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Protein

Receptor-type tyrosine-protein phosphatase S

Gene

PTPRS

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell surface receptor that binds to glycosaminoglycans, including chondroitin sulfate proteoglycans and heparan sulfate proteoglycans (PubMed:11865065, PubMed:17178832). Binding to chondroitin sulfate and heparan sulfate proteoglycans has opposite effects on PTPRS oligomerization and regulation of neurite outgrowth (By similarity). Contributes to the inhibition of neurite and axonal outgrowth by chondroitin sulfate proteoglycans, also after nerve transection (PubMed:17967490). Plays a role in stimulating neurite outgrowth in response to the heparan sulfate proteoglycan GPC2. Required for normal brain development, especially for normal development of the pituitary gland and the olfactory bulb. Functions as tyrosine phosphatase (PubMed:17967490). Mediates dephosphorylation of NTRK1, NTRK2 and NTRK3 (PubMed:17967490). Plays a role in down-regulation of signaling cascades that lead to the activation of Akt and MAP kinases. Down-regulates TLR9-mediated activation of NF-kappa-B, as well as production of TNF, interferon alpha and interferon beta (By similarity).By similarity3 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1157Phosphocysteine intermediatePROSITE-ProRule annotation1
Active sitei1448Phosphocysteine intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • heparan sulfate proteoglycan binding Source: UniProtKB
  • heparin binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB
  • protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHeparin-binding, Hydrolase, Protein phosphatase, Receptor

Enzyme and pathway databases

ReactomeiR-GGA-3000178. ECM proteoglycans.

Names & Taxonomyi

Protein namesi
Recommended name:
Receptor-type tyrosine-protein phosphatase S (EC:3.1.3.48By similarity)
Short name:
R-PTP-S
Alternative name(s):
Chick receptor tyrosine phosphatase alpha1 Publication
Short name:
CRYP alpha1 Publication
Short name:
CRYPalpha1 Publication
Gene namesi
Name:PTPRSImported
OrganismiGallus gallus (Chicken)Imported
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componentsi: Chromosome 28, Unassembled WGS sequence

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 854ExtracellularSequence analysisAdd BLAST826
Transmembranei855 – 875HelicalSequence analysisAdd BLAST21
Topological domaini876 – 1516CytoplasmicSequence analysisAdd BLAST641

GO - Cellular componenti

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasmic vesicle, Membrane, Postsynaptic cell membrane, Synapse, Synaptosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67 – 71KKGKK → AAGAA: Loss of heparin binding. 1 Publication5
Mutagenesisi96R → A: Loss of heparin binding; when associated with A-99. 1 Publication1
Mutagenesisi99R → A: Loss of heparin binding; when associated with A-96. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_500895416229 – 1516Receptor-type tyrosine-protein phosphatase SAdd BLAST1488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi53 ↔ 106PROSITE-ProRule annotation
Disulfide bondi155 ↔ 206PROSITE-ProRule annotation
Glycosylationi253N-linked (GlcNAc...)PROSITE-ProRule annotation1
Disulfide bondi256 ↔ 301PROSITE-ProRule annotation
Glycosylationi298N-linked (GlcNAc...)PROSITE-ProRule annotation1

Post-translational modificationi

A cleavage occurs, separating the extracellular domain from the transmembrane segment. This process called 'ectodomain shedding' is thought to be involved in receptor desensitization, signal transduction and/or membrane localization (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ90815.

Expressioni

Tissue specificityi

Detected in embryonic brain, dorsal root ganglion and spinal cord (PubMed:7600997). Detected in embryonic retina (at protein level) (PubMed:11865065). Detected in embryonic brain, spinal cord, dorsal root ganglion, trigeminal ganglion, ganglia associated with the precardinal vein and vagus nerve, the inner and outer nuclear layer of the retina, limb, breast muscle, heart, gut and lung.3 Publications

Gene expression databases

BgeeiENSGALG00000004048.

Interactioni

Subunit structurei

Homodimer (PubMed:17178832). Binding to large heparan sulfate proteoglycan structures promotes oligomerization. Binding to chondroitin sulfate proteoglycan does not lead to oligomerization (By similarity). Interacts (via Ig-like domains) with NTRK1 and NTRK3, but does not form detectable complexes with NTRK2 (PubMed:17967490, PubMed:25385546). Interacts (via extracellular domain) with the heparan sulfate proteoglycans AGRN and COL18A1 (PubMed:11865065).By similarity4 Publications

