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F4F5K5 (F4F5K5_VERMA) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:VAB18032_09595 EMBL AEB43037.1
OrganismVerrucosispora maris (strain AB-18-032) [Complete proteome] [HAMAP] EMBL AEB43037.1
Taxonomic identifier263358 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeVerrucosispora

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain167 – 371205ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding198 – 25356ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3241Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3381Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3381Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3401Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F4F5K5 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 868B27B093286B07

FASTA38841,114
        10         20         30         40         50         60 
MSERHSGGAR RDGGGRPQVI GVLFGGPSAE HEVSCASALG VARALVGGGY QVVALGVTRQ 

        70         80         90        100        110        120 
GRLRLVPEET LAAHIDGTAA ARAIDDRLSV TGSAVELRAG ERPGTALVTG AESPGEVHAV 

       130        140        150        160        170        180 
LDVVFPVLHG PYGEDGVVQG LLESLGVPYV GCGILASAVG MDKVAMKRAL RAEGVPITPH 

       190        200        210        220        230        240 
VWFDEHTYRT TEDVEKLVVG LRRPLFVKPA RMGSSIGISR VTEDDDLAAA VEEALRHDNL 

       250        260        270        280        290        300 
VVVEQGVTGR ELECGVLGGT RPEASTVGEV RVTGGWFDYQ QKYFGDADPM VVPAVLPDEV 

       310        320        330        340        350        360 
TERIRDLSLR AFAAIGGWGL ARVDFLYDES AGELYVNELN TMPGFTAHSM YPKVWAAAGV 

       370        380 
GYQQIVDRLV ALAVERRASR PTSQEGQA 

« Hide

References

[1]"Genome sequence of the abyssomicin- and proximicin-producing marine actinomycete Verrucosispora maris AB-18-032."
Roh H., Uguru G.C., Ko H.J., Kim S., Kim B.Y., Goodfellow M., Bull A.T., Kim K.H., Bibb M.J., Choi I.G., Stach J.E.
J. Bacteriol. 193:3391-3392(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AB-18-032 EMBL AEB43037.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002638 Genomic DNA. Translation: AEB43037.1.
RefSeqYP_004403637.1. NC_015434.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEB43037; AEB43037; VAB18032_09595.
GeneID10449789.
KEGGvma:VAB18032_09595.
PATRIC54470320. VBIVerMar92278_1066.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycVMAR263358:GI1P-1024-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF4F5K5_VERMA
AccessionPrimary (citable) accession number: F4F5K5
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: February 19, 2014
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)