Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

F4BCW3

- F4BCW3_FRACF

UniProt

F4BCW3 - F4BCW3_FRACF

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Francisella cf. novicida (strain Fx1)
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotationSAAS annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotationSAAS annotation

Cofactori

Note: Binds 2 magnesium or manganese ions per subunit.UniRule annotationSAAS annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi247 – 2471Magnesium or manganese 1UniRule annotation
Metal bindingi260 – 2601Magnesium or manganese 1UniRule annotation
Metal bindingi260 – 2601Magnesium or manganese 2UniRule annotation
Metal bindingi262 – 2621Magnesium or manganese 2UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi129 – 18052ATPUniRule annotationAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. D-alanine-D-alanine ligase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  3. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationSAAS annotation

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradationUniRule annotationSAAS annotation, Peptidoglycan synthesisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotationSAAS annotation, MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciFNOV984129:GLDD-158-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:FNFX1_0157Imported
OrganismiFrancisella cf. novicida (strain Fx1)Imported
Taxonomic identifieri984129 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaThiotrichalesFrancisellaceaeFrancisella
ProteomesiUP000008302: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Structurei

3D structure databases

ProteinModelPortaliF4BCW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini103 – 293191ATP-graspUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation
Contains ATP-grasp domain.SAAS annotation

Phylogenomic databases

KOiK01921.
OMAiKLAFQYD.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR23132. PTHR23132. 1 hit.
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4BCW3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKNEKIVVLY GGDSPEREVS LKSGKAVLDS LISQGYDAVG VDASGKELVA
60 70 80 90 100
KLLELKPDKC FVALHGEDGE NGRVSALLEM LEIKHTSSSM KSSVITMDKM
110 120 130 140 150
ISKEILMHHR MPTPMAKFLT DKLVAEDEIS FPVAVKPSSG GSSIATFKVK
160 170 180 190 200
SIQELKHAYE EASKYGEVMI EQWVTGKEIT VAIVNDEVYS SVWIEPQNEF
210 220 230 240 250
YDYESKYSGK SIYHSPSGLC EQKELEVRQL AKKAYDLLGC SGHARVDFIY
260 270 280 290
DDRGNFYIME INSSPGMTEN SLSPKSAAAE GVDFDSFVKR IIEQAQ
Length:296
Mass (Da):32,786
Last modified:June 28, 2011 - v1
Checksum:i8733294194062AA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002557 Genomic DNA. Translation: AEB27105.1.
RefSeqiWP_011733579.1. NC_017450.1.
YP_005825065.1. NC_017450.1.

Genome annotation databases

EnsemblBacteriaiAEB27105; AEB27105; FNFX1_0157.
GeneIDi12434946.
KEGGifcf:FNFX1_0157.
PATRICi54253859. VBIFraCf186347_0155.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002557 Genomic DNA. Translation: AEB27105.1 .
RefSeqi WP_011733579.1. NC_017450.1.
YP_005825065.1. NC_017450.1.

3D structure databases

ProteinModelPortali F4BCW3.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AEB27105 ; AEB27105 ; FNFX1_0157 .
GeneIDi 12434946.
KEGGi fcf:FNFX1_0157.
PATRICi 54253859. VBIFraCf186347_0155.

Phylogenomic databases

KOi K01921.
OMAi KLAFQYD.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci FNOV984129:GLDD-158-MONOMER.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPi MF_00047. Dala_Dala_lig.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR23132. PTHR23132. 1 hit.
Pfami PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view ]
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Common ancestry and novel genetic traits of Francisella novicida-like isolates from North America and Australia as revealed by comparative genomic analyses."
    Siddaramappa S., Challacombe J.F., Petersen J.M., Pillai S., Hogg G., Kuske C.R.
    Appl. Environ. Microbiol. 77:5110-5122(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Fx1Imported.

Entry informationi

Entry nameiF4BCW3_FRACF
AccessioniPrimary (citable) accession number: F4BCW3
Entry historyi
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: November 26, 2014
This is version 24 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteomeImported

External Data

Dasty 3