F3ZZJ0 (F3ZZJ0_MAHA5) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420
EC=6.3.5.3 HAMAP-Rule MF_00420

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II HAMAP-Rule MF_00420

Gene names

Name:	purL HAMAP-Rule MF_00420
Ordered Locus Names:	Mahau_1634

Organism

Mahella australiensis (strain DSM 15567 / CIP 107919 / 50-1 BON) [Complete proteome] [HAMAP] EMBL AEE96816.1

Taxonomic identifier

697281 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family IV. Incertae Sedis › Mahella › Mahella australiensis

Protein attributes

Sequence length	735 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity. HAMAP-Rule MF_00420
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00420.
Sequence similarities	Belongs to the FGAMS family. HAMAP-Rule MF_00420

Ontologies

Keywords
Biological process	Purine biosynthesis HAMAP-Rule MF_00420
Cellular component	Cytoplasm HAMAP-Rule MF_00420
Ligand	ATP-binding HAMAP-Rule MF_00420 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00420 EMBL AEE96816.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Nucleotide binding	100 – 111	12	ATP By similarity HAMAP-Rule MF_00420

Sequences

	Sequence		Length	Mass (Da)
	F3ZZJ0 [UniParc]. Last modified June 28, 2011. Version 1. Checksum: 3AB300E66DD51C07	FASTA	735	79,322
	10 20 30 40 50 60 MPSKQRWDEV GLTENEYRMI IDILGREPND LELNLYGVMW SEHCGYKHSR AMLKMFPTSG 70 80 90 100 110 120 PRILQGPGEN AGIVDIGDGL AVCMKVESHN HPSALEPYQG AATGMGGIIR DIFTMGSRPV 130 140 150 160 170 180 ALLDSLKFGP LDNPRSRYLM DGVVSGIAGY GNCIGIPTVA GEVGFDPCYT GNPLVNAMCV 190 200 210 220 230 240 GLIRHDEIQR GAAAGVGNSV MIVGHTTGRD GMGGASFASV EITAESEERR SAVQVGDPFM 250 260 270 280 290 300 EKLLLEACLE LFKTGCVVGI QDMGAAGIIS STCETAARAG TGIELDVALV PKRETGMTPT 310 320 330 340 350 360 QVMISESQER MLVIVEKGRE QEVYDIFDKW GLHAVVIGHV TDDGMLRVLD NGIKVGEVPA 370 380 390 400 410 420 KSLAEAPVYH PDYAKPDYID ELAAFNPLTL TLPDDMNDVL LKLLASPDIC SKRWVYRQYD 430 440 450 460 470 480 YMVRTDTAVP PGSGDAAVVR IKGTSKAIAL TIDSNGRYSY LDPYKGGMIA VAEAARNIVC 490 500 510 520 530 540 TGALPIAITD CLNFGTPEKP DIYWQFRESI VGMAEACRAL DTPVISGNVS FYNETDRGAI 550 560 570 580 590 600 YPTPTVGMAG VMEDVKHITT QAFKKDGDAI VLLGETKEEL GASQFLNIIY GMQKGKVPDI 610 620 630 640 650 660 NLEKESSVQK AALKAIQEGI ISSAHDCSDG GLAIALAESC ISGRKGAYVN LKFDIRTDAL 670 680 690 700 710 720 LFGESQSRIL ISLKPEYLDK LFVICESFGV PCQVIGTVCG DRLNVDVNGN KVVQLPIEEM 730 KDKWEGTIPA YMNGR

References

[1]

"The complete genome of Mahella australiensis DSM 15567." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Teshima H., Brettin T., Detter J.C., Han C., Tapia R., Land M., Hauser L., Markowitz V. , Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Pukall R., Steenblock K., Schneider S., Klenk H.-P., Eisen J.A. Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 15567 / CIP 107919 / 50-1 BON.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002360 Genomic DNA. Translation: AEE96816.1.
RefSeq	YP_004463638.1. NC_015520.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEE96816; AEE96816; Mahau_1634.
GeneID	10608392.
KEGG	mas:Mahau_1634.
PATRIC	54631963. VBIMahAus157719_1674.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01952.
Enzyme and pathway databases
BioCyc	MAUS697281:GH33-1676-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

F3ZZJ0_MAHA5

Accession

Primary (citable) accession number: F3ZZJ0

Entry history

Integrated into UniProtKB/TrEMBL:	June 28, 2011
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 14 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)