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F2NKW2 (F2NKW2_MARHT) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 19. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:Marky_0398 EMBL AEB11151.1
OrganismMarinithermus hydrothermalis (strain DSM 14884 / JCM 11576 / T1) [Complete proteome] [HAMAP] EMBL AEB11151.1
Taxonomic identifier869210 [NCBI]
Taxonomic lineageBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeMarinithermus

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS011095

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS011095

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS011095

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS011095.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS011095

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain123 – 320198ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding149 – 20254ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2741Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2871Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding2871Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding2891Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F2NKW2 [UniParc].

Last modified May 31, 2011. Version 1.
Checksum: BD0C5C2440B90625

FASTA32435,396
        10         20         30         40         50         60 
MSKLRILLIA GGRSGEHEVS LASARGVLEA MPHPTDLAVI AKDGRWLTGT AARAALEAGR 

        70         80         90        100        110        120 
AQRGEHPFPP PLSWHRYDVA FPLLHGRWGE DGTIQGFLEM LGLPYVGAGV GAAALCMDKE 

       130        140        150        160        170        180 
LTKRTLAHAG IPVVPWVSLW RGEGLPELPF SPPYFVKPAN TGSSVGISKV RTREALGPAL 

       190        200        210        220        230        240 
EEAFRWDDRV LVEQGLEGVR ELEVALLGNV TAEASVVGEV RYQSEFYDYA TKYTPGRAEL 

       250        260        270        280        290        300 
LIPAPVDADV ARRLQEVART AYRLLGVRGL ARVDFFLAPD GALYLNELNT MPGFTPTSMY 

       310        320 
PKLWQASGLE YEALLERLVR LALE 

« Hide

References

[1]"Complete genome sequence of the aerobic, heterotroph Marinithermus hydrothermalis type strain (T1(T)) from a deep-sea hydrothermal vent chimney."
Copeland A., Gu W., Yasawong M., Lapidus A., Lucas S., Deshpande S., Pagani I., Tapia R., Cheng J.F., Goodwin L.A., Pitluck S., Liolios K., Ivanova N., Mavromatis K., Mikhailova N., Pati A., Chen A., Palaniappan K. expand/collapse author list , Land M., Pan C., Brambilla E.M., Rohde M., Tindall B.J., Sikorski J., Goker M., Detter J.C., Bristow J., Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P., Woyke T.
Stand. Genomic Sci. 6:21-30(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14884 / JCM 11576 / T1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002630 Genomic DNA. Translation: AEB11151.1.
RefSeqYP_004367261.1. NC_015387.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEB11151; AEB11151; Marky_0398.
GeneID10433283.
KEGGmhd:Marky_0398.
PATRIC54433137. VBIMarHyd166763_0400.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycMHYD869210:GHEE-413-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF2NKW2_MARHT
AccessionPrimary (citable) accession number: F2NKW2
Entry history
Integrated into UniProtKB/TrEMBL: May 31, 2011
Last sequence update: May 31, 2011
Last modified: June 11, 2014
This is version 19 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)