F1SRY5 (UBP37_PIG) Reviewed, UniProtKB/Swiss-Prot
Last modified
July 11, 2012.
Version 9.
History...
Clusters with 
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin carboxyl-terminal hydrolase 37 EC=3.4.19.12 Alternative name(s): Deubiquitinating enzyme 37 Ubiquitin thioesterase 37 Ubiquitin-specific-processing protease 37 | ||
| Gene names |
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| Organism | Sus scrofa (Pig) [Reference proteome] | ||
| Taxonomic identifier | 9823 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus |
Protein attributes
| Sequence length | 982 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology |
General annotation (Comments)
| Function | Deubiquitinase that antagonizes the anaphase-promoting complex (APC/C) during G1/S transition by mediating deubiquitination of cyclin-A (CCNA1 and CCNA2), thereby promoting S phase entry. Specifically mediates deubiquitination of 'Lys-11'-linked polyubiquitin chains, a specific ubiquitin-linkage type mediated by the APC/C complex. Also mediates deubiquitination of 'Lys-48'-linked polyubiquitin chains in vitro. Phosphorylation at Ser-631 during G1/S phase maximizes the deubiquitinase activity, leading to prevent degradation of cyclin-A (CCNA1 and CCNA2) By similarity. |
| Catalytic activity | Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal). |
| Subunit structure | Interacts with FZR1/CDH1 By similarity. |
| Domain | The KEN box 3 is required for interaction with FZR1/CDH1 and is essential for APC(CDH1)-mediated ubiquitination By similarity. |
| Post-translational modification | Polyubiquitinated via 'Lys-11'-linked ubiquitin by the APC(CDH1) complex during late mitosis, leading to its degradation. Able to mediate auto-deubiquitination By similarity. Phosphorylated at Ser-631 by CDK2 during G1/S phase but not during mitosis; phosphorylation at Ser-631 is required for deubiquitinase activity. Also polyubiquitinated during early G1 phase, without leading to degradation By similarity. |
| Sequence similarities | Belongs to the peptidase C19 family. Contains 3 UIM (ubiquitin-interacting motif) repeats. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 982 | 982 | Ubiquitin carboxyl-terminal hydrolase 37 | PRO_0000412646 | |||||
Regions | |||||||||
| Repeat | 707 – 726 | 20 | UIM 1 | ||||||
| Repeat | 809 – 828 | 20 | UIM 2 | ||||||
| Repeat | 831 – 850 | 20 | UIM 3 | ||||||
| Motif | 32 – 34 | 3 | KEN box 1 By similarity | ||||||
| Motif | 71 – 79 | 9 | D-box 1 By similarity | ||||||
| Motif | 96 – 105 | 10 | D-box 2 By similarity | ||||||
| Motif | 161 – 169 | 9 | D-box 3 By similarity | ||||||
| Motif | 224 – 226 | 3 | KEN box 2 By similarity | ||||||
| Motif | 785 – 787 | 3 | KEN box 3 By similarity | ||||||
Sites | |||||||||
| Active site | 352 | 1 | Nucleophile By similarity | ||||||
| Active site | 909 | 1 | Proton acceptor By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 216 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 631 | 1 | Phosphoserine; by CDK2 By similarity | ||||||
| Modified residue | 653 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 655 | 1 | Phosphoserine By similarity | ||||||
Sequences
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References
| [1] | Swine Genome Sequencing Consortium. Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Landrace. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | CU570813 Genomic DNA. No translation available. |
3D structure databases | |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSSSCT00000017635; ENSSSCP00000017157; ENSSSCG00000016194. |
Phylogenomic databases | |
| GeneTree | ENSGT00440000033542. |
| OMA | LQEFNNS. |
Family and domain databases | |
| InterPro | IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. IPR003903. Ubiquitin-int_motif. [Graphical view] |
| Pfam | PF00443. UCH. 1 hit. PF02809. UIM. 3 hits. [Graphical view] |
| SMART | SM00726. UIM. 3 hits. [Graphical view] |
| PROSITE | PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. PS50330. UIM. 3 hits. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | UBP37_PIG | ||||||||
| Accession | Primary (citable) accession number: F1SRY5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |