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F4FAI3

- F4FAI3_VERMA

UniProt

F4FAI3 - F4FAI3_VERMA

Protein

Formamidopyrimidine-DNA glycosylase

Gene

mutM

Organism
Verrucosispora maris (strain AB-18-032)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 19 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.UniRule annotationSAAS annotation

    Catalytic activityi

    Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.UniRule annotationSAAS annotation
    The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotationSAAS annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotationSAAS annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21Schiff-base intermediate with DNAUniRule annotation
    Active sitei3 – 31Proton donorUniRule annotation
    Active sitei61 – 611Proton donor; for beta-elimination activityUniRule annotation
    Binding sitei95 – 951DNAUniRule annotation
    Binding sitei115 – 1151DNAUniRule annotation
    Binding sitei157 – 1571DNAUniRule annotation
    Active sitei267 – 2671Proton donor; for delta-elimination activityUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri243 – 27735FPG-typeUniRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. damaged DNA binding Source: InterPro
    2. oxidized purine nucleobase lesion DNA N-glycosylase activity Source: UniProtKB-HAMAP
    3. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. base-excision repair Source: InterPro
    2. nucleotide-excision repair Source: InterPro

    Keywords - Molecular functioni

    GlycosidaseUniRule annotationSAAS annotationImported, Hydrolase, LyaseUniRule annotationSAAS annotation

    Keywords - Biological processi

    DNA damage, DNA repairUniRule annotationSAAS annotation

    Keywords - Ligandi

    DNA-bindingUniRule annotationSAAS annotation, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciVMAR263358:GI1P-1405-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Formamidopyrimidine-DNA glycosylaseUniRule annotation (EC:3.2.2.23UniRule annotation)
    Short name:
    Fapy-DNA glycosylaseUniRule annotation
    Alternative name(s):
    DNA-(apurinic or apyrimidinic site) lyase MutMUniRule annotation
    Gene namesi
    Name:mutMUniRule annotation
    Synonyms:fpgUniRule annotation
    Ordered Locus Names:VAB18032_11480Imported
    OrganismiVerrucosispora maris (strain AB-18-032)Imported
    Taxonomic identifieri263358 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeVerrucosispora
    ProteomesiUP000008308: Chromosome

    Interactioni

    Subunit structurei

    Monomer.UniRule annotationSAAS annotation

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the FPG family.UniRule annotation
    Contains 1 FPG-type zinc finger.UniRule annotation
    Contains FPG-type zinc finger.SAAS annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri243 – 27735FPG-typeUniRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-fingerUniRule annotationSAAS annotation

    Phylogenomic databases

    KOiK10563.

    Family and domain databases

    HAMAPiMF_00103. Fapy_DNA_glycosyl.
    InterProiIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR000191. DNA_glycosylase/AP_lyase.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR020629. Formamido-pyr_DNA_Glyclase.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PfamiPF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    SMARTiSM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view]
    SUPFAMiSSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    TIGRFAMsiTIGR00577. fpg. 1 hit.
    PROSITEiPS51068. FPG_CAT. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    F4FAI3-1 [UniParc]FASTAAdd to Basket

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    MPELPEVETV RQGLARWVTG RRIESVEVRH PRAVRRHVAG GAHFADVLAG    50
    RTVLDVCRRG KYLWLPLDSG DAVIGHLGMS GQLLLQPATA PEEPHLRVRF 100
    RFTDDGPELR FVDQRTFGGL SVSEGGATLP AEIAHIARDP LDPQFSDADF 150
    VTAMRRRRTE VKRALLDQTL ISGVGNIYAD EALWRAGLHG TRPTDALTGP 200
    AVRRLLGHVR DVLTEAITAG GTSFDALYVN VNGQSGYFDR SLNVYGREGQ 250
    PCRRCGAPIR REAFMNRSSY SCPRCQPRPR ATLRG 285
    Length:285
    Mass (Da):31,500
    Last modified:June 28, 2011 - v1
    Checksum:iAFE08BDFBAA5E285
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002638 Genomic DNA. Translation: AEB43410.1.
    RefSeqiYP_004404010.1. NC_015434.1.

    Genome annotation databases

    EnsemblBacteriaiAEB43410; AEB43410; VAB18032_11480.
    GeneIDi10450170.
    KEGGivma:VAB18032_11480.
    PATRICi54471094. VBIVerMar92278_1441.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002638 Genomic DNA. Translation: AEB43410.1 .
    RefSeqi YP_004404010.1. NC_015434.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AEB43410 ; AEB43410 ; VAB18032_11480 .
    GeneIDi 10450170.
    KEGGi vma:VAB18032_11480.
    PATRICi 54471094. VBIVerMar92278_1441.

    Phylogenomic databases

    KOi K10563.

    Enzyme and pathway databases

    BioCyci VMAR263358:GI1P-1405-MONOMER.

    Family and domain databases

    HAMAPi MF_00103. Fapy_DNA_glycosyl.
    InterProi IPR015886. DNA_glyclase/AP_lyase_DNA-bd.
    IPR000191. DNA_glycosylase/AP_lyase.
    IPR012319. DNA_glycosylase/AP_lyase_cat.
    IPR020629. Formamido-pyr_DNA_Glyclase.
    IPR010979. Ribosomal_S13-like_H2TH.
    IPR000214. Znf_DNA_glyclase/AP_lyase.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    Pfami PF01149. Fapy_DNA_glyco. 1 hit.
    PF06831. H2TH. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    SMARTi SM00898. Fapy_DNA_glyco. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46946. SSF46946. 1 hit.
    SSF81624. SSF81624. 1 hit.
    TIGRFAMsi TIGR00577. fpg. 1 hit.
    PROSITEi PS51068. FPG_CAT. 1 hit.
    PS51066. ZF_FPG_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the abyssomicin- and proximicin-producing marine actinomycete Verrucosispora maris AB-18-032."
      Roh H., Uguru G.C., Ko H.J., Kim S., Kim B.Y., Goodfellow M., Bull A.T., Kim K.H., Bibb M.J., Choi I.G., Stach J.E.
      J. Bacteriol. 193:3391-3392(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AB-18-032Imported.

    Entry informationi

    Entry nameiF4FAI3_VERMA
    AccessioniPrimary (citable) accession number: F4FAI3
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 19 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzymeUniRule annotationSAAS annotation, Reference proteomeImported

    External Data

    Dasty 3