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F1NPG5 (CENPT_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 24. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Centromere protein T

Short name=CENP-T
Gene names
Name:CENPT
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation By similarity. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres By similarity. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis. Ref.3 Ref.5

Subunit structure

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU By similarity. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS By similarity. Part of a centromere complex consisting of CENPA, CENPT and CENPW By similarity. Part of a centromere complex consisting of histone H3, CENPT and CENPW. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPW. Interacts (via N-terminus) with the NDC80 complex. Binds DNA. Ref.3 Ref.4 Ref.5

Subcellular location

Nucleus. Chromosomecentromerekinetochore. Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus. Ref.3 Ref.4 Ref.5

Domain

The largest part of the sequence forms an elongated and flexible stalk structure that is connected to a C-terminal globular domain with a histone-type fold. Ref.4

Miscellaneous

Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.

Sequence similarities

Belongs to the CENPT family.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentCentromere
Chromosome
Kinetochore
Nucleus
   LigandDNA-binding
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processchromosome segregation

Inferred from electronic annotation. Source: Ensembl

kinetochore assembly

Inferred from electronic annotation. Source: InterPro

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcondensed chromosome kinetochore

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 639639Centromere protein T
PRO_0000417383

Regions

Region80 – 500421Flexible stalk domain

Secondary structure

.................... 639
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
F1NPG5 [UniParc].

Last modified May 16, 2012. Version 2.
Checksum: 9D314920A56ADC11

FASTA63970,670
        10         20         30         40         50         60 
MDGRVGLRAS RRAAPTPRVA VRSSPRHRAR VREQEPVVSA MSGSGLGKEN KAGSSTPLRH 

        70         80         90        100        110        120 
RPNAFSELDN ATPRVMLRKI IQNQPQVSPL ALQTVQLEET EDARPEPPSQ RTSSTVELQL 

       130        140        150        160        170        180 
PDLVPEDASV TTFRMTRKRK KLSISEFERA ADKRLPQNQA HSTLDSTLVR SLRMSVGSVM 

       190        200        210        220        230        240 
APDTVEKRGL LRRPQNHKAI DIAAFEGGVE QNMLQIKAQD YLVDLQTSSM TGTTTIRTDA 

       250        260        270        280        290        300 
EVVLNNTELF VEPQLGEQNL LAVEPQLSDS KTSAQRSNTS YPAHEKARLE GLVSRVSTDE 

       310        320        330        340        350        360 
RRTLRFSEKD LITDHEHVDG ITQKTPAKQG EEEQDHSQQN DPMEQFSESE EMAGTTEHHA 

       370        380        390        400        410        420 
DAEYSEHSEK KLSRKAVSQL TAAQDAGVEM EMTPSEGGVA EGTEHQDSPK AELQMAGSPG 

       430        440        450        460        470        480 
GHSPASYSLE KPGAKPLKEA VEQTGEIERG TITGVLDAAE EEATDDESDK EDHESEEISM 

       490        500        510        520        530        540 
KTPMFVHAAA YRPQPVLSPP HPVKSASPEL PPQPVRAKPV PKSSGAAQRK TREPEIASSL 

       550        560        570        580        590        600 
IKQIFSHYVK TPVTRDAYKI VEKCSERYFK QISSDLEAYS QHAGRKTVEM ADVELLMRRQ 

       610        620        630 
GLVTDKMPLH VLVERHLPLE YRKLLIPIAV SGNKVIPCK 

« Hide

References

« Hide 'large scale' references
[1]"A comprehensive collection of chicken cDNAs."
Boardman P.E., Sanz-Ezquerro J., Overton I.M., Burt D.W., Bosch E., Fong W.T., Tickle C., Brown W.R., Wilson S.A., Hubbard S.J.
Curr. Biol. 12:1965-1969(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: White Leghorn Hisex.
[2]"Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution."
Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A. expand/collapse author list , Kremitzki C., Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D., Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K., Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E., Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M., Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S., Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M., Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O., Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R., Wilson R.K.
Nature 432:695-716(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Red jungle fowl.
[3]"CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore."
Hori T., Amano M., Suzuki A., Backer C.B., Welburn J.P., Dong Y., McEwen B.F., Shang W.-H., Suzuki E., Okawa K., Cheeseman I.M., Fukagawa T.
Cell 135:1039-1052(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[4]"Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins."
Suzuki A., Hori T., Nishino T., Usukura J., Miyagi A., Morikawa K., Fukagawa T.
J. Cell Biol. 193:125-140(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH CENPW.
[5]"CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."
Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 54-162, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ452155 mRNA. No translation available.
BU128959 mRNA. No translation available.
BU260128 mRNA. No translation available.
BU381659 mRNA. No translation available.
BU382952 mRNA. No translation available.
BU433983 mRNA. No translation available.
CD734856 mRNA. No translation available.
AADN02051672 Genomic DNA. No translation available.
RefSeqNP_001263242.1. NM_001276313.1.
UniGeneGga.2263.
Gga.54685.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3B0CX-ray2.20T531-639[»]
3B0DX-ray2.20B/T531-639[»]
3VH5X-ray2.40T531-637[»]
3VH6X-ray3.35T531-637[»]
3VZAX-ray1.90E/F63-98[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActF1NPG5. 4 interactions.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID415654.
KEGGgga:415654.

Organism-specific databases

CTD80152.

Phylogenomic databases

KOK11512.

Family and domain databases

Gene3D1.10.20.10. 1 hit.
InterProIPR028255. CENP-T.
IPR009072. Histone-fold.
[Graphical view]
PANTHERPTHR14857. PTHR14857. 1 hit.
SUPFAMSSF47113. SSF47113. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCENPT_CHICK
AccessionPrimary (citable) accession number: F1NPG5
Entry history
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: May 16, 2012
Last modified: February 19, 2014
This is version 24 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references