F2ND57 (F2ND57_DESAR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
RNA 3'-terminal phosphate cyclase HAMAP-Rule MF_00200
Short name=RNA cyclase HAMAP-Rule MF_00200
Short name=RNA-3'-phosphate cyclase HAMAP-Rule MF_00200
EC=6.5.1.4 HAMAP-Rule MF_00200

Gene names

Name:	rtcA HAMAP-Rule MF_00200
Ordered Locus Names:	Desac_1948

Organism

Desulfobacca acetoxidans (strain ATCC 700848 / DSM 11109 / ASRB2) [Complete proteome] [HAMAP] EMBL AEB09781.1

Taxonomic identifier

880072 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Syntrophobacterales › Syntrophaceae › Desulfobacca › Desulfobacca acetoxidans

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing By similarity. HAMAP-Rule MF_00200
Catalytic activity	ATP + RNA 3'-terminal-phosphate = AMP + diphosphate + RNA terminal-2',3'-cyclic-phosphate. HAMAP-Rule MF_00200
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00200.
Sequence similarities	Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily. HAMAP-Rule MF_00200

Ontologies

Keywords
Cellular component	Cytoplasm HAMAP-Rule MF_00200
Ligand	ATP-binding HAMAP-Rule MF_00200 Nucleotide-binding
Molecular function	Ligase HAMAP-Rule MF_00200 EMBL AEB09781.1
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	RNA processing Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-3'-phosphate cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	306	1	Tele-AMP-histidine intermediate By similarity HAMAP-Rule MF_00200
	Binding site	104	1	ATP By similarity HAMAP-Rule MF_00200

Sequences

	Sequence		Length	Mass (Da)
	F2ND57 [UniParc]. Last modified May 31, 2011. Version 1. Checksum: 7093A29C8E012AF5	FASTA	344	36,907
	10 20 30 40 50 60 MLVLDGSYGE GGGQIIRTSL SLAALTGNAI RLDRIRYGRR QPGLRPQHLT AVRALAQVTH 70 80 90 100 110 120 AALTGDEIGS TCLTFSPQGI FPGNYTFDVA EKTGSAGSVC LIAQTLLPVL IFADAPSSLI 130 140 150 160 170 180 IKGGTHVPWS PPAHYLNAVF LPALREMGLN ADLEIIRWGF YPLGGGEIRL RLAPVQELNA 190 200 210 220 230 240 IDWTDPPAAT DFQGLSAAAN LPAHVTRRQM QALQQSLDWP LRILEEKAPS RGKGSFVFLW 250 260 270 280 290 300 GPHAGFSALG ARGKPAESVA LEAAMALKRF LTLRTGVDCR LADQIALYAA LAAGVSRFSS 310 320 330 340 EMITSHLLTN IWVIDRFLGQ RFAVQGNLGG TGIIMVKGSR GFNR

References

[1]

"The complete genome of Desulfobacca acetoxidans DSM 11109." US DOE Joint Genome Institute (JGI-PGF) Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Peters L., Kyrpides N., Mavromatis K., Ivanova N., Ovchinnikova G., Teshima H., Detter J.C., Han C., Land M., Hauser L., Markowitz V., Cheng J.-F. , Hugenholtz P., Woyke T., Wu D., Spring S., Schueler E., Brambilla E., Klenk H.-P., Eisen J.A. Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700848 / DSM 11109 / ASRB2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP002629 Genomic DNA. Translation: AEB09781.1.
RefSeq	YP_004370962.1. NC_015388.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AEB09781; AEB09781; Desac_1948.
GeneID	10437163.
KEGG	dao:Desac_1948.
PATRIC	54441199. VBIDesAce170587_2093.
Organism-specific databases
CMR	Search...
Phylogenomic databases
KO	K01974.
Enzyme and pathway databases
BioCyc	DACE880072:GHK9-1981-MONOMER.
Family and domain databases
Gene3D	3.30.360.20. 1 hit. 3.65.10.20. 2 hits.
HAMAP	MF_00200. RTC.
InterPro	IPR013791. RNA3'-term_phos_cycl_insert. IPR023797. RNA3'_phos_cyclase_dom. IPR000228. RNA3'_term_phos_cyc. IPR017770. RNA3'_term_phos_cyc_type_1. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
PANTHER	PTHR11096. PTHR11096. 1 hit.
Pfam	PF01137. RTC. 1 hit. PF05189. RTC_insert. 1 hit. [Graphical view]
SUPFAM	SSF52913. RNA3'-term_phos_cycl_insert. 1 hit. SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 2 hits.
TIGRFAMs	TIGR03399. RNA_3prim_cycl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

F2ND57_DESAR

Accession

Primary (citable) accession number: F2ND57

Entry history

Integrated into UniProtKB/TrEMBL:	May 31, 2011
Last sequence update:	May 31, 2011
Last modified:	May 1, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)