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Protein

Ubiquitin carboxyl-terminal hydrolase 37

Gene

USP37

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-631 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei352 – 3521NucleophilePROSITE-ProRule annotation
Active sitei909 – 9091Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. ubiquitin thiolesterase activity Source: GO_Central

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. G1/S transition of mitotic cell cycle Source: UniProtKB
  3. mitotic nuclear division Source: UniProtKB-KW
  4. proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
  5. protein deubiquitination Source: GO_Central
  6. protein K11-linked deubiquitination Source: UniProtKB
  7. protein K48-linked deubiquitination Source: UniProtKB
  8. regulation of proteasomal protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 37 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 37
Ubiquitin thioesterase 37
Ubiquitin-specific-processing protease 37
Gene namesi
Name:USP37
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 15

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 982982Ubiquitin carboxyl-terminal hydrolase 37PRO_0000412646Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei631 – 6311Phosphoserine; by CDK2By similarity
Modified residuei653 – 6531PhosphoserineBy similarity
Modified residuei655 – 6551PhosphoserineBy similarity

Post-translational modificationi

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the APC(CDH1) complex during late mitosis, leading to its degradation. Able to mediate auto-deubiquitination (By similarity).By similarity
Phosphorylated at Ser-631 by CDK2 during G1/S phase but not during mitosis; phosphorylation at Ser-631 is required for deubiquitinase activity. Also polyubiquitinated during early G1 phase, without leading to degradation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Interactioni

Subunit structurei

Interacts with FZR1/CDH1.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini343 – 954612USPAdd
BLAST
Domaini707 – 72620UIM 1PROSITE-ProRule annotationAdd
BLAST
Domaini809 – 82820UIM 2PROSITE-ProRule annotationAdd
BLAST
Domaini831 – 85020UIM 3PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi32 – 343KEN box 1By similarity
Motifi71 – 799D-box 1By similarity
Motifi96 – 10510D-box 2By similarity
Motifi161 – 1699D-box 3By similarity
Motifi224 – 2263KEN box 2By similarity
Motifi785 – 7873KEN box 3By similarity

Domaini

The KEN box 3 is required for interaction with FZR1/CDH1 and is essential for APC(CDH1)-mediated ubiquitination.By similarity

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 3 UIM (ubiquitin-interacting motif) domains.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00440000033542.
InParanoidiF1SRY5.
OMAiLQEFNNS.
OrthoDBiEOG7HMS09.
TreeFamiTF323032.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR003903. Ubiquitin-int_motif.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02809. UIM. 3 hits.
[Graphical view]
SMARTiSM00726. UIM. 3 hits.
[Graphical view]
PROSITEiPS50330. UIM. 3 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F1SRY5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSPLKIQGPI RIRSMQTGIT KWKEGSFEIV EKENKVSLVV HYNTGGIPRI
60 70 80 90 100
FQLSHNIKNV VLRPSGAKQS RLMLTLQDNS FLSIDKVPSK DAEEMRLFLD
110 120 130 140 150
AVHQNRLNAV AMKPSQGSGS FGAILGSRTS QKETHRQLSY SDNQVSSKRG
160 170 180 190 200
SLETKDDIPF RKVLGNPGRA SIKTAAGSGI TATRTIPSLT STSTPLRSGL
210 220 230 240 250
LENRTEKRKR MLSSGSELNE DYPKENDSSS NNKAMTDPSR KYLTSSREKQ
260 270 280 290 300
LSLKQSEENR TSGLLPLQSS SFYGSRAASK DYSPSSTNLD RTNISSQTPS
310 320 330 340 350
AKRSLGFLPQ PAPLSVKKLR CNQDYTGWNK PRLPLSSHQQ QLQGFSNLGN
360 370 380 390 400
TCYMNAILQS LFSLQSFAND LLKQGIPWKK IPLNALISRR FAHLLVKKDI
410 420 430 440 450
CNSETKKDLL KKVKNAISAT AERFSGYMQN DAHEFLSQCL DQLKEDMEKL
460 470 480 490 500
NKTWKTEPVP GEENSPDVTA TRVYTCPVIT NLEFEVQHSI ICKACGEIIP
510 520 530 540 550
KREQFNDLSI DLPRRKKPLP PRSIQDSLDL FFRAEELEYS CEKCGGKCAL
560 570 580 590 600
VRHKFNRLPR ILILHLKRYS FNVALSLNNK IGQQVIIPRY LTLSSHCTEN
610 620 630 640 650
TKPPFNLGWS AQMAISRPLK ASQMVNSCIT SPSTPSKNFT FKSKSSLALS
660 670 680 690 700
LDSDSEDELK RSVALSQRLC EISSSEQQQE DLEKDSKSCK LEPDKSELEN
710 720 730 740 750
SGFDAMSEEE LLAAVLEISK REASPSPSHE DDDKPTSSPD TGFAEDDIQE
760 770 780 790 800
MPENPDPMET EKPKTITEPD PASFTEITKD CDENKENKTP EGSQGEVDWL
810 820 830 840 850
QQYDMERERE EQELQQALAQ SLQEQEAWEQ KEDDDLKRAT ELSLQEFNNS
860 870 880 890 900
FLDSLGSDED SGNEDVLDME YTEAEAEELK RNAETGNLPH SYRLISVVSH
910 920 930 940 950
IGSTSSSGHY ISDVYDIKKQ AWFTYNDLEV SKIQEASVQS DRDRSGYIFF
960 970 980
YMHKEIFDEL LETEKNSQAL SMEVGKTTRQ AS
Length:982
Mass (Da):110,483
Last modified:May 3, 2011 - v1
Checksum:i3B09ABDE42E07AFF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU570813 Genomic DNA. No translation available.

Genome annotation databases

EnsembliENSSSCT00000017635; ENSSSCP00000017157; ENSSSCG00000016194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU570813 Genomic DNA. No translation available.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000017635; ENSSSCP00000017157; ENSSSCG00000016194.

Phylogenomic databases

GeneTreeiENSGT00440000033542.
InParanoidiF1SRY5.
OMAiLQEFNNS.
OrthoDBiEOG7HMS09.
TreeFamiTF323032.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR003903. Ubiquitin-int_motif.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
PF02809. UIM. 3 hits.
[Graphical view]
SMARTiSM00726. UIM. 3 hits.
[Graphical view]
PROSITEiPS50330. UIM. 3 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Swine Genome Sequencing Consortium.
    Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Landrace.

Entry informationi

Entry nameiUBP37_PIG
AccessioniPrimary (citable) accession number: F1SRY5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: May 3, 2011
Last modified: February 4, 2015
This is version 20 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.