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F4A747 (F4A747_CLOBO) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AEB76956.1
ORF Names:CbC4_2291 EMBL AEB76956.1
OrganismClostridium botulinum BKT015925 [Complete proteome] EMBL AEB76956.1
Taxonomic identifier929506 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesClostridiaceaeClostridium

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain129 – 335207ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding162 – 21756ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2881Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3021Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3021Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3041Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F4A747 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: F08C2740EF4BE4A5

FASTA34338,509
        10         20         30         40         50         60 
MKKKVGILFG GQSTEHEVSR VSASSVLKNI DLSKYDVYPI GITKDGKWFE YTGSVDNIES 

        70         80         90        100        110        120 
GEWEKDEFYK NPNGQEILFN REVDVVFPVM HGLYGEDGTI QGLCKLLDIP CVGPGVMSSA 

       130        140        150        160        170        180 
VCMDKVYTKY VLENFGIKQA DYVVVTAHDY KTIKEDILTK IENKLGYDVF IKPSNSGSSV 

       190        200        210        220        230        240 
GISKAHNREE LEKGLSEALK FDRKVLVETA LNAREIEVAV LGNDEPEAAT PGEIVPANEF 

       250        260        270        280        290        300 
YDYEAKYSNA ESKLLLPANL SEKKLEKVKE LAIRIYKMLD CSGMSRVDFL VDKETEEVYL 

       310        320        330        340 
NEINTIPGFT KISMYPKMWQ AEGKSYGNLI SEIIELAIER DNK 

« Hide

References

[1]"Clostridium botulinum group III: a group with dual identity shaped by plasmids, phages and mobile elements."
Skarin H., Hafstrom T., Westerberg J., Segerman B.
BMC Genomics 12:185-185(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: BKT015925 EMBL AEB76956.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002410 Genomic DNA. Translation: AEB76956.1.
RefSeqYP_004396953.1. NC_015425.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEB76956; AEB76956; CbC4_2291.
GeneID10465248.
KEGGcbn:CbC4_2291.
PATRIC54459976. VBICloBot178872_2603.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycCBOT929506:GHKW-2356-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF4A747_CLOBO
AccessionPrimary (citable) accession number: F4A747
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 11, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)