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F4ADJ7 (F4ADJ7_LACJH) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047
EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
ORF Names:LJP_0114 EMBL AEB92453.1
OrganismLactobacillus johnsonii DPC 6026 EMBL AEB92453.1
Taxonomic identifier909954 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus ›

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047 SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain134 – 344211ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2971Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3111Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3111Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3131Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F4ADJ7 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: EE507A8F1B42F377

FASTA36140,391
        10         20         30         40         50         60 
MTDKIKVGLI FGGNSSEYEV SIMSAHNIYE EIDTNKFDVY PMWITNDGYL ADDADSRKVL 

        70         80         90        100        110        120 
DNPQMEVANP HKVANISNII ELKDRPEIDV FFPIVHGNLG EDGCLQGLFR VLDKPFVGDD 

       130        140        150        160        170        180 
VLAAAVTMDK EMTKILAQRA GVPVAKWIAV KRFEYNDPDN EKLDYEYVAS QLGSDLFVKP 

       190        200        210        220        230        240 
SNQGSSVGVS HVTNEKEYKV ALAEAFKYDD KVLVEETVHG TEVETAVLGN DKPIVAGVGQ 

       250        260        270        280        290        300 
IINAKDSFYT YENKYDDDST STLEIPAKLP EGIVETVRKN ALKVFQATEC SGLARIDSML 

       310        320        330        340        350        360 
RSEDNEVVLT EVNALPGFTN ISMYPKLFEE IGIPYTDLIT KLIDYAMERY DHKKTLLHKH 


D

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References

[1]"Host Specific Diversity in Lactobacillus johnsonii as Evidenced by a Major Chromosomal Inversion and Phage Resistance Mechanisms."
Guinane C.M., Kent R.M., Norberg S., Hill C., Fitzgerald G.F., Stanton C., Ross R.P.
PLoS ONE 6:e18740-e18740(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: DPC 6026 EMBL AEB92453.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002464 Genomic DNA. Translation: AEB92453.1.
RefSeqYP_005861403.1. NC_017477.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEB92453; AEB92453; LJP_0114.
GeneID12469776.
KEGGljh:LJP_0114.
PATRIC54201053. VBILacJoh171132_0133.

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. PreATP-grasp-like. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF4ADJ7_LACJH
AccessionPrimary (citable) accession number: F4ADJ7
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 3, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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