Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endonuclease III

Gene

nth

Organism
Mahella australiensis (strain DSM 15567 / CIP 107919 / 50-1 BON)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Catalytic activityi

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster.UniRule annotation
  • [4Fe-4S] clusterNote: Binds 1 [4Fe-4S] cluster.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi188 – 1881Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi195 – 1951Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi198 – 1981Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi204 – 2041Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

EndonucleaseImported, GlycosidaseUniRule annotation, Hydrolase, LyaseUniRule annotationImported, Nuclease

Keywords - Biological processi

DNA damage, DNA repairUniRule annotation

Keywords - Ligandi

4Fe-4SUniRule annotation, DNA-bindingUniRule annotation, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciMAUS697281:GH33-1964-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease IIIUniRule annotation (EC:4.2.99.18UniRule annotation)
Alternative name(s):
DNA-(apurinic or apyrimidinic site) lyaseUniRule annotation
Gene namesi
Name:nthUniRule annotation
Ordered Locus Names:Mahau_1918Imported
OrganismiMahella australiensis (strain DSM 15567 / CIP 107919 / 50-1 BON)Imported
Taxonomic identifieri697281 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacterales Family IV. Incertae SedisMahella
ProteomesiUP000008457 Componenti: Chromosome

Interactioni

Protein-protein interaction databases

STRINGi697281.Mahau_1918.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini100 – 12829HhHUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the Nth/MutY family.UniRule annotation
Contains 1 HhH domain.UniRule annotation

Phylogenomic databases

KOiK10773.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFiPIRSF001435. Nth. 1 hit.
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F4A1P4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTRDDIEHI LDILADCYPQ AKTALVYSNA FELLIATILS AQSTDKQVNK
60 70 80 90 100
VTGKLFGKYK TPEDFAALEP QTLEEEIKSC GLYRTKALNI INMSKILVER
110 120 130 140 150
YGSQVPSDPD ELQKLPGVGR KTANVVVSNA FGRPAIAVDT HVFRVTHRLG
160 170 180 190 200
LAKSSTPLGT EKELMACIPR VLWSQAHHWF IYHGRNVCRA RQPKCDECRL
210
RQYCDFYNSE AGL
Length:213
Mass (Da):23,988
Last modified:June 28, 2011 - v1
Checksum:i0D3885B3ECF4E51E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002360 Genomic DNA. Translation: AEE97094.1.
RefSeqiWP_013781522.1. NC_015520.1.
YP_004463916.1. NC_015520.1.

Genome annotation databases

EnsemblBacteriaiAEE97094; AEE97094; Mahau_1918.
KEGGimas:Mahau_1918.
PATRICi54632539. VBIMahAus157719_1958.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002360 Genomic DNA. Translation: AEE97094.1.
RefSeqiWP_013781522.1. NC_015520.1.
YP_004463916.1. NC_015520.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi697281.Mahau_1918.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEE97094; AEE97094; Mahau_1918.
KEGGimas:Mahau_1918.
PATRICi54632539. VBIMahAus157719_1958.

Phylogenomic databases

KOiK10773.

Enzyme and pathway databases

BioCyciMAUS697281:GH33-1964-MONOMER.

Family and domain databases

Gene3Di1.10.1670.10. 1 hit.
1.10.340.30. 1 hit.
HAMAPiMF_00942. Nth.
InterProiIPR011257. DNA_glycosylase.
IPR004036. Endonuclease-III-like_CS2.
IPR003651. Endouclease3_FeS-loop_motif.
IPR003265. HhH-GPD_domain.
IPR000445. HhH_motif.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR023170. HTH_base_excis_C.
IPR005759. Nth.
[Graphical view]
PfamiPF00633. HHH. 1 hit.
PF00730. HhH-GPD. 1 hit.
[Graphical view]
PIRSFiPIRSF001435. Nth. 1 hit.
SMARTiSM00478. ENDO3c. 1 hit.
SM00525. FES. 1 hit.
SM00278. HhH1. 1 hit.
[Graphical view]
SUPFAMiSSF48150. SSF48150. 1 hit.
TIGRFAMsiTIGR01083. nth. 1 hit.
PROSITEiPS01155. ENDONUCLEASE_III_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 15567 / CIP 107919 / 50-1 BONImported.

Entry informationi

Entry nameiF4A1P4_MAHA5
AccessioniPrimary (citable) accession number: F4A1P4
Entry historyi
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: June 24, 2015
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.