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Protein

Centromere protein T

Gene

CENPT

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation (By similarity). The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres (By similarity). Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.By similarity2 Publications

Miscellaneous

Association with CENPA-containing complexes may be indirect and due to the proximity of centromeric nucleosomes containing histone H3 with those containing CENPA.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-binding
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-GGA-141444. Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal.
R-GGA-2467813. Separation of Sister Chromatids.
R-GGA-2500257. Resolution of Sister Chromatid Cohesion.
R-GGA-5663220. RHO GTPases Activate Formins.
R-GGA-606279. Deposition of new CENPA-containing nucleosomes at the centromere.
R-GGA-68877. Mitotic Prometaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Centromere protein T
Short name:
CENP-T
Gene namesi
Name:CENPT
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Chromosome 11

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004173831 – 639Centromere protein TAdd BLAST639

Proteomic databases

PaxDbiF1NPG5.

PTM databases

iPTMnetiF1NPG5.

Expressioni

Gene expression databases

BgeeiENSGALG00000028900.
ExpressionAtlasiF1NPG5. baseline.

Interactioni

Subunit structurei

Component of the CENPA-CAD complex, composed of CENPI, CENPK, CENPL, CENPO, CENPP, CENPQ, CENPR and CENPS. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex, at least composed of CENPA, CENPC, CENPH, CENPM, CENPN, CENPT and CENPU (By similarity). Identified in a centromeric complex containing histones H2A, H2B, H3 and H4, and at least CENPA, CENPB, CENPC, CENPT, CENPN, HJURP, SUPT16H, SSRP1 and RSF1 (By similarity). Interacts (via N-terminus) with the NDC80 complex (By similarity). Heterodimer with CENPW; this dimer coassembles with CENPS-CENPX heterodimers at centromeres to form the tetrameric CENP-T-W-S-X complex (PubMed:19070575, PubMed:21464230, PubMed:22304917).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

IntActiF1NPG5. 6 interactors.
STRINGi9031.ENSGALP00000002336.

Structurei

Secondary structure

1639
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi66 – 70Combined sources5
Helixi73 – 83Combined sources11
Helixi538 – 549Combined sources12
Helixi555 – 582Combined sources28
Beta strandi586 – 588Combined sources3
Helixi590 – 599Combined sources10
Beta strandi602 – 604Combined sources3
Beta strandi605 – 607Combined sources3
Helixi609 – 616Combined sources8
Helixi619 – 625Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3B0CX-ray2.20T531-639[»]
3B0DX-ray2.20B/T531-639[»]
3VH5X-ray2.40T531-637[»]
3VH6X-ray3.35T531-637[»]
3VZAX-ray1.90E/F63-98[»]
SMRiF1NPG5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni80 – 500Flexible stalk domainAdd BLAST421

Domaini

The largest part of the sequence forms an elongated and flexible stalk structure that is connected to a C-terminal globular domain with a histone-type fold.1 Publication

Sequence similaritiesi

Belongs to the CENPT family.Curated

Phylogenomic databases

eggNOGiENOG410IFJ0. Eukaryota.
ENOG4111ZWH. LUCA.
InParanoidiF1NPG5.
KOiK11512.
OrthoDBiEOG091G05OQ.

Family and domain databases

InterProiView protein in InterPro
IPR028255. CENP-T.
IPR035425. CENP-T/H4_C.
IPR032373. CENP-T_N.
IPR009072. Histone-fold.
PANTHERiPTHR10484:SF117. PTHR10484:SF117. 1 hit.
PfamiView protein in Pfam
PF15511. CENP-T_C. 1 hit.
PF16171. CENP-T_N. 1 hit.
SUPFAMiSSF47113. SSF47113. 1 hit.

Sequencei

Sequence statusi: Complete.

F1NPG5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGRVGLRAS RRAAPTPRVA VRSSPRHRAR VREQEPVVSA MSGSGLGKEN
60 70 80 90 100
KAGSSTPLRH RPNAFSELDN ATPRVMLRKI IQNQPQVSPL ALQTVQLEET
110 120 130 140 150
EDARPEPPSQ RTSSTVELQL PDLVPEDASV TTFRMTRKRK KLSISEFERA
160 170 180 190 200
ADKRLPQNQA HSTLDSTLVR SLRMSVGSVM APDTVEKRGL LRRPQNHKAI
210 220 230 240 250
DIAAFEGGVE QNMLQIKAQD YLVDLQTSSM TGTTTIRTDA EVVLNNTELF
260 270 280 290 300
VEPQLGEQNL LAVEPQLSDS KTSAQRSNTS YPAHEKARLE GLVSRVSTDE
310 320 330 340 350
RRTLRFSEKD LITDHEHVDG ITQKTPAKQG EEEQDHSQQN DPMEQFSESE
360 370 380 390 400
EMAGTTEHHA DAEYSEHSEK KLSRKAVSQL TAAQDAGVEM EMTPSEGGVA
410 420 430 440 450
EGTEHQDSPK AELQMAGSPG GHSPASYSLE KPGAKPLKEA VEQTGEIERG
460 470 480 490 500
TITGVLDAAE EEATDDESDK EDHESEEISM KTPMFVHAAA YRPQPVLSPP
510 520 530 540 550
HPVKSASPEL PPQPVRAKPV PKSSGAAQRK TREPEIASSL IKQIFSHYVK
560 570 580 590 600
TPVTRDAYKI VEKCSERYFK QISSDLEAYS QHAGRKTVEM ADVELLMRRQ
610 620 630
GLVTDKMPLH VLVERHLPLE YRKLLIPIAV SGNKVIPCK
Length:639
Mass (Da):70,670
Last modified:May 16, 2012 - v2
Checksum:i9D314920A56ADC11
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ452155 mRNA. No translation available.
BU128959 mRNA. No translation available.
BU260128 mRNA. No translation available.
BU381659 mRNA. No translation available.
BU382952 mRNA. No translation available.
BU433983 mRNA. No translation available.
CD734856 mRNA. No translation available.
AADN02051672 Genomic DNA. No translation available.
RefSeqiNP_001263242.1. NM_001276313.1.
UniGeneiGga.2263.
Gga.54685.

Genome annotation databases

EnsembliENSGALT00000043964; ENSGALP00000042737; ENSGALG00000028900.
GeneIDi415654.
KEGGigga:415654.

Similar proteinsi

Entry informationi

Entry nameiCENPT_CHICK
AccessioniPrimary (citable) accession number: F1NPG5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: May 16, 2012
Last modified: September 27, 2017
This is version 51 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families