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Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Pantoea ananatis (strain AJ13355)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotationSAAS annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotationSAAS annotation

Cofactori

Mg2+UniRule annotationSAAS annotation

Enzyme regulationi

Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
Active sitei128 – 1281Proton acceptorUniRule annotation
Binding sitei155 – 1551Allosteric activator ADPUniRule annotation
Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
Binding sitei212 – 2121Allosteric activator ADPUniRule annotation
Binding sitei223 – 2231SubstrateUniRule annotation
Binding sitei244 – 2441Substrate; shared with dimeric partnerUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi73 – 742ATPUniRule annotation
Nucleotide bindingi103 – 1064ATPUniRule annotation

GO - Molecular functioni

  1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fructose 6-phosphate metabolic process Source: InterPro
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotation, Transferase

Keywords - Biological processi

GlycolysisUniRule annotationSAAS annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotationSAAS annotation, Metal-bindingUniRule annotationSAAS annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciPANA932677:GLI8-3136-MONOMER.
UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:pfkAUniRule annotationImported
Ordered Locus Names:PAJ_3087Imported
OrganismiPantoea ananatis (strain AJ13355)Imported
Taxonomic identifieri932677 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePantoea
ProteomesiUP000006690: Chromosome

Subcellular locationi

Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotationSAAS annotation

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni55 – 606Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
Regioni126 – 1283Substrate bindingUniRule annotation
Regioni170 – 1723Substrate bindingUniRule annotation
Regioni186 – 1883Allosteric activator ADP bindingUniRule annotation
Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
Regioni250 – 2534Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

Phylogenomic databases

KOiK00850.
OMAiVHHDIVD.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F2EXL1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKIGVLTS GGDAPGMNAA IRGVVRSALS EGLEVFGIYD GYQGLYEDRM
60 70 80 90 100
INLDRYSVSD MINRGGTFLG SARFPEFRND DVRQVAIENL KKRGIDALVV
110 120 130 140 150
IGGDGSYMGA KRLTEMGFPC IGLPGTIDND VAGTDYTIGY FTALETVVEA
160 170 180 190 200
IDRLRDTSSS HQRISIVEVM GRYCGDLTLA AAIAGGCEFI VLPEIPYTRE
210 220 230 240 250
ELVAEIKAGI AKGKKHAIVA ITEHICDIDD LARFIETETK RETRATVLGH
260 270 280 290 300
IQRGGAPCAY DRILASRMGA YSIELLMQGY GGRCVGIQND KMVHHDIIDA
310 320
IENMKRPFKR DWMDTAKQLY
Length:320
Mass (Da):35,128
Last modified:May 31, 2011 - v1
Checksum:iD6E53F6A5749963F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP012032 Genomic DNA. Translation: BAK13167.1.
RefSeqiYP_005935963.1. NC_017531.1.

Genome annotation databases

EnsemblBacteriaiBAK13167; BAK13167; PAJ_3087.
GeneIDi12526415.
KEGGipaj:PAJ_3087.
PATRICi54227415. VBIPanAna188453_3580.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP012032 Genomic DNA. Translation: BAK13167.1.
RefSeqiYP_005935963.1. NC_017531.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAK13167; BAK13167; PAJ_3087.
GeneIDi12526415.
KEGGipaj:PAJ_3087.
PATRICi54227415. VBIPanAna188453_3580.

Phylogenomic databases

KOiK00850.
OMAiVHHDIVD.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.
UPA00109; UER00182.
BioCyciPANA932677:GLI8-3136-MONOMER.

Family and domain databases

HAMAPiMF_00339. Phosphofructokinase.
InterProiIPR012003. ATP_PFK_prok-type.
IPR012828. PFKA_ATP.
IPR022953. Phosphofructokinase.
IPR015912. Phosphofructokinase_CS.
IPR000023. Phosphofructokinase_dom.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Pantoea ananatis AJ13355."
    Hara Y.
    Submitted (DEC-2010) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AJ13355Imported.

Entry informationi

Entry nameiF2EXL1_PANAA
AccessioniPrimary (citable) accession number: F2EXL1
Entry historyi
Integrated into UniProtKB/TrEMBL: May 31, 2011
Last sequence update: May 31, 2011
Last modified: March 4, 2015
This is version 23 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.