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F4DS22 (F4DS22_PSEMN) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:MDS_1012 EMBL AEB57043.1
OrganismPseudomonas mendocina (strain NK-01) [Complete proteome] [HAMAP] EMBL AEB57043.1
Taxonomic identifier1001585 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length313 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain111 – 306196ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding137 – 19256ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2601Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2731Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2731Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2751Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F4DS22 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: A72212FB666A3679

FASTA31333,180
        10         20         30         40         50         60 
MSLHSTLDPK AFGRVAVLFG GKSAEREVSL KSGNAVLSAL QAAGVDAFGI DVGDDFLARL 

        70         80         90        100        110        120 
NSEKIDRAFI VLHGRGGEDG SMQGLLECAG IPYTGSGILA SALAMDKLRT KQVWHSLGLP 

       130        140        150        160        170        180 
TPRHAVLTSQ ADCKAAATEL GFPLIVKPAH EGSSIGMAKV ESVEALVAAW QDAARYDSQV 

       190        200        210        220        230        240 
LVEQWIAGPE YTIAVLRGEV LPPIGLGTPH TFYDYDAKYL ADDTQYRIPC GLSAEKEAEL 

       250        260        270        280        290        300 
KSLTARACEA VGTQGWARAD VMQDASGQFW LLEVNTVPGM TDHSLVPMAA RAAGLDFQQL 

       310 
VLAILADSVE ARG 

« Hide

References

[1]"Complete genome of Pseudomonas mendocina NK-01, which synthesizes medium-chain-length polyhydroxyalkanoates and alginate oligosaccharides."
Guo W., Wang Y., Song C., Yang C., Li Q., Li B., Su W., Sun X., Song D., Yang X., Wang S.
J. Bacteriol. 193:3413-3414(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NK-01 EMBL AEB57043.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002620 Genomic DNA. Translation: AEB57043.1.
RefSeqYP_004378795.1. NC_015410.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEB57043; AEB57043; MDS_1012.
GeneID10455794.
KEGGpmk:MDS_1012.
PATRIC54482578. VBIPseMen187712_1031.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.
OMADTQYRIP.

Enzyme and pathway databases

BioCycPMEN1001585:GIWS-1018-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF4DS22_PSEMN
AccessionPrimary (citable) accession number: F4DS22
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: February 19, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)