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Protein

Alcohol oxidase 2

Gene

AOX2

Organism
Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Minor isoform of alcohol oxidase, which catalyzes the oxidation of methanol to formaldehyde and hydrogen peroxide, the first step in the methanol utilization pathway of methylotrophic yeasts.1 Publication

Catalytic activityi

A primary alcohol + O2 = an aldehyde + H2O2.

Cofactori

FADBy similarity

Pathwayi: methane degradation

This protein is involved in the pathway methane degradation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway methane degradation and in Energy metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei567 – 5671Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 3831FADBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Methanol utilization

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

BRENDAi1.1.3.13. 4827.
UniPathwayiUPA00147.

Protein family/group databases

CAZyiAA3. Auxiliary Activities 3.

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol oxidase 2 (EC:1.1.3.13)
Short name:
AO 2
Short name:
AOX 2
Alternative name(s):
Methanol oxidase 2
Short name:
MOX 2
Gene namesi
Name:AOX2
Ordered Locus Names:PP7435_Chr4-0863
OrganismiKomagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1) (Yeast)
Taxonomic identifieri981350 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesPhaffomycetaceaeKomagataella
Proteomesi
  • UP000006853 Componenti: Chromosome 4

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 663662Alcohol oxidase 2PRO_0000425716Add
BLAST

Expressioni

Inductioni

Induced by methanol. Subject to strong carbon catabolite repression.1 Publication

Interactioni

Subunit structurei

Homooctamer.By similarity

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi661 – 6633Microbody targeting signalSequence analysis

Domaini

The C-terminal peroxisomal targeting signal (PTS) is essential for the efficient targeting and import of AOX into peroxisomes via the PTS1 pathway.By similarity

Sequence similaritiesi

Belongs to the GMC oxidoreductase family.Curated

Phylogenomic databases

OrthoDBiEOG092C10SH.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F2R038-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIPEEFDIL VLGGGSSGSC IAGRLANLDH SLKVGLIEAG ENNLNNPWVY
60 70 80 90 100
LPGIYPRNMK LDSKTASFYT SNPSPHLNGR RAIVPCANIL GGGSSINFMM
110 120 130 140 150
YTRGSASDYD DFEAEGWKTK DLLPLMKKTE TYQRACNNPE IHGFEGPIKV
160 170 180 190 200
SFGNYTYPVC QDFLRATESQ GIPYVDDLED LVTAHGAEHW LKWINRDTGR
210 220 230 240 250
RSDSAHAFVH STMRNHDNLY LICNTKVDKI IVEDGRAAAV RTVPSKPLNA
260 270 280 290 300
KKPTHKVYRA RKQIVLSCGT ISSPLVLQRS GFGDPIKLRA AGVKPLVNLP
310 320 330 340 350
GVGRNFQDHY CFFSPYRIKP QYESFDDFVR GDANIQKKVF DQWYANGTGP
360 370 380 390 400
LATNGIEAGV KIRPTPEELS QMDESFQEGY REYFEDKPDK PVMHYSIIAG
410 420 430 440 450
FFGDHTKIPP GKYMTMFHFL EYPFSRGSIH ITSPDPYATP DFDPGFMNDE
460 470 480 490 500
RDMAPMVWSY KKSRETARKM DHFAGEVTSH HPLFPYSSEA RAYEMDLETS
510 520 530 540 550
NAYGGPLNLT AGLAHGSWTQ PLKKPAGRNE GHVTSNQVEL HPDIEYDEED
560 570 580 590 600
DKAIENYIRE HTETTWHCLG TCSIGPREGS KIVKWGGVLD HRSNVYGVKG
610 620 630 640 650
LKVGDLSVCP DNVGCNTYTT ALLIGEKTAT LVGEDLGYTG EALDMTVPQF
660
KLGTYEKTGL ARF
Length:663
Mass (Da):73,952
Last modified:May 31, 2011 - v1
Checksum:i9A9D2C84161991B2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti262 – 2621K → T in AAB57850 (PubMed:2660463).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96968 Genomic DNA. Translation: AAB57850.1.
FR839631 Genomic DNA. Translation: CCA41016.1.

Genome annotation databases

EnsemblFungiiCCA41016; CCA41016; PP7435_Chr4-0863.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U96968 Genomic DNA. Translation: AAB57850.1.
FR839631 Genomic DNA. Translation: CCA41016.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiAA3. Auxiliary Activities 3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCCA41016; CCA41016; PP7435_Chr4-0863.

Phylogenomic databases

OrthoDBiEOG092C10SH.

Enzyme and pathway databases

UniPathwayiUPA00147.
BRENDAi1.1.3.13. 4827.

Family and domain databases

Gene3Di3.50.50.60. 3 hits.
InterProiIPR023753. FAD/NAD-binding_dom.
IPR012132. GMC_OxRdtase.
IPR000172. GMC_OxRdtase_N.
IPR007867. GMC_OxRtase_C.
[Graphical view]
PfamiPF05199. GMC_oxred_C. 1 hit.
PF00732. GMC_oxred_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000137. Alcohol_oxidase. 1 hit.
SUPFAMiSSF51905. SSF51905. 2 hits.
PROSITEiPS00623. GMC_OXRED_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiALOX2_KOMPC
AccessioniPrimary (citable) accession number: F2R038
Secondary accession number(s): Q9URI7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 19, 2014
Last sequence update: May 31, 2011
Last modified: September 7, 2016
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.