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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Fluviicola taffensis (strain DSM 16823 / RW262 / RW262)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotationSAAS annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+SAAS annotation, Mn2+SAAS annotation
  • Mg2+UniRule annotation, Mn2+UniRule annotation
  • thiamine diphosphateSAAS annotationNote: Binds 1 thiamine pyrophosphate per subunit.SAAS annotation
  • thiamine diphosphateUniRule annotationNote: Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. manganese ion binding Source: UniProtKB-HAMAP
  4. thiamine pyrophosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

TransferaseUniRule annotationSAAS annotation

Keywords - Biological processi

Menaquinone biosynthesisUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotationSAAS annotation, ManganeseUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Thiamine pyrophosphateUniRule annotationSAAS annotation

Enzyme and pathway databases

BioCyciFTAF755732:GHMH-280-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotationSAAS annotation (EC:2.2.1.9UniRule annotationSAAS annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:Fluta_0276Imported
OrganismiFluviicola taffensis (strain DSM 16823 / RW262 / RW262)Imported
Taxonomic identifieri755732 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesCryomorphaceaeFluviicola
ProteomesiUP000007463: Chromosome

Interactioni

Subunit structurei

Homodimer.UniRule annotationSAAS annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

KOiK02551.
OMAiQKPWLLE.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

F2ICQ8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISSAKLEVQ LLVDGCKKVG VTHVVISPGS RNAPLSIAFD EDPDFQVFVV
60 70 80 90 100
PDERVAAFYA LGMAQKLNQP VVITCTSGSA PLNYYPAIAE AFYQEIPLIV
110 120 130 140 150
LSADRPAEWL DQGDGQTIRQ EGVFNQMVLN SAQLNEIHTD NQRWYFERKL
160 170 180 190 200
SEALQTAVGN RKGPIHLNIP FSEPLYETTS DIQAMSNWIE LAETECVLTK
210 220 230 240 250
NSRSKLESIW SNSEKRLIIV GQQQNDSHLQ ELLESLALDG SVAVLVEHTS
260 270 280 290 300
NLFSPYFVSN IDRALNIIPE SDLSSFYPDC IVTIGGAIIS KKIKVFLRQS
310 320 330 340 350
KAPVIRFGVD FPFMDTFQNL KFTASISAIS GIQLFHSFIK EKPHKSRFGF
360 370 380 390 400
AWKKIDQFGA EEQKQICNAQ PWSDLKAMEF VMDVIPDQCN LHISNSSLIR
410 420 430 440 450
YALLFDPIKT LKYWCNRGTS GIDGSSSTAV GSAIVSSKES HVLVTGDLSF
460 470 480 490 500
FYDSNAWWSK YLPSNLRIIL VNNGGGDIFN IIPGPKSTNQ WKKIFVAEQE
510 520 530 540 550
LSAENIAKTY GLDYFEASSL SDLEGNLELF FGESSNDKPK ILEINTMNEQ
560
NSAVLETYFK SFKKRV
Length:566
Mass (Da):63,290
Last modified:May 31, 2011 - v1
Checksum:iDBC47A1D603F31D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002542 Genomic DNA. Translation: AEA42285.1.
RefSeqiWP_013685059.1. NC_015321.1.
YP_004343123.1. NC_015321.1.

Genome annotation databases

EnsemblBacteriaiAEA42285; AEA42285; Fluta_0276.
GeneIDi10395605.
KEGGifte:Fluta_0276.
PATRICi54414798. VBIFluTaf147081_0274.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP002542 Genomic DNA. Translation: AEA42285.1.
RefSeqiWP_013685059.1. NC_015321.1.
YP_004343123.1. NC_015321.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAEA42285; AEA42285; Fluta_0276.
GeneIDi10395605.
KEGGifte:Fluta_0276.
PATRICi54414798. VBIFluTaf147081_0274.

Phylogenomic databases

KOiK02551.
OMAiQKPWLLE.

Enzyme and pathway databases

UniPathwayiUPA00079.
UPA01057; UER00164.
BioCyciFTAF755732:GHMH-280-MONOMER.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 16823 / RW262 / RW262Imported.

Entry informationi

Entry nameiF2ICQ8_FLUTR
AccessioniPrimary (citable) accession number: F2ICQ8
Entry historyi
Integrated into UniProtKB/TrEMBL: May 31, 2011
Last sequence update: May 31, 2011
Last modified: February 4, 2015
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.