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F2HQ08 (F2HQ08_LACLV) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 23. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047 EMBL ADZ62991.1
Ordered Locus Names:CVCAS_0326 EMBL ADZ62991.1
OrganismLactococcus lactis subsp. lactis (strain CV56) [Complete proteome] [HAMAP] EMBL ADZ62991.1
Taxonomic identifier929102 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain132 – 335204ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding162 – 21756ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding2891Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3021Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3021Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3041Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
F2HQ08 [UniParc].

Last modified May 31, 2011. Version 1.
Checksum: 8E86996E1D2A8197

FASTA34938,693
        10         20         30         40         50         60 
MSKETLILLY GGRSAEREVS VLSAESVMRA VNYSRFVVKT YFITKGGEFI KTQEFTDKPA 

        70         80         90        100        110        120 
EEEKLLTNDL AESYPKISPA AIYEKAAVVF PVLHGPMGED GSIQGFLEIL RLAYVGPNIL 

       130        140        150        160        170        180 
SASSTMDKLL AKHVFEAVGV PQVPYVAAFA DENQGEIAQE VVEKLEFPVF VKPANMGSSV 

       190        200        210        220        230        240 
GISKVDDLAD LQPALSEAYK YDNRVVIEQG VDAREIECAV LGNNSDVSAT LPGEVVKDVE 

       250        260        270        280        290        300 
FYDYNSKYID NKIQMDIPAK VPADLAQKMQ EYAIKAYKAV NGTGLSRCDF FVTKDGNVYL 

       310        320        330        340 
NEINAIPGFT QWSMYPLLWE NMGLSYSDLI EKLVDLAKET FETRENHLL 

« Hide

References

[1]"Complete genome sequence of Lactococcus lactis subsp. lactis CV56, a probiotic strain isolated from the vaginas of healthy women."
Gao Y., Lu Y., Teng K.L., Chen M.L., Zheng H.J., Zhu Y.Q., Zhong J.
J. Bacteriol. 193:2886-2887(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CV56 EMBL ADZ62991.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP002365 Genomic DNA. Translation: ADZ62991.1.
RefSeqYP_005867725.1. NC_017486.1.

3D structure databases

ProteinModelPortalF2HQ08.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEF2HQ08.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADZ62991; ADZ62991; CVCAS_0326.
GeneID12476405.
KEGGllt:CVCAS_0326.
PATRIC47137935. VBILacLac178659_0339.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK01921.
OMAMDKIAMK.

Enzyme and pathway databases

BioCycLLAC929102:GLFG-342-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF2HQ08_LACLV
AccessionPrimary (citable) accession number: F2HQ08
Entry history
Integrated into UniProtKB/TrEMBL: May 31, 2011
Last sequence update: May 31, 2011
Last modified: April 16, 2014
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)