SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

F1NPG5

- CENPT_CHICK

UniProt

F1NPG5 - CENPT_CHICK

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Centromere protein T
Gene
CENPT
Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation By similarity. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres By similarity. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.2 Publications

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. chromosome segregation Source: Ensembl
  2. kinetochore assembly Source: InterPro
  3. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198182. Resolution of Sister Chromatid Cohesion.
REACT_210486. Separation of Sister Chromatids.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein T
Short name:
CENP-T
Gene namesi
Name:CENPT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Unplaced

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Constitutively localizes to centromeres throughout the cell cycle, and to kinetochores during mitosis. Localizes to the inner kinetochore, and may connect it to the outer kinetochore via its N-terminus.3 Publications

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 639639Centromere protein T
PRO_0000417383Add
BLAST

Interactioni

Subunit structurei

Component of the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU By similarity. The CENPA-NAC complex interacts with the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS By similarity. Part of a centromere complex consisting of CENPA, CENPT and CENPW By similarity. Part of a centromere complex consisting of histone H3, CENPT and CENPW. Component of a heterotetrameric CENP-T-W-S-X complex composed of APITD1/CENPS, STRA13/CENPX, CENPT and CENPW. Interacts directly with CENPW. Interacts (via N-terminus) with the NDC80 complex. Binds DNA.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
APITD1E1BSW73EBI-2132248,EBI-5487792
CENPWP0DJH64EBI-2132248,EBI-2132287

Protein-protein interaction databases

IntActiF1NPG5. 4 interactions.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi66 – 705
Helixi73 – 8311
Helixi538 – 54912
Helixi555 – 58228
Beta strandi586 – 5883
Helixi590 – 59910
Beta strandi602 – 6043
Beta strandi605 – 6073
Helixi609 – 6168
Helixi619 – 6257

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3B0CX-ray2.20T531-639[»]
3B0DX-ray2.20B/T531-639[»]
3VH5X-ray2.40T531-637[»]
3VH6X-ray3.35T531-637[»]
3VZAX-ray1.90E/F63-98[»]

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni80 – 500421Flexible stalk domain
Add
BLAST

Domaini

The largest part of the sequence forms an elongated and flexible stalk structure that is connected to a C-terminal globular domain with a histone-type fold.1 Publication

Sequence similaritiesi

Belongs to the CENPT family.

Phylogenomic databases

KOiK11512.

Family and domain databases

Gene3Di1.10.20.10. 1 hit.
InterProiIPR028255. CENP-T.
IPR009072. Histone-fold.
[Graphical view]
PANTHERiPTHR14857. PTHR14857. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

F1NPG5-1 [UniParc]FASTAAdd to Basket

« Hide

MDGRVGLRAS RRAAPTPRVA VRSSPRHRAR VREQEPVVSA MSGSGLGKEN    50
KAGSSTPLRH RPNAFSELDN ATPRVMLRKI IQNQPQVSPL ALQTVQLEET 100
EDARPEPPSQ RTSSTVELQL PDLVPEDASV TTFRMTRKRK KLSISEFERA 150
ADKRLPQNQA HSTLDSTLVR SLRMSVGSVM APDTVEKRGL LRRPQNHKAI 200
DIAAFEGGVE QNMLQIKAQD YLVDLQTSSM TGTTTIRTDA EVVLNNTELF 250
VEPQLGEQNL LAVEPQLSDS KTSAQRSNTS YPAHEKARLE GLVSRVSTDE 300
RRTLRFSEKD LITDHEHVDG ITQKTPAKQG EEEQDHSQQN DPMEQFSESE 350
EMAGTTEHHA DAEYSEHSEK KLSRKAVSQL TAAQDAGVEM EMTPSEGGVA 400
EGTEHQDSPK AELQMAGSPG GHSPASYSLE KPGAKPLKEA VEQTGEIERG 450
TITGVLDAAE EEATDDESDK EDHESEEISM KTPMFVHAAA YRPQPVLSPP 500
HPVKSASPEL PPQPVRAKPV PKSSGAAQRK TREPEIASSL IKQIFSHYVK 550
TPVTRDAYKI VEKCSERYFK QISSDLEAYS QHAGRKTVEM ADVELLMRRQ 600
GLVTDKMPLH VLVERHLPLE YRKLLIPIAV SGNKVIPCK 639
Length:639
Mass (Da):70,670
Last modified:May 16, 2012 - v2
Checksum:i9D314920A56ADC11
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ452155 mRNA. No translation available.
BU128959 mRNA. No translation available.
BU260128 mRNA. No translation available.
BU381659 mRNA. No translation available.
BU382952 mRNA. No translation available.
BU433983 mRNA. No translation available.
CD734856 mRNA. No translation available.
AADN02051672 Genomic DNA. No translation available.
RefSeqiNP_001263242.1. NM_001276313.1.
UniGeneiGga.2263.
Gga.54685.