GO - Molecular functioni

  • heparan sulfate proteoglycan binding Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YD4X-ray1.65A29-226[»]
4PBVX-ray2.50C/D/E29-316[»]
4PBWX-ray3.05D/E/F29-316[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini32 – 122Ig-like C2-type 1PROSITE-ProRule annotationAdd BLAST91
Domaini134 – 223Ig-like C2-type 2PROSITE-ProRule annotationAdd BLAST90
Domaini235 – 317Ig-like C2-type 3PROSITE-ProRule annotationAdd BLAST83
Domaini324 – 414Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST91
Domaini419 – 513Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST95
Domaini517 – 606Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST90
Domaini608 – 692Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST85
Domaini961 – 1216Tyrosine-protein phosphatase 1PROSITE-ProRule annotationAdd BLAST256
Domaini1248 – 1507Tyrosine-protein phosphatase 2PROSITE-ProRule annotationAdd BLAST260

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 71Important for binding to glycosaminoglycan chains1 Publication5

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00760000118900.
HOGENOMiHOG000010250.
HOVERGENiHBG053758.
OMAiGGTERCG.
OrthoDBiEOG091G11WG.

Family and domain databases

CDDicd00063. FN3. 4 hits.
Gene3Di2.60.40.10. 8 hits.
3.90.190.10. 2 hits.
InterProiView protein in InterPro
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
PfamiView protein in Pfam
PF00041. fn3. 3 hits.
PF07679. I-set. 2 hits.
PF00102. Y_phosphatase. 2 hits.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiView protein in SMART
SM00060. FN3. 4 hits.
SM00409. IG. 3 hits.
SM00408. IGc2. 3 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
SUPFAMiSSF48726. SSF48726. 3 hits.
SSF49265. SSF49265. 2 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiView protein in PROSITE
PS50853. FN3. 4 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: F1NWE3-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRILPSPGMP ALLSLVSLLS VLLMGCVAES PPVFIKKPVD QIGVSGGVAS
60 70 80 90 100
FVCQATGDPK PRVTWNKKGK KVNSQRFETI EFDESAGAVL RIQPLRTPRD
110 120 130 140 150
ENIYECVAQN PHGEVTVHAK LTVLREDQLP PGFPNIDMGP QLKVVERTRT
160 170 180 190 200
ATMLCAASGN PDPEITWFKD FLPVDPSTSN GRIKQLRSGG LQIESSEETD
210 220 230 240 250
QGKYECVASN SAGVRYSSPA NLYVRELREV RRVAPRFSIL PVSHEIMPGG
260 270 280 290 300
NVNITCVAVG SPMPYVKWMQ GAEDLTPEDD MPVGRNVLEL TDVKDSANYT
310 320 330 340 350
CVAMSSLGVI EAVAQITVKS LPKAPGTPVV TETTATSITI TWDSGNPDPV
360 370 380 390 400
SYYVIEYKSK SQDGPYQIKE DITTTRYSIG GLSPNSEYEI WVSAVNSIGQ
410 420 430 440 450
GPPSESVVTR TGEQAPASAP RNVQGRMLSS TTMIIQWEEP VEPNGQIRGY
460 470 480 490 500
RVYYTMEPDQ PVSNWQKHNV DDSLLTTVGS LLEDETYTVR VLAFTSVGDG
510 520 530 540 550
PLSDPIQVKT QQGVPGQPMN FRAEAKTETS IVLSWSPPRQ EIIVKYELLF
560 570 580 590 600
KEGDHGREVP RNFEPTTSFT VEGLKPNTEY VFRLAARSAL GLGAFTPEVR
610 620 630 640 650
ERTLQSILPK NFKVKMVTKT SVLLSWEFPE NYNSPTPYKI QYNGLNVDVD
660 670 680 690 700
GRTTKKLITN LKPHTFYNFV LMNRGNSMGG LQQNVAAWTA ANMLSRKPEV
710 720 730 740 750
THKPDADGNV VVILPDVKSS VAVQAYYIVV VPLRKSRGGQ FLNPLGSPEE
760 770 780 790 800
MDLEELIQDI ARLRRRSLRH SRQLDFPKPY IAARFRSLPN HFVLGDMKHY
810 820 830 840 850
DNFENRALEP GQRYVIFILA VLQEPEATFA ASPFSDPIQL DNPDPQPIID
860 870 880 890 900
GEEGLIWVIG PVLAVVFIIC IVIAILLYKN KRKDSEPRTK CLLNNAEITP
910 920 930 940 950
HHPKDPVEMR RINFQTPDSG LSSPLSDPEF DFESMLSHPP IPVSELAEHT
960 970 980 990 1000
EHLKANDNLK LSQEYESIDP GQQFTWEHSN LEVNKPKNRY ANVIAYDHSR
1010 1020 1030 1040 1050
VILLPIEGIV GSDYINANYI DGYRKQNAYI ATQGPLPETF GDFWRMVWEQ
1060 1070 1080 1090 1100
RSATIVMMTK LEEKSRIKCD QYWPGRGTDT YGMIQVTLLD TIELATFCVR
1110 1120 1130 1140 1150
TFSLHKNGSS EKREVRQFQF TAWPDHGVPE YPTPFLAFLR RVKTCNPPDA
1160 1170 1180 1190 1200
GPIVVHCSAG VGRTGCFIVI DAMLERIKHE KTVDIYGHVT LMRSQRNYMV
1210 1220 1230 1240 1250
QTEDQYSFIH DALLEAVACG NTEVPARNLY TYIQKLAQIE VGEHVTGMEL
1260 1270 1280 1290 1300
EFKRLANSKA HTSRFISANL PCNKFKNRLV NIMPYETTRV CLQPIRGVEG
1310 1320 1330 1340 1350
SDYINASFID GYRQQKAYIA TQGPLAETTE DFWRMLWEHN STIVVMLTKL
1360 1370 1380 1390 1400
REMGREKCHQ YWPAERSARY QYFVVDPMAE YNMPQYILRE FKVTDARDGQ
1410 1420 1430 1440 1450
SRTVRQFQFT DWPEQGVPKS GEGFIDFIGQ VHKTKEQFGQ DGPISVHCSA
1460 1470 1480 1490 1500
GVGRTGVFIT LSIVLERMRY EGVVDIFQTV KMLRTQRPAM VQTEDEYQFC
1510
YQAALEYLGS FDHYAT
Length:1,516
Mass (Da):170,604
Last modified:November 30, 2016 - v3
Checksum:iF577A09B7CA138C0
GO
Isoform 2 (identifier: F1NWE3-2) [UniParc]FASTAAdd to basket
Also known as: CRYPalpha11 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     226-229: Missing.
     880-880: N → NKPDS
     918-934: DSGLSSPLSDPEFDFES → G

