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F4FAI3 (F4FAI3_VERMA) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Formamidopyrimidine-DNA glycosylase HAMAP-Rule MF_00103

Short name=Fapy-DNA glycosylase HAMAP-Rule MF_00103
EC=3.2.2.23 HAMAP-Rule MF_00103
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyase MutM HAMAP-Rule MF_00103
Gene names
Name:mutM HAMAP-Rule MF_00103
Synonyms:fpg HAMAP-Rule MF_00103
Ordered Locus Names:VAB18032_11480 EMBL AEB43410.1
OrganismVerrucosispora maris (strain AB-18-032) [Complete proteome] [HAMAP] EMBL AEB43410.1
Taxonomic identifier263358 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeVerrucosispora

Protein attributes

Sequence length285 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates By similarity. HAMAP-Rule MF_00103 SAAS SAAS000191

Catalytic activity

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine. HAMAP-Rule MF_00103 SAAS SAAS000191

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. HAMAP-Rule MF_00103 SAAS SAAS000191

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_00103 SAAS SAAS000191

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00103 SAAS SAAS000191

Sequence similarities

Belongs to the FPG family. HAMAP-Rule MF_00103

Contains 1 FPG-type zinc finger. HAMAP-Rule MF_00103

Contains FPG-type zinc finger. SAAS SAAS000191

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Zinc finger243 – 27735FPG-type By similarity HAMAP-Rule MF_00103

Sites

Active site21Schiff-base intermediate with DNA By similarity HAMAP-Rule MF_00103
Active site31Proton donor By similarity HAMAP-Rule MF_00103
Active site611Proton donor; for beta-elimination activity By similarity HAMAP-Rule MF_00103
Active site2671Proton donor; for delta-elimination activity By similarity HAMAP-Rule MF_00103
Binding site951DNA By similarity HAMAP-Rule MF_00103
Binding site1151DNA By similarity HAMAP-Rule MF_00103
Binding site1571DNA By similarity HAMAP-Rule MF_00103

Sequences

Sequence LengthMass (Da)Tools
F4FAI3 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: AFE08BDFBAA5E285

FASTA28531,500
        10         20         30         40         50         60 
MPELPEVETV RQGLARWVTG RRIESVEVRH PRAVRRHVAG GAHFADVLAG RTVLDVCRRG 

        70         80         90        100        110        120 
KYLWLPLDSG DAVIGHLGMS GQLLLQPATA PEEPHLRVRF RFTDDGPELR FVDQRTFGGL 

       130        140        150        160        170        180 
SVSEGGATLP AEIAHIARDP LDPQFSDADF VTAMRRRRTE VKRALLDQTL ISGVGNIYAD 

       190        200        210        220        230        240 
EALWRAGLHG TRPTDALTGP AVRRLLGHVR DVLTEAITAG GTSFDALYVN VNGQSGYFDR 

       250        260        270        280 
SLNVYGREGQ PCRRCGAPIR REAFMNRSSY SCPRCQPRPR ATLRG 

« Hide

References

[1]"Genome sequence of the abyssomicin- and proximicin-producing marine actinomycete Verrucosispora maris AB-18-032."
Roh H., Uguru G.C., Ko H.J., Kim S., Kim B.Y., Goodfellow M., Bull A.T., Kim K.H., Bibb M.J., Choi I.G., Stach J.E.
J. Bacteriol. 193:3391-3392(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AB-18-032 EMBL AEB43410.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002638 Genomic DNA. Translation: AEB43410.1.
RefSeqYP_004404010.1. NC_015434.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEB43410; AEB43410; VAB18032_11480.
GeneID10450170.
KEGGvma:VAB18032_11480.
PATRIC54471094. VBIVerMar92278_1441.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK10563.

Enzyme and pathway databases

BioCycVMAR263358:GI1P-1405-MONOMER.

Family and domain databases

HAMAPMF_00103. Fapy_DNA_glycosyl.
InterProIPR015886. DNA_glyclase/AP_lyase_DNA-bd.
IPR000191. DNA_glycosylase/AP_lyase.
IPR012319. DNA_glycosylase/AP_lyase_cat.
IPR020629. Formamido-pyr_DNA_Glyclase.
IPR010979. Ribosomal_S13-like_H2TH.
IPR000214. Znf_DNA_glyclase/AP_lyase.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PfamPF01149. Fapy_DNA_glyco. 1 hit.
PF06831. H2TH. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
SMARTSM00898. Fapy_DNA_glyco. 1 hit.
[Graphical view]
SUPFAMSSF46946. SSF46946. 1 hit.
SSF81624. SSF81624. 1 hit.
TIGRFAMsTIGR00577. fpg. 1 hit.
PROSITEPS51068. FPG_CAT. 1 hit.
PS51066. ZF_FPG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF4FAI3_VERMA
AccessionPrimary (citable) accession number: F4FAI3
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 11, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)