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F2HJ03 (F2HJ03_LACLV) Unreviewed, UniProtKB/TrEMBL

Last modified April 3, 2013. Version 18. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine hydroxymethyltransferase HAMAP-Rule MF_00051
Short name=SHMT HAMAP-Rule MF_00051
Short name=Serine methylase HAMAP-Rule MF_00051
EC=2.1.2.1 HAMAP-Rule MF_00051
Gene names
Name:glyA HAMAP-Rule MF_00051 EMBL ADZ63173.1
Ordered Locus Names:CVCAS_0511 EMBL ADZ63173.1
OrganismLactococcus lactis subsp. lactis (strain CV56) [Complete proteome] [HAMAP] EMBL ADZ63173.1
Taxonomic identifier929102 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus ›

Protein attributes

Sequence length415 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible interconversion of serine and glycine with tetrahydrofolate (THF) serving as the one-carbon carrier. This reaction serves as the major source of one-carbon groups required for the biosynthesis of purines, thymidylate, methionine, and other important biomolecules. Also exhibits THF-independent aldolase activity toward beta-hydroxyamino acids, producing glycine and aldehydes, via a retro-aldol mechanism By similarity. HAMAP-Rule MF_00051

Interconversion of serine and glycine By similarity. RuleBase RU000585

Catalytic activity

5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. RuleBase RU000585 HAMAP-Rule MF_00051

Cofactor

Pyridoxal phosphate By similarity. RuleBase RU000585 HAMAP-Rule MF_00051

Pathway

Amino-acid biosynthesis; glycine biosynthesis; glycine from L-serine: step 1/1. HAMAP-Rule MF_00051

One-carbon metabolism; tetrahydrofolate interconversion. RuleBase RU000585 HAMAP-Rule MF_00051

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00051

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00051.

Sequence similarities

Belongs to the SHMT family. RuleBase RU004104 HAMAP-Rule MF_00051

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Region125 – 1273Substrate binding By similarity HAMAP-Rule MF_00051
Region355 – 3573Substrate binding By similarity HAMAP-Rule MF_00051

Sites

Binding site351Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site551Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site571Substrate By similarity HAMAP-Rule MF_00051
Binding site641Substrate By similarity HAMAP-Rule MF_00051
Binding site651Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site991Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site1211Substrate; via carbonyl oxygen By similarity HAMAP-Rule MF_00051
Binding site1761Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site2041Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site2291Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site2361Pyridoxal phosphate By similarity HAMAP-Rule MF_00051
Binding site2621Pyridoxal phosphate; via amide nitrogen and carbonyl oxygen By similarity HAMAP-Rule MF_00051
Binding site3631Pyridoxal phosphate By similarity HAMAP-Rule MF_00051

Amino acid modifications

Modified residue2301N6-(pyridoxal phosphate)lysine By similarity HAMAP-Rule MF_00051

Sequences

Sequence LengthMass (Da)Tools
F2HJ03 [UniParc].

Last modified May 31, 2011. Version 1.
Checksum: 593559F38C4718C0

FASTA41544,790
        10         20         30         40         50         60 
MIFDKEDFES FDPELWAAIH AEEIRQQQNI ELIASENIVS KAVMAAQGSV LTNKYAEGYP 

        70         80         90        100        110        120 
GKRYYGGTEA VDVVENLAIE RAKELFGAKF ANVQPHSGSQ ANAAAYMALI QPGDTVLGMD 

       130        140        150        160        170        180 
LNAGGHLTHG ASVNFSGKTY HFVPYGVNSE TELLDYDEIL KIAKQVQPKL IVAGASAYSR 

       190        200        210        220        230        240 
LIDFAKFREI ADSVGAKLMV DMAHIAGLVA TGAHPNPLPY ADVVTTTTHK TLRGPRGGMI 

       250        260        270        280        290        300 
LTNDEALAKK INSAIFPGTQ GGPLEHVIAA KAVAFKEALD PEFTTYIEQV IKNTQAMAEE 

       310        320        330        340        350        360 
FAKVEGLRLI AGGSDNHLLN LKVLDLGING KEAQDLLDSV HITLNKEAIP DETLSPFKTS 

       370        380        390        400        410 
GVRIGAAAIT SRGFKEVEAK KVAQLVSEAL VNHDNQEKLA EVRKAALELT RQFPL

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References

[1]"Complete genome sequence of Lactococcus lactis subsp. lactis CV56, a probiotic strain isolated from the vaginas of healthy women."
Gao Y., Lu Y., Teng K.L., Chen M.L., Zheng H.J., Zhu Y.Q., Zhong J.
J. Bacteriol. 193:2886-2887(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CV56 EMBL ADZ63173.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP002365 Genomic DNA. Translation: ADZ63173.1.
RefSeqYP_005867907.1. NC_017486.1.

3D structure databases

ProteinModelPortalF2HJ03.
SMRF2HJ03. Positions 12-407.
ModBaseSearch...

Proteomic databases

PRIDEF2HJ03.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADZ63173; ADZ63173; CVCAS_0511.
GeneID12476610.
KEGGllt:CVCAS_0511.
PATRIC47138359. VBILacLac178659_0527.

Organism-specific databases

CMRSearch...

Phylogenomic databases

KOK00600.
OMALELTHQF.

Enzyme and pathway databases

UniPathwayUPA00193.
UPA00288; UER01023.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
HAMAPMF_00051. SHMT.
InterProIPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR001085. Ser_HO-MeTrfase.
IPR019798. Ser_HO-MeTrfase_PLP_BS.
[Graphical view]
PANTHERPTHR11680. PTHR11680. 1 hit.
PfamPF00464. SHMT. 1 hit.
[Graphical view]
PIRSFPIRSF000412. SHMT. 1 hit.
SUPFAMSSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
PROSITEPS00096. SHMT. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameF2HJ03_LACLV
AccessionPrimary (citable) accession number: F2HJ03
Entry history
Integrated into UniProtKB/TrEMBL: May 31, 2011
Last sequence update: May 31, 2011
Last modified: April 3, 2013
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info