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F2ICG2 (F2ICG2_FLUTR) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 16. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Riboflavin biosynthesis protein RibBA HAMAP-Rule MF_01283
Gene names
Name:ribBA HAMAP-Rule MF_01283
Ordered Locus Names:Fluta_3460
OrganismFluviicola taffensis (strain DSM 16823 / RW262 / RW262) [Complete proteome] [HAMAP] EMBL AEA45432.1
Taxonomic identifier755732 [NCBI]
Taxonomic lineageBacteria › Bacteroidetes › Flavobacteriia › Flavobacteriales › Cryomorphaceae › Fluviicola ›

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Catalyzes the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (DARP), formate and pyrophosphate By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Catalytic activity

D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP-Rule MF_01283 SAAS SAAS017945

GTP + 3 H2O = formate + 2,5-diamino-6-hydroxy-4-(5-phospho-D-ribosylamino)pyrimidine + diphosphate. HAMAP-Rule MF_01283 SAAS SAAS017945

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. HAMAP-Rule MF_01283 SAAS SAAS017945

Pathway

Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP-Rule MF_01283 SAAS SAAS017945

Cofactor biosynthesis; riboflavin biosynthesis; 5-amino-6-(D-ribitylamino)uracil from GTP: step 1/4. HAMAP-Rule MF_01283 SAAS SAAS017945

Sequence similarities

In the C-terminal section; belongs to the GTP cyclohydrolase II family. HAMAP-Rule MF_01283

In the N-terminal section; belongs to the DHBP synthase family. HAMAP-Rule MF_01283

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding255 – 2595GTP By similarity HAMAP-Rule MF_01283
Nucleotide binding298 – 3003GTP By similarity HAMAP-Rule MF_01283
Region1 – 204204DHBP synthase By similarity HAMAP-Rule MF_01283
Region29 – 302D-ribulose 5-phosphate binding By similarity HAMAP-Rule MF_01283
Region143 – 1475D-ribulose 5-phosphate binding By similarity HAMAP-Rule MF_01283
Region205 – 404200GTP cyclohydrolase II By similarity HAMAP-Rule MF_01283

Sites

Active site3321Proton acceptor; for GTP cyclohydrolase activity By similarity HAMAP-Rule MF_01283
Active site3341Nucleophile; for GTP cyclohydrolase activity By similarity HAMAP-Rule MF_01283
Metal binding301Magnesium or manganese 1 By similarity HAMAP-Rule MF_01283
Metal binding301Magnesium or manganese 2 By similarity HAMAP-Rule MF_01283
Metal binding1461Magnesium or manganese 2 By similarity HAMAP-Rule MF_01283
Metal binding2601Zinc; catalytic By similarity HAMAP-Rule MF_01283
Metal binding2711Zinc; catalytic By similarity HAMAP-Rule MF_01283
Metal binding2731Zinc; catalytic By similarity HAMAP-Rule MF_01283
Binding site341D-ribulose 5-phosphate By similarity HAMAP-Rule MF_01283
Binding site1671D-ribulose 5-phosphate By similarity HAMAP-Rule MF_01283
Binding site2761GTP By similarity HAMAP-Rule MF_01283
Binding site3201GTP By similarity HAMAP-Rule MF_01283
Binding site3551GTP By similarity HAMAP-Rule MF_01283
Binding site3601GTP By similarity HAMAP-Rule MF_01283
Site1291Essential for DHBP synthase activity By similarity HAMAP-Rule MF_01283
Site1671Essential for DHBP synthase activity By similarity HAMAP-Rule MF_01283

Sequences

Sequence LengthMass (Da)Tools
F2ICG2 [UniParc].

Last modified May 31, 2011. Version 1.
Checksum: A1418CDC2E52C04C

FASTA40444,729
        10         20         30         40         50         60 
MSGKLNTIEE AIEDIKAGKV IIVVDDEDRE NEGDFIAAAE MVTPEMINFM ATHGRGLICT 

        70         80         90        100        110        120 
PLTESRCNEL ELNLMVTNNT VLHETQFTVS VDLIGHGCTT GISVFDRAKT IKALVSEDTK 

       130        140        150        160        170        180 
PEDLGRPGHI FPLRAKKGGV LQRTGHTEAA VDLARMAGFK PAGILVEILN EDGSMSRLPE 

       190        200        210        220        230        240 
LFEIAKKFDL KLISIADLIA YRMKHESLIT REVEVEMPTE HGDFKLIAFS VNSNGQEHLA 

       250        260        270        280        290        300 
LVKGTWEPKE PVLVRMHSSC LTGDIFGSCR CDCGPQLQHS MKMIQEEGKG VIVYLNQEGR 

       310        320        330        340        350        360 
GIGLLNKLKA YKLQEEGFDT VEANLKLGFQ ADEREYGIGA QILHELGVSK MRLMSNNPKK 

       370        380        390        400 
RTGLVGYGLE IIENVAIEVG RNKHNEKYLQ TKVDKMGHSL KMGK

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References

[1]"The complete genome of Fluviicola taffensis DSM 16823."
US DOE Joint Genome Institute (JGI-PGF)
Lucas S., Copeland A., Lapidus A., Bruce D., Goodwin L., Pitluck S., Kyrpides N., Mavromatis K., Ivanova N., Mikhailova N., Pagani I., Chertkov O., Detter J.C., Han C., Tapia R., Land M., Hauser L., Markowitz V. expand/collapse author list , Cheng J.-F., Hugenholtz P., Woyke T., Wu D., Tindall B., Pomrenke H.G., Brambilla E., Klenk H.-P., Eisen J.A.
Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 16823 / RW262 / RW262.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002542 Genomic DNA. Translation: AEA45432.1.
RefSeqYP_004346270.1. NC_015321.1.

3D structure databases

ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAEA45432; AEA45432; Fluta_3460.
GeneID10398830.
KEGGfte:Fluta_3460.
PATRIC54421348. VBIFluTaf147081_3506.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK14652.
OMADVHMALT.

Enzyme and pathway databases

BioCycFTAF755732:GHMH-3505-MONOMER.
UniPathwayUPA00275; UER00399.
UPA00275; UER00400.

Family and domain databases

Gene3D3.90.870.10. 1 hit.
HAMAPMF_00179. RibA.
MF_00180. RibB.
MF_01283. RibBA.
InterProIPR017945. DHBP_synth_RibB-like_a/b_dom.
IPR000422. DHBP_synthase_RibB.
IPR000926. GTP_CycHdrlaseII_RibA.
IPR016299. Riboflavin_synth_RibBA.
[Graphical view]
PfamPF00926. DHBP_synthase. 1 hit.
PF00925. GTP_cyclohydro2. 1 hit.
[Graphical view]
PIRSFPIRSF001259. RibA. 1 hit.
SUPFAMSSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMsTIGR00505. ribA. 1 hit.
TIGR00506. ribB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameF2ICG2_FLUTR
AccessionPrimary (citable) accession number: F2ICG2
Entry history
Integrated into UniProtKB/TrEMBL: May 31, 2011
Last sequence update: May 31, 2011
Last modified: May 1, 2013
This is version 16 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info