Show »
Length:1,500
Mass (Da):168,750
Checksum:iEFE2E031265AFFB9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1339H → N in AAA64460 (PubMed:7918104).Curated1
Sequence conflicti1487Missing in AAA64460 (PubMed:7918104).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_058832226 – 229Missing in isoform 2. 4
Alternative sequenceiVSP_058833880N → NKPDS in isoform 2. 1
Alternative sequenceiVSP_058834918 – 934DSGLS…FDFES → G in isoform 2. Add BLAST17

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32780 mRNA. Translation: AAA64460.1.
AADN04000371 Genomic DNA. No translation available.
PIRiI50212.

Genome annotation databases

EnsembliENSGALT00000006443; ENSGALP00000006433; ENSGALG00000004048.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32780 mRNA. Translation: AAA64460.1.
AADN04000371 Genomic DNA. No translation available.
PIRiI50212.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YD4X-ray1.65A29-226[»]
4PBVX-ray2.50C/D/E29-316[»]
4PBWX-ray3.05D/E/F29-316[»]
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ90815.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000006443; ENSGALP00000006433; ENSGALG00000004048.

Phylogenomic databases

GeneTreeiENSGT00760000118900.
HOGENOMiHOG000010250.
HOVERGENiHBG053758.
OMAiGGTERCG.
OrthoDBiEOG091G11WG.

Enzyme and pathway databases

ReactomeiR-GGA-3000178. ECM proteoglycans.

Gene expression databases

BgeeiENSGALG00000004048.

Family and domain databases

CDDicd00063. FN3. 4 hits.
Gene3Di2.60.40.10. 8 hits.
3.90.190.10. 2 hits.
InterProiView protein in InterPro
IPR003961. FN3_dom.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000242. PTPase_domain.
IPR016130. Tyr_Pase_AS.
IPR003595. Tyr_Pase_cat.
IPR000387. TYR_PHOSPHATASE_dom.
PfamiView protein in Pfam
PF00041. fn3. 3 hits.
PF07679. I-set. 2 hits.
PF00102. Y_phosphatase. 2 hits.
PRINTSiPR00700. PRTYPHPHTASE.
SMARTiView protein in SMART
SM00060. FN3. 4 hits.
SM00409. IG. 3 hits.
SM00408. IGc2. 3 hits.
SM00194. PTPc. 2 hits.
SM00404. PTPc_motif. 2 hits.
SUPFAMiSSF48726. SSF48726. 3 hits.
SSF49265. SSF49265. 2 hits.
SSF52799. SSF52799. 2 hits.
PROSITEiView protein in PROSITE
PS50853. FN3. 4 hits.
PS50835. IG_LIKE. 3 hits.
PS00383. TYR_PHOSPHATASE_1. 2 hits.
PS50056. TYR_PHOSPHATASE_2. 2 hits.
PS50055. TYR_PHOSPHATASE_PTP. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiPTPRS_CHICK
AccessioniPrimary (citable) accession number: F1NWE3
Secondary accession number(s): Q90815
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2017
Last sequence update: November 30, 2016
Last modified: April 12, 2017
This is version 50 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.