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Protein

Multiple inositol polyphosphate phosphatase 1

Gene

MINPP1

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as a phosphoinositide 5- and phosphoinositide 6-phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6) (PubMed:9472008). Also acts as a 2,3-bisphosphoglycerate 3-phosphatase, by mediating the dephosphorylation of 2,3-bisphosphoglycerate (2,3-BPG) to produce phospho-D-glycerate without formation of 3-phosphoglycerate (By similarity). May play a role in bone development (endochondral ossification) (By similarity). May play a role in the transition of chondrocytes from proliferation to hypertrophy (PubMed:8660956, PubMed:9472008).2 PublicationsBy similarity1 Publication

Miscellaneous

Monogastric animals cannot hydrolyze dietary inositol hexakisphosphate (InsP6) which makes up the bulk of organic phosphates in soybean, grains and other plant seeds which are the primary source of animal feed. The high activity of the chicken protein toward InsP6 offers a genetic strategy for improving InsP6 digestion in poultry and reducing the pollution that results from high phosphate content in manure.1 Publication

Catalytic activityi

Myo-inositol hexakisphosphate + H2O = myo-inositol pentakisphosphate (mixed isomers) + phosphate.1 Publication
2,3-bisphospho-D-glycerate + H2O = 2-phospho-D-glycerate + phosphate.By similarity

Kineticsi

  1. KM=140 µM for inositol hexakisphosphate1 Publication
  1. Vmax=715 nmol/min/mg enzyme with inositol hexakisphosphate as substrate1 Publication

pH dependencei

Optimum pH is 4.5-7.5.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei69By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Multiple inositol polyphosphate phosphatase 1By similarity (EC:3.1.3.621 Publication)
Alternative name(s):
2,3-bisphosphoglycerate 3-phosphataseBy similarity (EC:3.1.3.80By similarity)
Short name:
2,3-BPG phosphataseBy similarity
Band 171 Publication
Histidine phosphatase of the endoplasmic reticulum 11 Publication
Short name:
HiPER11 Publication
Gene namesi
Name:MINPP1
OrganismiGallus gallus (Chicken)Imported
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27T → G: Loss of 96% of activity towards InsP6. 1 Publication1
Mutagenesisi78Q → A: Two-fold greater substrate affinity and doubling of catalytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000043714020 – 448Multiple inositol polyphosphate phosphatase 1Sequence analysisAdd BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi203N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi257N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi409N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi441N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiF1NPQ2

Expressioni

Tissue specificityi

Present in growth plate chondrocytes but not detectable in articular chondrocytes (at protein level) (PubMed:9472008). Spatially restricted to chondrocytes in the lower portion of the proliferative zone and the upper portion of the hypertrophic zone in the growth plate of long bones (at protein level) (PubMed:9472008). Weakly expressed in kidney, liver, lung, skin and spleen, and not detected in brain, heart and muscle (PubMed:8660956).2 Publications

Inductioni

Repressed by parathyroid hormone-related protein PTHLH/PTHRP.1 Publication

Gene expression databases

BgeeiENSGALG00000003695

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000005849

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi445 – 448Prevents secretion from ERPROSITE-ProRule annotation4

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1382 Eukaryota
ENOG4111FS8 LUCA
HOGENOMiHOG000113591
HOVERGENiHBG052872
KOiK03103
OrthoDBiEOG091G0FRE

Family and domain databases

CDDicd07061 HP_HAP_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR000560 His_Pase_clade-2
IPR029033 His_PPase_superfam
IPR016274 Histidine_acid_Pase_euk
PfamiView protein in Pfam
PF00328 His_Phos_2, 1 hit
PIRSFiPIRSF000894 Acid_phosphatase, 1 hit
SUPFAMiSSF53254 SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00014 ER_TARGET, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F1NPQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPRRAACLL PLLVAVASAG LGGYFGTKSR YEEVNPHLAE DPLSLGPHAA
60 70 80 90 100
ASRLPAACAP LQLRAVLRHG TRYPTAGQIR RLAELHGRLR RAAAPSCPAA
110 120 130 140 150
AALAAWPMWY EESLDRLAPR GRRDMEHLAR RLAARFPALF AARRRLALAS
160 170 180 190 200
SSKHRCLQSG AAFRRGLGPS LSLGADETEI EVNDALMRFF DHCDKFVAFV
210 220 230 240 250
EDNDTAMYQV NAFKEGPEMR KVLEKVASAL CLPASELNAD LVQVAFLTCS
260 270 280 290 300
YELAIKNVTS PWCSLFSEED AKVLEYLNDL KQYWKRGYGY DINSRSSCIL
310 320 330 340 350
FQDIFQQLDK AVDESRSSKP ISSPLIVQVG HAETLQPLLA LMGYFKDAEP
360 370 380 390 400
LQANNYIRQA HRKFRSGRIV PYAANLVFVL YHCEQKTSKE EYQVQMLLNE
410 420 430 440
KPMLFHHSNE TISTYADLKS YYKDILQNCH FEEVCELPKV NGTVADEL
Length:448
Mass (Da):50,200
Last modified:September 7, 2016 - v3
Checksum:iD62BA17068ECD5BA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4R → C in AAB97100 (PubMed:8660956).Curated1
Sequence conflicti52S → A in AAB97100 (PubMed:8660956).Curated1
Sequence conflicti65 – 67AVL → RVV in AAB97100 (PubMed:8660956).Curated3
Sequence conflicti115D → DG in AAB97100 (PubMed:8660956).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U59421 mRNA Translation: AAB97100.1
AADN03005023 Genomic DNA No translation available.
RefSeqiNP_989975.1, NM_204644.1
UniGeneiGga.3975

Genome annotation databases

GeneIDi395356
KEGGigga:395356

Similar proteinsi

Entry informationi

Entry nameiMINP1_CHICK
AccessioniPrimary (citable) accession number: F1NPQ2
Secondary accession number(s): Q92170
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 7, 2016
Last sequence update: September 7, 2016
Last modified: June 7, 2017
This is version 40 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health