Skip Header

Contribute Send feedback
Read comments (?) or add your own

F3ZZB7 (F3ZZB7_MAHA5) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 17. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase HAMAP-Rule MF_01228
EC=6.1.1.10 HAMAP-Rule MF_01228
Alternative name(s):
Methionyl-tRNA synthetase HAMAP-Rule MF_01228
Gene names
Name:metG HAMAP-Rule MF_01228
Ordered Locus Names:Mahau_0523
OrganismMahella australiensis (strain DSM 15567 / CIP 107919 / 50-1 BON) [Complete proteome] [HAMAP] EMBL AEE95727.1
Taxonomic identifier697281 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family IV. Incertae Sedis › Mahella ›

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP-Rule MF_01228

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP-Rule MF_01228 SAAS SAAS023457

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01228

Subunit structure

Monomer By similarity. HAMAP-Rule MF_01228

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_01228.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily. HAMAP-Rule MF_01228

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Motif14 – 2411"HIGH" region By similarity HAMAP-Rule MF_01228
Motif299 – 3035"KMSKS" region By similarity HAMAP-Rule MF_01228

Sites

Metal binding1291Zinc By similarity HAMAP-Rule MF_01228
Metal binding1321Zinc By similarity HAMAP-Rule MF_01228
Metal binding1461Zinc By similarity HAMAP-Rule MF_01228
Metal binding1491Zinc By similarity HAMAP-Rule MF_01228

Sequences

Sequence LengthMass (Da)Tools
F3ZZB7 [UniParc].

Last modified June 28, 2011. Version 1.
Checksum: 056091A0618D0E5A

FASTA51258,949
        10         20         30         40         50         60 
MSKDKTYYIT TPIYYPSDKL HIGHSYTTVA ADAMARFKRM TGYDVWFLTG TDEHGEKIER 

        70         80         90        100        110        120 
VARQHNVTPQ QYVDDIVSWI KNLWETMDIS YDQFIRTTDP EHMKIVQTIF NRLYEQEDIY 

       130        140        150        160        170        180 
KGEYEGWYCT PCEAFWTELQ LVNGKCPDCG RPVERVREEA YFFKLSKYQD RLIEYIETHP 

       190        200        210        220        230        240 
DFIQPASRRN EMLNNFLRPG LEDLCVSRTS FSWGIPVTFD PKHVIYVWID ALSNYITALG 

       250        260        270        280        290        300 
AFTDHDEAFR KYWPADVHLV GKEIVRFHTI IWPAILMALG LELPKQVFGH GWLVMDGGKM 

       310        320        330        340        350        360 
SKSKGNVIDP LKLVEQYGVD AVRYFLLREV PFGADGNFSN EALVNRINSD LANDLGNLVS 

       370        380        390        400        410        420 
RTVAMVEKYF GGVLPVPSTA QSMDDGLKSM ALSTPKKMEE LMDKLQFSNA LSEIWALVAR 

       430        440        450        460        470        480 
SNKYIDETMP WVLARDEAQK ERLGTVLYNL TEVIRMISVM IQPYMPNTSP MIWRQIGLAE 

       490        500        510 
GPTTSWQSLY DFGAIEPGTM VKKGPALFPR IQ

« Hide

References

[1]"The complete genome of Mahella australiensis DSM 15567."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Pagani I., Ivanova N., Teshima H., Brettin T., Detter J.C., Han C., Tapia R., Land M., Hauser L., Markowitz V. expand/collapse author list , Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S., Pukall R., Steenblock K., Schneider S., Klenk H.-P., Eisen J.A.
Submitted (NOV-2010) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 15567 / CIP 107919 / 50-1 BON.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002360 Genomic DNA. Translation: AEE95727.1.
RefSeqYP_004462549.1. NC_015520.1.

3D structure databases

ProteinModelPortalF3ZZB7.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEE95727; AEE95727; Mahau_0523.
GeneID10607253.
KEGGmas:Mahau_0523.
PATRIC54629639. VBIMahAus157719_0543.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01874.

Enzyme and pathway databases

BioCycMAUS697281:GH33-537-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
HAMAPMF_01228. Met_tRNA_synth_type2.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
[Graphical view]
PfamPF08264. Anticodon_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. metG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF3ZZB7_MAHA5
AccessionPrimary (citable) accession number: F3ZZB7
Entry history
Integrated into UniProtKB/TrEMBL: June 28, 2011
Last sequence update: June 28, 2011
Last modified: April 3, 2013
This is version 17 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info