLLQPATA PEEPHLRVRF 100
RFTDDGPELR FVDQRTFGGL SVSEGGATLP AEIAHIARDP LDPQFSDADF 150
VTAMRRRRTE VKRALLDQTL ISGVGNIYAD EALWRAGLHG TRPTDALTGP 200
AVRRLLGHVR DVLTEAITAG GTSFDALYVN VNGQSGYFDR SLNVYGREGQ 250
PCRRCGAPIR REAFMNRSSY SCPRCQPRPR ATLRG 285
Length:285
Mass (Da):31,500
Last modified:June 28, 2011 - v1
Checksum:iAFE08BDFBAA5E285
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002638 Genomic DNA. Translation: AEB43410.1.
RefSeqiYP_004404010.1. NC_015434.1.

Genome annotation databases

EnsemblBacteriaiAEB43410; AEB43410; VAB18032_11480.
GeneIDi10450170.
KEGGivma:VAB18032_11480.
PATRICi54471094. VBIVerMar92278_1441.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP002638 Genomic DNA. Translation: AEB43410.1 .
RefSeqi YP_004404010.1. NC_015434.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEB43410 ; AEB43410 ; VAB18032_11480 .
GeneIDi 10450170.
KEGGi vma:VAB18032_11480.
PATRICi 54471094. VBIVerMar92278_1441.

Phylogenomic databases

KOi K10563.

Enzyme and pathway databases

BioCyci VMAR263358:GI1P-1405-MONOMER.

Family and domain databases

HAMAPi MF_00103. Fapy_DNA_glycosyl.
InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
Pfami PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view ]
SUPFAMi SSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsi TIGR00577. fpg. 1 hit.
PROSITEi PS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the abyssomicin- and proximicin-producing marine actinomycete Verrucosispora maris AB-18-032."
    Roh H., Uguru G.C., Ko H.J., Kim S., Kim B.Y., Goodfellow M., Bull A.T., Kim K.H., Bibb M.J., Choi I.G., Stach J.E.
    J. Bacteriol. 193:3391-3392(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AB-18-032Imported.

Entry informationi

Entry nameiF4FAI3_VERMA
AccessioniPrimary (citable) accession number: F4FAI3
Entry historyi
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 11, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotations

External Data

Dasty 3

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