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Protein

AP-2 complex subunit alpha

Gene

AP2A1

Organism
Canis lupus familiaris (Dog) (Canis familiaris)
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha subunit binds polyphosphoinositide-containing lipids, positioning AP-2 on the membrane. The AP-2 alpha subunit acts via its C-terminal appendage domain as a scaffolding platform for endocytic accessory proteins. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei43Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei53Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei57Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei58Phosphatidylinositol lipid headgroupUniRule annotation1
Binding sitei61Phosphatidylinositol lipid headgroupUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processEndocytosisUniRule annotation, Protein transportUniRule annotationSAAS annotation, Transport

Enzyme and pathway databases

ReactomeiR-CFA-177504 Retrograde neurotrophin signalling
R-CFA-2132295 MHC class II antigen presentation
R-CFA-3928665 EPH-ephrin mediated repulsion of cells
R-CFA-416993 Trafficking of GluR2-containing AMPA receptors
R-CFA-437239 Recycling pathway of L1
R-CFA-5099900 WNT5A-dependent internalization of FZD4
R-CFA-5140745 WNT5A-dependent internalization of FZD2, FZD5 and ROR2
R-CFA-8856825 Cargo recognition for clathrin-mediated endocytosis
R-CFA-8856828 Clathrin-mediated endocytosis
R-CFA-8866427 VLDLR internalisation and degradation
R-CFA-8964038 LDL clearance

Names & Taxonomyi

Protein namesi
Recommended name:
AP-2 complex subunit alphaUniRule annotation
Gene namesi
Name:AP2A1Imported
OrganismiCanis lupus familiaris (Dog) (Canis familiaris)Imported
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
Proteomesi
  • UP000002254 Componenti: Chromosome 1

Organism-specific databases

VGNCiVGNC:37958 AP2A1

Subcellular locationi

Keywords - Cellular componenti

Coated pitUniRule annotation, Membrane

PTM / Processingi

Proteomic databases

PaxDbiF1PE67
PRIDEiF1PE67

Expressioni

Gene expression databases

BgeeiENSCAFG00000003541

Interactioni

Subunit structurei

Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).UniRule annotation

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9615.ENSCAFP00000005278

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini746 – 859Alpha_adaptinC2InterPro annotationAdd BLAST114

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili23 – 43Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the adaptor complexes large subunit family.UniRule annotation

Keywords - Domaini

Coiled coilSequence analysis

Phylogenomic databases

eggNOGiKOG1077 Eukaryota
ENOG410XNQE LUCA
GeneTreeiENSGT00550000074757
InParanoidiF1PE67
OMAiHNELFNC
OrthoDBiEOG091G01JZ
TreeFamiTF300308

Family and domain databases

Gene3Di1.25.10.10, 1 hit
3.30.310.10, 1 hit
InterProiView protein in InterPro
IPR017104 AP2_complex_asu
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR002553 Clathrin/coatomer_adapt-like_N
IPR003164 Clathrin_a-adaptin_app_sub_C
IPR008152 Clathrin_a/b/g-adaptin_app_Ig
IPR013041 Clathrin_app_Ig-like_sf
IPR009028 Coatomer/calthrin_app_sub_C
IPR012295 TBP_dom_sf
PfamiView protein in Pfam
PF01602 Adaptin_N, 1 hit
PF02296 Alpha_adaptin_C, 1 hit
PF02883 Alpha_adaptinC2, 1 hit
PIRSFiPIRSF037091 AP2_complex_alpha, 1 hit
SMARTiView protein in SMART
SM00809 Alpha_adaptinC2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
SSF49348 SSF49348, 1 hit
SSF55711 SSF55711, 1 hit

Sequencei

Sequence statusi: Complete.

F1PE67-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL
60 70 80 90 100
DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV
110 120 130 140 150
LVNSNSELIR LINNAIKNDL ASRNPTFMCL ALHCIANVGS REMGEAFAAD
160 170 180 190 200
IPRILVAGDS MDSVKQSAAL CLLRLYKASP DLVPMGEWTA RVVHLLNDQH
210 220 230 240 250
MGVVTAAVSL ITCLCKKNPD DFKTCISLAV SRLSRIVSSA STDLQDYTYY
260 270 280 290 300
FVPAPWLSVK LLRLLQCYPP PEDAAVKGRL VECLETVLNK AQEPPKSKKV
310 320 330 340 350
QHSNAKNAIL FETISLIIHY DSEPNLLVRA CNQLGQFLQH RETNLRYLAL
360 370 380 390 400
ESMCTLASSE FSHEAVKTHI DTVINALKTE RDVSVRQRAA DLLYAMCDRS
410 420 430 440 450
NAKQIVSEML RYLETADYAI REEIVLKVAI LAEKYAVDYS WYVDTILNLI
460 470 480 490 500
RIAGDYVSEE VWYRVLQIVT NRDDVQGYAA KTVFEALQAP ACHENMVKVG
510 520 530 540 550
GYILGEFGNL IAGDPRSSPP VQFSLLHSKF HLCSVATRAL LLSTYIKFIN
560 570 580 590 600
LFPETKATIQ GVLRAGSQLR NADVELQQRA VEYLTLSSVA STDVLATVLE
610 620 630 640 650
EMPPFPERES SILAKLKRKK GPGAGSALDD SRRDPSSNDI NGGVEPTPST
660 670 680 690 700
VSTPSPSADL LGLRAAPPPA APPATSGAGN LLVDVFSDGP AAQPSLGPTP
710 720 730 740 750
EEAFLSELEP PAPESPMALL ADPAPAADPG PDDIGPPIPE ADELLNKFVC
760 770 780 790 800
KNNGVLFENQ LLQIGVKSEF RQNLGRMYLF YGNKTSVQFQ NFSPTVVHPG
810 820 830 840 850
DLQTQLAVQT KRVAAQVDGG AQVQQVLNIE CLRDFLTPPL LSVRFRYGGA
860 870 880 890 900
PQSLTLKLPV TINKFFQPTE MAAQDFFQRW KQLSLPQQEA QKIFKANHPM
910 920 930 940 950
IWPPLTAALL PQLLGFGSAL LDNVDPNPEN FVGAGIIQTK ALQVGCLLRL
960 970 980
EPNAQAQMYR LTLRTSKEPV SRHLCELLAQ QF
Length:982
Mass (Da):108,136
Last modified:October 31, 2012 - v2
Checksum:iDF5F0F21CFB74C4E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AAEX03000795 Genomic DNA No translation available.

Genome annotation databases

EnsembliENSCAFT00000005702; ENSCAFP00000005278; ENSCAFG00000003541

Similar proteinsi

Entry informationi

Entry nameiF1PE67_CANLF
AccessioniPrimary (citable) accession number: F1PE67
Entry historyiIntegrated into UniProtKB/TrEMBL: May 3, 2011
Last sequence update: October 31, 2012
Last modified: May 23, 2018
This is version 48 of the entry and version 2 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

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