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Protein

AP2-associated protein kinase 1

Gene

AAK1

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Enzyme regulationi

Stimulated by clathrin.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74ATPPROSITE-ProRule annotation1
Active sitei176Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi52 – 60ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processEndocytosis
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
AP2-associated protein kinase 11 Publication (EC:2.7.11.1By similarity)
Alternative name(s):
Adaptor-associated kinase 11 Publication
Gene namesi
Name:AAK1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Coated pit, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004133621 – 968AP2-associated protein kinase 1Add BLAST968

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei14PhosphoserineBy similarity1
Modified residuei234PhosphotyrosineBy similarity1
Modified residuei235PhosphoserineBy similarity1
Modified residuei354PhosphothreonineBy similarity1
Modified residuei389PhosphothreonineBy similarity1
Modified residuei391Omega-N-methylarginineBy similarity1
Modified residuei613PhosphothreonineBy similarity1
Modified residuei625PhosphoserineBy similarity1
Modified residuei627PhosphothreonineBy similarity1
Modified residuei630PhosphoserineBy similarity1
Modified residuei631PhosphoserineBy similarity1
Modified residuei644PhosphoserineBy similarity1
Modified residuei657PhosphoserineBy similarity1
Modified residuei660PhosphothreonineBy similarity1
Modified residuei694PhosphotyrosineBy similarity1
Modified residuei738PhosphoserineBy similarity1
Modified residuei853PhosphoserineBy similarity1
Modified residuei944PhosphoserineBy similarity1
Modified residuei945PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

PaxDbiF1SPM8.
PeptideAtlasiF1SPM8.
PRIDEiF1SPM8.

Interactioni

Subunit structurei

Interacts with alpha-adaptin, AP-2, clathrin, NUMB and EPS15. Interacts with membrane-bound activated NOTCH1 but not with the inactive full-length form of NOTCH1. Preferentially interacts with monoubiquitinated activated NOTCH1 compared to the non-ubiquitinated form (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000008900.

Structurei

3D structure databases

SMRiF1SPM8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 315Protein kinasePROSITE-ProRule annotationAdd BLAST270

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi397 – 621Gln-richAdd BLAST225
Compositional biasi665 – 670Poly-Ala6

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1989. Eukaryota.
ENOG410Y515. LUCA.
GeneTreeiENSGT00880000137958.
InParanoidiF1SPM8.

Family and domain databases

Gene3Di1.10.565.10. 1 hit.
InterProiView protein in InterPro
IPR011009. Kinase-like_dom_sf.
IPR035500. NHR_like_dom_sf.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

F1SPM8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKFFDSRRE QGGSGLGSGS SGGGGSTSGL GSGYIGRVFG IGRQQVTVDE
60 70 80 90 100
VLAEGGFAIV FLVRTSNGMK CALKRMFVNN EHDLQVCKRE IQIMRDLSGH
110 120 130 140 150
KNIVGYIDSS INNVSSGDVW EVLILMDFCR GGQVVNLMNQ RLQTGFTESE
160 170 180 190 200
VLQIFCDTCE AVARLHQCKT PIIHRDLKVE NILLHDRGHY VLCDFGSATN
210 220 230 240 250
KFQNPQTEGV NAVEDEIKKY TTLSYRAPEM VNLYSGKIIT TKADIWALGC
260 270 280 290 300
LLYKLCYFTL PFGESQVAIC DGNFTIPDNS RYSQDMHCLI RYMLEPDPDK
310 320 330 340 350
RPDIYQVSFF SFKLLKKECP VPNVQNSPIP AKLPEPVKAS EAAAKKTQPK
360 370 380 390 400
ARLTDPIPTT ETSIAPRQRP KAGQTQPNPG ILPIQPALTP RKRAMVQPPP
410 420 430 440 450
QVAGSSNQPG LLASVPQPKT QAPPSQPLPQ SQAKQPQAPP APQQPPSAPA
460 470 480 490 500
QGLPAQAQAT PQHQQQLFLK QQPQPPQPQP QAQAPPVKSL KFYPFYPMCK
510 520 530 540 550
GRQTVSSQFQ AVHPAAQQPA IAQFPAVSQG SSQQQLIQNF YQQQQQQQQQ
560 570 580 590 600
QQLATTLHQQ QLLTQQAALQ QKTTVAAIQP PQAQPATASQ PPPAQEPAQI
610 620 630 640 650
QAPVRQQPKV QTTPPPAIQG QKLGSLTPPS SPKAQRAGHR RILSDVTHSA
660 670 680 690 700
VFGVPASKST QLLQAAAAEA SLNKSKSATT TPSGSPRASQ QNVYNPSEGS
710 720 730 740 750
TWNPFDDDNF SKLTAEELLN KDFAKLGEGK HPEKLGGSAE SLIPGFQPTQ
760 770 780 790 800
GDAFAASSFA AGTAEKRKGG QAVDSSLPLL SVSDPFIPLQ VPDAPEKLIE
810 820 830 840 850
GLKSPDTSLL LPDLLPMTDP FGSTSDAVIE KADVAVESLI PGLEPPVPQR
860 870 880 890 900
LPSQTESVAS NRTDSLTGED ALIDCSLLSN PTTDLLEEFA PIAISAPAHK
910 920 930 940 950
AAEDSNLISG FDVPEGSDKV AEDEFDPIPV LITKNPQGGH SRNSSGSSES
960
SLPNLARSLL LVDQLIDL
Length:968
Mass (Da):104,310
Last modified:October 19, 2011 - v2
Checksum:i4F07200E056E047D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU467585 Genomic DNA. No translation available.
FS674854 mRNA. No translation available.

Genome annotation databases

EnsembliENSSSCT00000009130; ENSSSCP00000008900; ENSSSCG00000008337.

Similar proteinsi

Entry informationi

Entry nameiAAK1_PIG
AccessioniPrimary (citable) accession number: F1SPM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: October 19, 2011
Last modified: November 22, 2017
This is version 38 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families