Skip Header

Contribute Send feedback
Read comments (?) or add your own

F4BCW3 (F4BCW3_FRACF) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 15. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:FNFX1_0157 EMBL AEB27105.1
OrganismFrancisella cf. novicida (strain Fx1) [Complete proteome] [HAMAP]
Taxonomic identifier984129 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Thiotrichales › Francisellaceae › Francisella ›

Protein attributes

Sequence length296 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047 SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain103 – 293191ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding129 – 18052ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2471Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2601Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2601Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2621Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F4BCW3 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 8733294194062AA2

FASTA29632,786
        10         20         30         40         50         60 
MKNEKIVVLY GGDSPEREVS LKSGKAVLDS LISQGYDAVG VDASGKELVA KLLELKPDKC 

        70         80         90        100        110        120 
FVALHGEDGE NGRVSALLEM LEIKHTSSSM KSSVITMDKM ISKEILMHHR MPTPMAKFLT 

       130        140        150        160        170        180 
DKLVAEDEIS FPVAVKPSSG GSSIATFKVK SIQELKHAYE EASKYGEVMI EQWVTGKEIT 

       190        200        210        220        230        240 
VAIVNDEVYS SVWIEPQNEF YDYESKYSGK SIYHSPSGLC EQKELEVRQL AKKAYDLLGC 

       250        260        270        280        290 
SGHARVDFIY DDRGNFYIME INSSPGMTEN SLSPKSAAAE GVDFDSFVKR IIEQAQ

« Hide

References

[1]"Common ancestry and novel genetic traits of Francisella novicida-like isolates from North America and Australia as revealed by comparative genomic analyses."
Siddaramappa S., Challacombe J.F., Petersen J.M., Pillai S., Hogg G., Kuske C.R.
Appl. Environ. Microbiol. 77:5110-5122(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Fx1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002557 Genomic DNA. Translation: AEB27105.1.
RefSeqYP_005825065.1. NC_017450.1.

3D structure databases

ProteinModelPortalF4BCW3.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEB27105; AEB27105; FNFX1_0157.
GeneID12434946.
KEGGfcf:FNFX1_0157.
PATRIC54253859. VBIFraCf186347_0155.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF4BCW3_FRACF
AccessionPrimary (citable) accession number: F4BCW3
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 3, 2013
This is version 15 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info