ID   F2LXE4_HIPMA            Unreviewed;       397 AA.
AC   F2LXE4;
DT   31-MAY-2011, integrated into UniProtKB/TrEMBL.
DT   31-MAY-2011, sequence version 1.
DT   27-MAR-2024, entry version 51.
DE   RecName: Full=3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|RuleBase:RU365103};
DE            Short=Kdo transferase {ECO:0000256|RuleBase:RU365103};
DE            EC=2.4.99.12 {ECO:0000256|RuleBase:RU365103};
DE   AltName: Full=Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|RuleBase:RU365103};
GN   OrderedLocusNames=Hipma_1300 {ECO:0000313|EMBL:AEA34258.1};
OS   Hippea maritima (strain ATCC 700847 / DSM 10411 / MH2).
OC   Bacteria; Campylobacterota; Desulfurellia; Desulfurellales; Hippeaceae;
OC   Hippea.
OX   NCBI_TaxID=760142 {ECO:0000313|EMBL:AEA34258.1, ECO:0000313|Proteomes:UP000008139};
RN   [1] {ECO:0000313|EMBL:AEA34258.1, ECO:0000313|Proteomes:UP000008139}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700847 / DSM 10411 / MH2
RC   {ECO:0000313|Proteomes:UP000008139};
RX   PubMed=21886857;
RA   Huntemann M., Lu M., Nolan M., Lapidus A., Lucas S., Hammon N.,
RA   Deshpande S., Cheng J.F., Tapia R., Han C., Goodwin L., Pitluck S.,
RA   Liolios K., Pagani I., Ivanova N., Ovchinikova G., Pati A., Chen A.,
RA   Palaniappan K., Land M., Hauser L., Jeffries C.D., Detter J.C.,
RA   Brambilla E.M., Rohde M., Spring S., Goker M., Woyke T., Bristow J.,
RA   Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.,
RA   Mavromatis K.;
RT   "Complete genome sequence of the thermophilic sulfur-reducer Hippea
RT   maritima type strain (MH(2)).";
RL   Stand. Genomic Sci. 4:303-311(2011).
RN   [2] {ECO:0000313|Proteomes:UP000008139}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700847 / DSM 10411 / MH2
RC   {ECO:0000313|Proteomes:UP000008139};
RG   US DOE Joint Genome Institute (JGI-PGF);
RA   Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S.,
RA   Peters L., Kyrpides N., Mavromatis K., Pagani I., Ivanova N.,
RA   Mikhailova N., Lu M., Detter J.C., Tapia R., Han C., Land M., Hauser L.,
RA   Markowitz V., Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Spring S.,
RA   Schroeder M., Brambilla E., Klenk H.-P., Eisen J.A.;
RT   "The complete genome of Hippea maritima DSM 10411.";
RL   Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes
CC       the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-
CC       Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate
CC       precursor of lipid A. {ECO:0000256|RuleBase:RU365103}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) =
CC         alpha-Kdo-(2->6)-lipid IVA (E. coli) + CMP + H(+);
CC         Xref=Rhea:RHEA:28066, ChEBI:CHEBI:15378, ChEBI:CHEBI:58603,
CC         ChEBI:CHEBI:60364, ChEBI:CHEBI:60377, ChEBI:CHEBI:85987;
CC         EC=2.4.99.12; Evidence={ECO:0000256|RuleBase:RU365103};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC       {ECO:0000256|RuleBase:RU365103}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU365103}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC       {ECO:0000256|RuleBase:RU365103}.
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DR   EMBL; CP002606; AEA34258.1; -; Genomic_DNA.
DR   RefSeq; WP_013682290.1; NC_015318.1.
DR   AlphaFoldDB; F2LXE4; -.
DR   STRING; 760142.Hipma_1300; -.
DR   KEGG; hmr:Hipma_1300; -.
DR   eggNOG; COG1519; Bacteria.
DR   HOGENOM; CLU_036146_2_0_7; -.
DR   InParanoid; F2LXE4; -.
DR   UniPathway; UPA00958; -.
DR   Proteomes; UP000008139; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043842; F:Kdo transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.11720; 3-Deoxy-D-manno-octulosonic-acid transferase, N-terminal domain; 1.
DR   Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR   InterPro; IPR007507; Glycos_transf_N.
DR   InterPro; IPR038107; Glycos_transf_N_sf.
DR   InterPro; IPR039901; Kdotransferase.
DR   PANTHER; PTHR42755:SF1; 3-DEOXY-D-MANNO-OCTULOSONIC ACID TRANSFERASE, MITOCHONDRIAL-RELATED; 1.
DR   PANTHER; PTHR42755; 3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE; 1.
DR   Pfam; PF04413; Glycos_transf_N; 1.
PE   3: Inferred from homology;
KW   Cell membrane {ECO:0000256|RuleBase:RU365103};
KW   Lipopolysaccharide biosynthesis {ECO:0000256|RuleBase:RU365103};
KW   Membrane {ECO:0000256|RuleBase:RU365103};
KW   Reference proteome {ECO:0000313|Proteomes:UP000008139};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365103}.
FT   DOMAIN          34..195
FT                   /note="3-deoxy-D-manno-octulosonic-acid transferase N-
FT                   terminal"
FT                   /evidence="ECO:0000259|Pfam:PF04413"
FT   ACT_SITE        58
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639901-1"
FT   SITE            120
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639901-2"
FT   SITE            195
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR639901-2"
SQ   SEQUENCE   397 AA;  46394 MW;  A7C860C99757481A CRC64;
     MLILYNLLIL FLSIAAAPLI LIKSITDKRL RYRIKDRFLP KSINEKDYIL FHASSFGETK
     TLFSVKEFFE KELNSKVVFS VFTDTAHKLL NENGILSPID FYPLYAKIFK HPPKIALFFE
     TEIWPSYLSF LKKRNTKLVL INARMSNSTY KTYKRFGFFF KRIISVFDLI IAKSHEDAKR
     FKYFNTNTIV CGNLKQYKKP QKFNPDNLKK TFLIQSTKPV LTLASFHKEE IDIAIEIIDK
     LRNDFFIVLA PRHLEDVPLF EKQLQLNQIP FSNRTSKKQP STVLLLDTMG ELEGIYSFTD
     VCIVGGSFHE NLKGHNPIEP LFYNNVVICG PFMESFSEEV EQLKKLNMIN QLKDTKSKDL
     IESIKKSKKV DSSDYFKHLE YILNCYLINT KNLLKHN
//