Genome annotation databases

GeneIDi415654.
KEGGigga:415654.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ452155 mRNA. No translation available.
BU128959 mRNA. No translation available.
BU260128 mRNA. No translation available.
BU381659 mRNA. No translation available.
BU382952 mRNA. No translation available.
BU433983 mRNA. No translation available.
CD734856 mRNA. No translation available.
AADN02051672 Genomic DNA. No translation available.
RefSeqi NP_001263242.1. NM_001276313.1.
UniGenei Gga.2263.
Gga.54685.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3B0C X-ray 2.20 T 531-639 [» ]
3B0D X-ray 2.20 B/T 531-639 [» ]
3VH5 X-ray 2.40 T 531-637 [» ]
3VH6 X-ray 3.35 T 531-637 [» ]
3VZA X-ray 1.90 E/F 63-98 [» ]
ModBasei Search...

Protein-protein interaction databases

IntActi F1NPG5. 4 interactions.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 415654.
KEGGi gga:415654.

Organism-specific databases

CTDi 80152.

Phylogenomic databases

KOi K11512.

Enzyme and pathway databases

Reactomei REACT_198182. Resolution of Sister Chromatid Cohesion.
REACT_210486. Separation of Sister Chromatids.

Family and domain databases

Gene3Di 1.10.20.10. 1 hit.
InterProi IPR028255. CENP-T.
IPR009072. Histone-fold.
[Graphical view ]
PANTHERi PTHR14857. PTHR14857. 1 hit.
SUPFAMi SSF47113. SSF47113. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: White Leghorn Hisex.
  2. "Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution."
    Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A.
    , Kremitzki C., Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D., Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K., Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E., Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M., Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S., Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M., Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O., Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R., Wilson R.K.
    Nature 432:695-716(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Red jungle fowl.
  3. "CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore."
    Hori T., Amano M., Suzuki A., Backer C.B., Welburn J.P., Dong Y., McEwen B.F., Shang W.-H., Suzuki E., Okawa K., Cheeseman I.M., Fukagawa T.
    Cell 135:1039-1052(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
  4. "Spindle microtubules generate tension-dependent changes in the distribution of inner kinetochore proteins."
    Suzuki A., Hori T., Nishino T., Usukura J., Miyagi A., Morikawa K., Fukagawa T.
    J. Cell Biol. 193:125-140(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, DOMAIN, INTERACTION WITH CENPW.
  5. "CENP-T-W-S-X forms a unique centromeric chromatin structure with a histone-like fold."
    Nishino T., Takeuchi K., Gascoigne K.E., Suzuki A., Hori T., Oyama T., Morikawa K., Cheeseman I.M., Fukagawa T.
    Cell 148:487-501(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 54-162, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiCENPT_CHICK
AccessioniPrimary (citable) accession number: F1NPG5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: May 16, 2012
Last modified: September 3, 2014
This is version 26 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi