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F2MBL1 (F2MBL1_LACCC) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 20. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL AEA52463.1
Ordered Locus Names:LC2W_0127 EMBL AEA52463.1
OrganismLactobacillus casei (strain LC2W) [Complete proteome] [HAMAP] EMBL AEA52463.1
Taxonomic identifier999378 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length357 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain145 – 339195ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding172 – 22554ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2941Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3061Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3081Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F2MBL1 [UniParc].

Last modified May 31, 2011. Version 1.
Checksum: EE1EF6A679CAA405

FASTA35739,020
        10         20         30         40         50         60 
MKIVVLAGGR STERNVSISS GYRITNALRQ KGQQATFIDL FLGYDLEGKT VEQVFDDANT 

        70         80         90        100        110        120 
SKDLNISDAI LTDEDINKLR PDGSTQLFGP NVMAICKAAD IVFLALHGGD GENGKVQAVF 

       130        140        150        160        170        180 
DINGVKYTGS GPLASGITMN KVFSKEVMLY HGIQTSGFKE FKRDQGPKQT VPFDFPVVVK 

       190        200        210        220        230        240 
PTSGGSSVGT HIIHNQEELE SGLEDVFRFD NSAIVEEFTP GREFSLGVVN GHAYSAIEIK 

       250        260        270        280        290        300 
VRSGWYDFKH KFQAGYTDFI TPPKDLDEDV HQAMKDVAVQ TMDVLGLQNY GRIDFFANEK 

       310        320        330        340        350 
GVWVIEANNL PGMTPLSLLP QEAEADGVDY GDLVMDIVNG KLKLYADGMT EAGLLTK 

« Hide

References

[1]"Complete genome sequence of the probiotic bacterium Lactobacillus casei LC2W."
Chen C., Ai L., Zhou F., Wang L., Zhang H., Chen W., Guo B.
J. Bacteriol. 193:3419-3420(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: LC2W EMBL AEA52463.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002616 Genomic DNA. Translation: AEA52463.1.
RefSeqYP_005855047.1. NC_017473.1.

3D structure databases

ProteinModelPortalF2MBL1.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEA52463; AEA52463; LC2W_0127.
GeneID12463218.
KEGGlce:LC2W_0127.
PATRIC54297456. VBILacCas186771_0135.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.

Enzyme and pathway databases

BioCycLCAS999378:GLF5-133-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF2MBL1_LACCC
AccessionPrimary (citable) accession number: F2MBL1
Entry history
Integrated into UniProtKB/TrEMBL: May 31, 2011
Last sequence update: May 31, 2011
Last modified: February 19, 2014
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)