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F4CD21

- F4CD21_SPHS2

UniProt

F4CD21 - F4CD21_SPHS2

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Sphingobacterium sp. (strain 21)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

    Cofactori

    Magnesium.UniRule annotationSAAS annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141ATP; via amide nitrogenUniRule annotation
    Metal bindingi106 – 1061Magnesium; catalyticUniRule annotation
    Active sitei131 – 1311Proton acceptorUniRule annotation
    Binding sitei158 – 1581Allosteric activator ADPUniRule annotation
    Binding sitei166 – 1661Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei225 – 2251SubstrateUniRule annotation
    Binding sitei249 – 2491Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi75 – 762ATPUniRule annotation
    Nucleotide bindingi105 – 1084ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    KinaseUniRule annotationSAAS annotationImported, Transferase

    Keywords - Biological processi

    GlycolysisUniRule annotationSAAS annotation

    Keywords - Ligandi

    ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciSSP743722:GH04-1077-MONOMER.
    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    Ordered Locus Names:Sph21_1062Imported
    OrganismiSphingobacterium sp. (strain 21)Imported
    Taxonomic identifieri743722 [NCBI]
    Taxonomic lineageiBacteriaBacteroidetesSphingobacteriiaSphingobacterialesSphingobacteriaceaeSphingobacterium
    ProteomesiUP000007808: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotationSAAS annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    CytoplasmUniRule annotationSAAS annotation

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotationSAAS annotation

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni24 – 285Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni129 – 1313Substrate bindingUniRule annotation
    Regioni173 – 1753Substrate bindingUniRule annotation
    Regioni189 – 1913Allosteric activator ADP bindingUniRule annotation
    Regioni216 – 2183Allosteric activator ADP bindingUniRule annotation
    Regioni255 – 2584Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Phylogenomic databases

    KOiK00850.
    OMAiMELREGH.

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    F4CD21-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTIKNIAVF TSGGDSPGMN AAIRAVVRTS LHYNLNVFGI QRGYDGMVNG    50
    DIFPMDAKSV ANIIQRGGTI LKTARSKEFR TVEGRQQAYE QIRKFQIDGL 100
    VAIGGDGTFT GAAKFIEEHD IPVMGLPGTI DNDLAGTDFT IGYDTAINTV 150
    ISAVDKIRDT AESHDRLFII EVMGRDSGLI ALRSGIGTGA EAILIPESAT 200
    NSKALLEKLA HGRKDKSSKI VLVAEGDEEG GAFAIAELVK KHFPNYDTRV 250
    SILGHIQRGG SPTCMDRVLA SRLGVAAVEA LLAGRKGEMA GVINGDIAFT 300
    PFNKAIKHID SLTPSLLKIV DILS 324
    Length:324
    Mass (Da):34,525
    Last modified:June 28, 2011 - v1
    Checksum:iE270CCCE1B5FD1D6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002584 Genomic DNA. Translation: ADZ77632.1.
    RefSeqiWP_013664360.1. NC_015277.1.
    YP_004316302.1. NC_015277.1.

    Genome annotation databases

    EnsemblBacteriaiADZ77632; ADZ77632; Sph21_1062.
    GeneIDi10346804.
    KEGGishg:Sph21_1062.
    PATRICi47235888. VBISphSp165585_1138.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP002584 Genomic DNA. Translation: ADZ77632.1 .
    RefSeqi WP_013664360.1. NC_015277.1.
    YP_004316302.1. NC_015277.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADZ77632 ; ADZ77632 ; Sph21_1062 .
    GeneIDi 10346804.
    KEGGi shg:Sph21_1062.
    PATRICi 47235888. VBISphSp165585_1138.

    Phylogenomic databases

    KOi K00850.
    OMAi MELREGH.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .
    BioCyci SSP743722:GH04-1077-MONOMER.

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Sphingobacterium sp. 21."
      Lucas S., Copeland A., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S., Davenport K., Detter J.C., Han C., Tapia R., Land M., Hauser L., Kyrpides N., Ivanova N., Ovchinnikova G., Pagani I., Siebers A.K., Allgaier M.
      , Thelen M.P., Hugenholtz P., Woyke T.
      Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 21Imported.

    Entry informationi

    Entry nameiF4CD21_SPHS2
    AccessioniPrimary (citable) accession number: F4CD21
    Entry historyi
    Integrated into UniProtKB/TrEMBL: June 28, 2011
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 22 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3