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F1NPG5

- CENPT_CHICK

UniProt

F1NPG5 - CENPT_CHICK

Protein

Centromere protein T

Gene

CENPT

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 27 (01 Oct 2014)
      Sequence version 2 (16 May 2012)
      Previous versions | rss
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    Functioni

    Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation By similarity. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres By similarity. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.By similarity2 Publications

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. protein binding Source: IntAct

    GO - Biological processi

    1. chromosome segregation Source: Ensembl
    2. kinetochore assembly Source: InterPro
    3. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198182. Resolution of Sister Chromatid Cohesion.
    REACT_210486. Separation of Sister Chromatids.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Centromere protein T
    Short name:
    CENP-T
    Gene namesi
    Name:CENPT
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Unplaced

    Subcellular locationi

    Nucleus. Chromosomecentromerekinetochore
    Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus.

    GO - Cellular componenti

    1. condensed chromosome kinetochore Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Centromere, Chromosome, Kinetochore, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 639639Centromere protein TPRO_0000417383Add
    BLAST

    Interactioni

    Subunit structurei

    Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU By similarity. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS By similarity. Part of a centromere complex consisting of CENPA, CENPT and CENPW By similarity. Part of a centromere complex consisting of histone H3, CENPT and CENPW. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPW. Interacts (via N-terminus) with the NDC80 complex. Binds DNA.By similarity3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APITD1E1BSW73EBI-2132248,EBI-5487792
    CENPWP0DJH64EBI-2132248,EBI-2132287

    Protein-protein interaction databases

    IntActiF1NPG5. 4 interactions.

    Structurei

    Secondary structure

    1
    639
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi66 – 705
    Helixi73 – 8311
    Helixi538 – 54912
    Helixi555 – 58228
    Beta strandi586 – 5883
    Helixi590 – 59910
    Beta strandi602 – 6043
    Beta strandi605 – 6073
    Helixi609 – 6168
    Helixi619 – 6257

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3B0CX-ray2.20T531-639[»]
    3B0DX-ray2.20B/T531-639[»]
    3VH5X-ray2.40T531-637[»]
    3VH6X-ray3.35T531-637[»]
    3VZAX-ray1.90E/F63-98[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni80 – 500421Flexible stalk domainAdd
    BLAST

    Domaini

    The largest part of the sequence forms an elongated and flexible stalk structure that is connected to a C-terminal globular domain with a histone-type fold.1 Publication

    Sequence similaritiesi

    Belongs to the CENPT family.Curated

    Phylogenomic databases

    KOiK11512.

    Family and domain databases

    Gene3Di1.10.20.10. 1 hit.
    InterProiIPR028255. CENP-T.
    IPR009072. Histone-fold.
    [Graphical view]
    PANTHERiPTHR14857. PTHR14857. 1 hit.
    SUPFAMiSSF47113. SSF47113. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    F1NPG5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDGRVGLRAS RRAAPTPRVA VRSSPRHRAR VREQEPVVSA MSGSGLGKEN    50
    KAGSSTPLRH RPNAFSELDN ATPRVMLRKI IQNQPQVSPL ALQTVQLEET 100
    EDARPEPPSQ RTSSTVELQL PDLVPEDASV TTFRMTRKRK KLSISEFERA 150
    ADKRLPQNQA HSTLDSTLVR SLRMSVGSVM APDTVEKRGL LRRPQNHKAI 200
    DIAAFEGGVE QNMLQIKAQD YLVDLQTSSM TGTTTIRTDA EVVLNNTELF 250
    VEPQLGEQNL LAVEPQLSDS KTSAQRSNTS YPAHEKARLE GLVSRVSTDE 300
    RRTLRFSEKD LITDHEHVDG ITQKTPAKQG EEEQDHSQQN DPMEQFSESE 350
    EMAGTTEHHA DAEYSEHSEK KLSRKAVSQL TAAQDAGVEM EMTPSEGGVA 400
    EGTEHQDSPK AELQMAGSPG GHSPASYSLE KPGAKPLKEA VEQTGEIERG 450
    TITGVLDAAE EEATDDESDK EDHESEEISM KTPMFVHAAA YRPQPVLSPP 500
    HPVKSASPEL PPQPVRAKPV PKSSGAAQRK TREPEIASSL IKQIFSHYVK 550
    TPVTRDAYKI VEKCSERYFK QISSDLEAYS QHAGRKTVEM ADVELLMRRQ 600
    GLVTDKMPLH VLVERHLPLE YRKLLIPIAV SGNKVIPCK 639
    Length:639
    Mass (Da):70,670
    Last modified:May 16, 2012 - v2
    Checksum:i9D314920A56ADC11
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ452155 mRNA. No translation available.
    BU128959 mRNA. No translation available.
    BU260128 mRNA. No translation available.
    BU381659 mRNA. No translation available.
    BU382952 mRNA. No translation available.
    BU433983 mRNA. No translation available.
    CD734856 mRNA. No translation available.
    AADN02051672 Genomic DNA. No translation available.
    RefSeqiNP_001263242.1. NM_001276313.1.
    UniGeneiGga.2263.
    Gga.54685.

    Genome annotation databases

    GeneIDi415654.
    KEGGigga:415654.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ452155 mRNA. No translation available.
    BU128959 mRNA. No translation available.
    BU260128 mRNA. No translation available.
    BU381659 mRNA. No translation available.
    BU382952 mRNA. No translation available.
    BU433983 mRNA. No translation available.
    CD734856 mRNA. No translation available.
    AADN02051672 Genomic DNA. No translation available.
    RefSeqi NP_001263242.1. NM_001276313.1.
    UniGenei Gga.2263.
    Gga.54685.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3B0C X-ray 2.20 T 531-639 [» ]
    3B0D X-ray 2.20 B/T 531-639 [» ]
    3VH5 X-ray 2.40 T 531-637 [» ]
    3VH6 X-ray 3.35 T 531-637 [» ]
    3VZA X-ray 1.90 E/F 63-98 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi F1NPG5. 4 interactions.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 415654.
    KEGGi gga:415654.

    Organism-specific databases

    CTDi 80152.

    Phylogenomic databases

    KOi K11512.

    Enzyme and pathway databases

    Reactomei REACT_198182. Resolution of Sister Chromatid Cohesion.
    REACT_210486. Separation of Sister Chromatids.

    Family and domain databases

    Gene3Di 1.10.20.10. 1 hit.
    InterProi IPR028255. CENP-T.
    IPR009072. Histone-fold.
    [Graphical view ]
    PANTHERi PTHR14857. PTHR14857. 1 hit.
    SUPFAMi SSF47113. SSF47113. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: White Leghorn Hisex.
    2. "Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution."
      Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A.
      , Kremitzki C., Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D., Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K., Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E., Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M., Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S., Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M., Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O., Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R., Wilson R.K.
      Nature 432:695-716(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Red jungle fowl.
    3. "CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore."
      Hori T., Amano M., Suzuki A., Backer C.B., Welburn J.P., Dong Y., McEwen B.F., Shang W.-H., Suzuki E., Okawa K., Cheeseman I.M., Fukagawa T.
      Cell 135:1039-1052(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    4. "Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins."
      Suzuki A., Hori T., Nishino T., Usukura J., Miyagi A., Morikawa K., Fukagawa T.
      J. Cell Biol. 193:125-140(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH CENPW.
    5. "CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."
      Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
      Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 54-162, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiCENPT_CHICK
    AccessioniPrimary (citable) accession number: F1NPG5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 16, 2012
    Last sequence update: May 16, 2012
    Last modified: October 1, 2014
    This is version 27 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3