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Protein

Formamidopyrimidine-DNA glycosylase

Gene

mutM

Organism
Verrucosispora maris (strain AB-18-032)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotationSAAS annotation

Catalytic activityi

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.UniRule annotationSAAS annotation
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotationSAAS annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2Schiff-base intermediate with DNAUniRule annotation1
Active sitei3Proton donorUniRule annotation1
Active sitei61Proton donor; for beta-elimination activityUniRule annotation1
Binding sitei95DNAUniRule annotation1
Binding sitei115DNAUniRule annotation1
Binding sitei157DNAUniRule annotation1
Active sitei267Proton donor; for delta-elimination activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotationSAAS annotation, GlycosidaseUniRule annotationSAAS annotationImported, Hydrolase, LyaseUniRule annotationSAAS annotation
Biological processDNA damage, DNA repairUniRule annotationSAAS annotation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Formamidopyrimidine-DNA glycosylaseUniRule annotation (EC:3.2.2.23UniRule annotation)
Short name:
Fapy-DNA glycosylaseUniRule annotation
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutMUniRule annotation (EC:4.2.99.18UniRule annotation)
Short name:
AP lyase MutMUniRule annotation
Gene namesi
Name:mutMUniRule annotation
Synonyms:fpgUniRule annotation
Ordered Locus Names:VAB18032_11480Imported
OrganismiVerrucosispora maris (strain AB-18-032)Imported
Taxonomic identifieri263358 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaMicromonosporalesMicromonosporaceaeVerrucosispora
Proteomesi
  • UP000008308 Componenti: Chromosome

Interactioni

Subunit structurei

Monomer.UniRule annotationSAAS annotation

Protein-protein interaction databases

STRINGi263358.VAB18032_11480.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 118FPG_CATInterPro annotationAdd BLAST117
Domaini243 – 277FPG-typeInterPro annotationAdd BLAST35

Sequence similaritiesi

Belongs to the FPG family.UniRule annotationSAAS annotation

Keywords - Domaini

Zinc-fingerUniRule annotationSAAS annotation

Phylogenomic databases

eggNOGiENOG4105ERD. Bacteria.
COG0266. LUCA.
KOiK10563.
OMAiGVHLRMT.
OrthoDBiPOG091H01RH.

Family and domain databases

HAMAPiMF_00103. Fapy_DNA_glycosyl. 1 hit.
InterProiView protein in InterPro
IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR012319. FPG_cat.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_FPG/IleRS.
PfamiView protein in Pfam
PF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
SMARTiView protein in SMART
SM00898. Fapy_DNA_glyco. 1 hit.
SM01232. H2TH. 1 hit.
SUPFAMiSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsiTIGR00577. fpg. 1 hit.
PROSITEiView protein in PROSITE
PS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.

Sequencei

Sequence statusi: Complete.

F4FAI3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPELPEVETV RQGLARWVTG RRIESVEVRH PRAVRRHVAG GAHFADVLAG
60 70 80 90 100
RTVLDVCRRG KYLWLPLDSG DAVIGHLGMS GQLLLQPATA PEEPHLRVRF
110 120 130 140 150
RFTDDGPELR FVDQRTFGGL SVSEGGATLP AEIAHIARDP LDPQFSDADF
160 170 180 190 200
VTAMRRRRTE VKRALLDQTL ISGVGNIYAD EALWRAGLHG TRPTDALTGP
210 220 230 240 250
AVRRLLGHVR DVLTEAITAG GTSFDALYVN VNGQSGYFDR SLNVYGREGQ
260 270 280
PCRRCGAPIR REAFMNRSSY SCPRCQPRPR ATLRG
Length:285
Mass (Da):31,500
Last modified:June 28, 2011 - v1
Checksum:iAFE08BDFBAA5E285
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002638 Genomic DNA. Translation: AEB43410.1.
RefSeqiWP_013732080.1. NC_015434.1.

Genome annotation databases

EnsemblBacteriaiAEB43410; AEB43410; VAB18032_11480.
KEGGivma:VAB18032_11480.

Similar proteinsi

Entry informationi

Entry nameiF4FAI3_VERMA
AccessioniPrimary (citable) accession number: F4FAI3
Entry historyiIntegrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: September 27, 2017
This is version 44 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotation, Reference proteomeImported