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Protein

Centromere protein T

Gene

CENPT

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres (By similarity). Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.By similarity2 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW

GO - Biological processi

  1. cell division Source: UniProtKB-KW
  2. chromosome segregation Source: Ensembl
  3. kinetochore assembly Source: InterPro
  4. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_291865. Resolution of Sister Chromatid Cohesion.
REACT_297762. Separation of Sister Chromatids.
REACT_339698. Deposition of new CENPA-containing nucleosomes at the centromere.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein T
Short name:
CENP-T
Gene namesi
Name:CENPT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus.

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB-SubCell
  2. cytoplasm Source: Ensembl
  3. nuclear membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 639639Centromere protein TPRO_0000417383Add
BLAST

Expressioni

Gene expression databases

ExpressionAtlasiF1NPG5. baseline.

Interactioni

Subunit structurei

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU (By similarity). The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS (By similarity). Part of a centromere complex consisting of CENPA, CENPT and CENPW (By similarity). Part of a centromere complex consisting of histone H3, CENPT and CENPW. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPW. Interacts (via N-terminus) with the NDC80 complex. Binds DNA.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APITD1E1BSW73EBI-2132248,EBI-5487792
CENPWP0DJH64EBI-2132248,EBI-2132287

Protein-protein interaction databases

IntActiF1NPG5. 4 interactions.

Structurei

Secondary structure

1
639
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi66 – 705Combined sources
Helixi73 – 8311Combined sources
Helixi538 – 54912Combined sources
Helixi555 – 58228Combined sources
Beta strandi586 – 5883Combined sources
Helixi590 – 59910Combined sources
Beta strandi602 – 6043Combined sources
Beta strandi605 – 6073Combined sources
Helixi609 – 6168Combined sources
Helixi619 – 6257Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B0CX-ray2.20T531-639[»]
3B0DX-ray2.20B/T531-639[»]
3VH5X-ray2.40T531-637[»]
3VH6X-ray3.35T531-637[»]
3VZAX-ray1.90E/F63-98[»]
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 500421Flexible stalk domainAdd
BLAST

Domaini

The largest part of the sequence forms an elongated and flexible stalk structure that is connected to a C-terminal globular domain with a histone-type fold.1 Publication

Sequence similaritiesi

Belongs to the CENPT family.Curated

Phylogenomic databases

InParanoidiF1NPG5.
KOiK11512.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR028255. CENP-T.
IPR009072. Histone-fold.
[Graphical view]
PANTHERiPTHR14857. PTHR14857. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

F1NPG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGRVGLRAS RRAAPTPRVA VRSSPRHRAR VREQEPVVSA MSGSGLGKEN
60 70 80 90 100
KAGSSTPLRH RPNAFSELDN ATPRVMLRKI IQNQPQVSPL ALQTVQLEET
110 120 130 140 150
EDARPEPPSQ RTSSTVELQL PDLVPEDASV TTFRMTRKRK KLSISEFERA
160 170 180 190 200
ADKRLPQNQA HSTLDSTLVR SLRMSVGSVM APDTVEKRGL LRRPQNHKAI
210 220 230 240 250
DIAAFEGGVE QNMLQIKAQD YLVDLQTSSM TGTTTIRTDA EVVLNNTELF
260 270 280 290 300
VEPQLGEQNL LAVEPQLSDS KTSAQRSNTS YPAHEKARLE GLVSRVSTDE
310 320 330 340 350
RRTLRFSEKD LITDHEHVDG ITQKTPAKQG EEEQDHSQQN DPMEQFSESE
360 370 380 390 400
EMAGTTEHHA DAEYSEHSEK KLSRKAVSQL TAAQDAGVEM EMTPSEGGVA
410 420 430 440 450
EGTEHQDSPK AELQMAGSPG GHSPASYSLE KPGAKPLKEA VEQTGEIERG
460 470 480 490 500
TITGVLDAAE EEATDDESDK EDHESEEISM KTPMFVHAAA YRPQPVLSPP
510 520 530 540 550
HPVKSASPEL PPQPVRAKPV PKSSGAAQRK TREPEIASSL IKQIFSHYVK
560 570 580 590 600
TPVTRDAYKI VEKCSERYFK QISSDLEAYS QHAGRKTVEM ADVELLMRRQ
610 620 630
GLVTDKMPLH VLVERHLPLE YRKLLIPIAV SGNKVIPCK
Length:639
Mass (Da):70,670
Last modified:May 16, 2012 - v2
Checksum:i9D314920A56ADC11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ452155 mRNA. No translation available.
BU128959 mRNA. No translation available.
BU260128 mRNA. No translation available.
BU381659 mRNA. No translation available.
BU382952 mRNA. No translation available.
BU433983 mRNA. No translation available.
CD734856 mRNA. No translation available.
AADN02051672 Genomic DNA. No translation available.
RefSeqiNP_001263242.1. NM_001276313.1.
UniGeneiGga.2263.
Gga.54685.

Genome annotation databases

GeneIDi415654.
KEGGigga:415654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ452155 mRNA. No translation available.
BU128959 mRNA. No translation available.
BU260128 mRNA. No translation available.
BU381659 mRNA. No translation available.
BU382952 mRNA. No translation available.
BU433983 mRNA. No translation available.
CD734856 mRNA. No translation available.
AADN02051672 Genomic DNA. No translation available.
RefSeqiNP_001263242.1. NM_001276313.1.
UniGeneiGga.2263.
Gga.54685.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B0CX-ray2.20T531-639[»]
3B0DX-ray2.20B/T531-639[»]
3VH5X-ray2.40T531-637[»]
3VH6X-ray3.35T531-637[»]
3VZAX-ray1.90E/F63-98[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiF1NPG5. 4 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi415654.
KEGGigga:415654.

Organism-specific databases

CTDi80152.

Phylogenomic databases

InParanoidiF1NPG5.
KOiK11512.

Enzyme and pathway databases

ReactomeiREACT_291865. Resolution of Sister Chromatid Cohesion.
REACT_297762. Separation of Sister Chromatids.
REACT_339698. Deposition of new CENPA-containing nucleosomes at the centromere.

Gene expression databases

ExpressionAtlasiF1NPG5. baseline.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR028255. CENP-T.
IPR009072. Histone-fold.
[Graphical view]
PANTHERiPTHR14857. PTHR14857. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: White Leghorn Hisex.
  2. "Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution."
    Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A.
    , Kremitzki C., Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D., Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K., Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E., Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M., Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S., Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M., Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O., Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R., Wilson R.K.
    Nature 432:695-716(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Red jungle fowl.
  3. "CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore."
    Hori T., Amano M., Suzuki A., Backer C.B., Welburn J.P., Dong Y., McEwen B.F., Shang W.-H., Suzuki E., Okawa K., Cheeseman I.M., Fukagawa T.
    Cell 135:1039-1052(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  4. "Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins."
    Suzuki A., Hori T., Nishino T., Usukura J., Miyagi A., Morikawa K., Fukagawa T.
    J. Cell Biol. 193:125-140(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH CENPW.
  5. "CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."
    Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
    Cell 148:487-501(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 54-162, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCENPT_CHICK
AccessioniPrimary (citable) accession number: F1NPG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: May 16, 2012
Last modified: April 1, 2015
This is version 32 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.