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Protein

Deoxyhypusine hydroxylase

Gene

DOHH

Organism
Sus scrofa (Pig)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi56 – 561Iron 1UniRule annotation
Metal bindingi57 – 571Iron 1UniRule annotation
Metal bindingi89 – 891Iron 1UniRule annotation
Metal bindingi90 – 901Iron 1UniRule annotation
Metal bindingi208 – 2081Iron 2UniRule annotation
Metal bindingi209 – 2091Iron 2UniRule annotation
Metal bindingi241 – 2411Iron 2UniRule annotation
Metal bindingi242 – 2421Iron 2UniRule annotation

GO - Molecular functioni

  1. deoxyhypusine monooxygenase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. peptidyl-lysine modification to peptidyl-hypusine Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

MonooxygenaseUniRule annotation, Oxidoreductase

Keywords - Biological processi

Hypusine biosynthesisUniRule annotation

Keywords - Ligandi

IronUniRule annotation, Metal-bindingUniRule annotation

Enzyme and pathway databases

ReactomeiREACT_354680. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Gene namesi
Name:DOHHUniRule annotationImported
OrganismiSus scrofa (Pig)Imported
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Chromosome 2

Structurei

3D structure databases

ProteinModelPortaliF1S8E6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati176 – 19924HEAT-like PBS-typeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
Contains 1 HEAT-like PBS-type repeat.UniRule annotation

Phylogenomic databases

GeneTreeiENSGT00500000044957.
InParanoidiF1S8E6.
KOiK06072.
OMAiHEAAVPQ.
OrthoDBiEOG75TMCH.
TreeFamiTF105626.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
PfamiPF03130. HEAT_PBS. 1 hit.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

F1S8E6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTEQEVEAV GKMLVDPGQP LQARFRALFT LRGLGGPGAI TWISRAFGDD
60 70 80 90 100
SALLKHELAY CLGQMQDSRA IPVLVDVLRD TCQEPMVRHE AGEALGAIGN
110 120 130 140 150
PEVLEILKQY STDPVVEVAE TCQLAVRRLE WLQQHSGEPV AQGPYLSVDP
160 170 180 190 200
APPAQERDVG QLREMLLDEA QPLFDRYRAM FALRDIGGEE AALALAEGLL
210 220 230 240 250
CGSALFRHEI GYVLGQLQHE AAVPHLMAAL ARPAESPMVR HECAEALGAI
260 270 280 290 300
ARPACLAALR AYAADPERVV RESCEVALDM YEYETGPAFQ YADGLEQLRA

PPS
Length:303
Mass (Da):33,037
Last modified:May 3, 2011 - v1
Checksum:i5AF4AA0E1CB951D9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP016022 Genomic DNA. No translation available.
RefSeqiXP_003123090.1. XM_003123042.2.
XP_005654764.1. XM_005654707.1.

Genome annotation databases

EnsembliENSSSCT00000014724; ENSSSCP00000014326; ENSSSCG00000013478.
GeneIDi100519411.
KEGGissc:100519411.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FP016022 Genomic DNA. No translation available.
RefSeqiXP_003123090.1. XM_003123042.2.
XP_005654764.1. XM_005654707.1.

3D structure databases

ProteinModelPortaliF1S8E6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000014724; ENSSSCP00000014326; ENSSSCG00000013478.
GeneIDi100519411.
KEGGissc:100519411.

Organism-specific databases

CTDi83475.

Phylogenomic databases

GeneTreeiENSGT00500000044957.
InParanoidiF1S8E6.
KOiK06072.
OMAiHEAAVPQ.
OrthoDBiEOG75TMCH.
TreeFamiTF105626.

Enzyme and pathway databases

UniPathwayiUPA00354.
ReactomeiREACT_354680. Hypusine synthesis from eIF5A-lysine.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
PfamiPF03130. HEAT_PBS. 1 hit.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 6 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Porcine genome sequencing project
    Submitted (OCT-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. Ensembl
    Submitted (APR-2011) to UniProtKB
    Cited for: IDENTIFICATION.

Entry informationi

Entry nameiF1S8E6_PIG
AccessioniPrimary (citable) accession number: F1S8E6
Entry historyi
Integrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: May 3, 2011
Last modified: April 1, 2015
This is version 32 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.