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Protein

AP-2 complex subunit alpha

Gene

AP2A1

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosisUniRule annotation, Protein transportUniRule annotationSAAS annotation, Transport

Enzyme and pathway databases

ReactomeiR-CFA-177504. Retrograde neurotrophin signalling.
R-CFA-2132295. MHC class II antigen presentation.
R-CFA-3928665. EPH-ephrin mediated repulsion of cells.
R-CFA-416993. Trafficking of GluR2-containing AMPA receptors.
R-CFA-437239. Recycling pathway of L1.
R-CFA-5099900. WNT5A-dependent internalization of FZD4.
R-CFA-5140745. WNT5A-dependent internalization of FZD2, FZD5 and ROR2.
R-CFA-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-CFA-8856828. Clathrin-mediated endocytosis.
R-CFA-8866427. VLDLR internalisation and degradation.
R-CFA-8964038. LDL clearance.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit alphaUniRule annotation
Gene namesi
Name:AP2A1Imported
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)Imported
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 1

Subcellular locationi

Keywords - Cellular componenti

Coated pitUniRule annotation, Membrane

PTM / Processingi

Proteomic databases

PaxDbiF1PE67.
PRIDEiF1PE67.

Expressioni

Gene expression databases

BgeeiENSCAFG00000003541.

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000005278.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini746 – 859Alpha_adaptinC2InterPro annotationAdd BLAST114

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 43Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the adaptor complexes large subunit family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiKOG1077. Eukaryota.
ENOG410XNQE. LUCA.
GeneTreeiENSGT00550000074757.
InParanoidiF1PE67.
OMAiDFKTCIS.
OrthoDBiEOG091G01JZ.
TreeFamiTF300308.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiView protein in InterPro
IPR017104. AP2_complex_asu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR003164. Clathrin_a-adaptin_app_sub_C.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR013041. Clathrin_app_Ig-like_sf.
IPR009028. Coatomer/calthrin_app_sub_C.
PfamiView protein in Pfam
PF01602. Adaptin_N. 1 hit.
PF02296. Alpha_adaptin_C. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
PIRSFiPIRSF037091. AP2_complex_alpha. 1 hit.
SMARTiView protein in SMART
SM00809. Alpha_adaptinC2. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.

Sequencei

Sequence statusi: Complete.

F1PE67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL
60 70 80 90 100
DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV
110 120 130 140 150
LVNSNSELIR LINNAIKNDL ASRNPTFMCL ALHCIANVGS REMGEAFAAD
160 170 180 190 200
IPRILVAGDS MDSVKQSAAL CLLRLYKASP DLVPMGEWTA RVVHLLNDQH
210 220 230 240 250
MGVVTAAVSL ITCLCKKNPD DFKTCISLAV SRLSRIVSSA STDLQDYTYY
260 270 280 290 300
FVPAPWLSVK LLRLLQCYPP PEDAAVKGRL VECLETVLNK AQEPPKSKKV
310 320 330 340 350
QHSNAKNAIL FETISLIIHY DSEPNLLVRA CNQLGQFLQH RETNLRYLAL
360 370 380 390 400
ESMCTLASSE FSHEAVKTHI DTVINALKTE RDVSVRQRAA DLLYAMCDRS
410 420 430 440 450
NAKQIVSEML RYLETADYAI REEIVLKVAI LAEKYAVDYS WYVDTILNLI
460 470 480 490 500
RIAGDYVSEE VWYRVLQIVT NRDDVQGYAA KTVFEALQAP ACHENMVKVG
510 520 530 540 550
GYILGEFGNL IAGDPRSSPP VQFSLLHSKF HLCSVATRAL LLSTYIKFIN
560 570 580 590 600
LFPETKATIQ GVLRAGSQLR NADVELQQRA VEYLTLSSVA STDVLATVLE
610 620 630 640 650
EMPPFPERES SILAKLKRKK GPGAGSALDD SRRDPSSNDI NGGVEPTPST
660 670 680 690 700
VSTPSPSADL LGLRAAPPPA APPATSGAGN LLVDVFSDGP AAQPSLGPTP
710 720 730 740 750
EEAFLSELEP PAPESPMALL ADPAPAADPG PDDIGPPIPE ADELLNKFVC
760 770 780 790 800
KNNGVLFENQ LLQIGVKSEF RQNLGRMYLF YGNKTSVQFQ NFSPTVVHPG
810 820 830 840 850
DLQTQLAVQT KRVAAQVDGG AQVQQVLNIE CLRDFLTPPL LSVRFRYGGA
860 870 880 890 900
PQSLTLKLPV TINKFFQPTE MAAQDFFQRW KQLSLPQQEA QKIFKANHPM
910 920 930 940 950
IWPPLTAALL PQLLGFGSAL LDNVDPNPEN FVGAGIIQTK ALQVGCLLRL
960 970 980
EPNAQAQMYR LTLRTSKEPV SRHLCELLAQ QF
Length:982
Mass (Da):108,136
Last modified:October 31, 2012 - v2
Checksum:iDF5F0F21CFB74C4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEX03000795 Genomic DNA. No translation available.

Genome annotation databases

EnsembliENSCAFT00000005702; ENSCAFP00000005278; ENSCAFG00000003541.

Similar proteinsi

Entry informationi

Entry nameiF1PE67_CANLF
AccessioniPrimary (citable) accession number: F1PE67
Entry historyiIntegrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: October 31, 2012
Last modified: January 31, 2018
This is version 45 of the entry and version 2 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Caution

The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.Imported

Keywords - Technical termi

Complete proteome, Reference proteomeImported