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Protein

ATP-sensitive inward rectifier potassium channel 12

Gene

KCNJ12

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inward rectifying potassium channel that is activated by phosphatidylinositol 4,5-bisphosphate and that probably participates in controlling the resting membrane potential in electrically excitable cells. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. The inward rectification is probably due to the blockage of outward current by cytoplasmic polyamines and/or magnesium ions.2 Publications

GO - Molecular functioni

  • inward rectifier potassium channel activity Source: UniProtKB

GO - Biological processi

  • potassium ion import Source: GO_Central
  • potassium ion transport Source: UniProtKB
  • protein homotetramerization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-sensitive inward rectifier potassium channel 12
Alternative name(s):
Inward rectifier K(+) channel Kir2.2
Potassium channel, inwardly rectifying subfamily J member 12
Gene namesi
Name:KCNJ12
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 81CytoplasmicAdd BLAST81
Transmembranei82 – 106Helical; Name=M1Add BLAST25
Topological domaini107 – 123ExtracellularAdd BLAST17
Intramembranei124 – 1285
Intramembranei129 – 142Helical; Pore-forming; Name=H5Add BLAST14
Intramembranei143 – 151Pore-forming9
Topological domaini152 – 156Extracellular5
Transmembranei157 – 181Helical; Name=M2Add BLAST25
Topological domaini182 – 429CytoplasmicAdd BLAST248

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • intrinsic component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004225441 – 429ATP-sensitive inward rectifier potassium channel 12Add BLAST429

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi155Interchain1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiF1NHE9.

Expressioni

Gene expression databases

BgeeiENSGALG00000004721.

Interactioni

Subunit structurei

Homotetramer.2 Publications

Protein-protein interaction databases

DIPiDIP-59161N.
STRINGi9031.ENSGALP00000007506.

Structurei

Secondary structure

1429
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi54 – 57Combined sources4
Helixi62 – 68Combined sources7
Helixi70 – 76Combined sources7
Helixi79 – 108Combined sources30
Helixi110 – 112Combined sources3
Beta strandi113 – 115Combined sources3
Beta strandi123 – 125Combined sources3
Helixi130 – 141Combined sources12
Beta strandi147 – 151Combined sources5
Helixi156 – 184Combined sources29
Helixi187 – 193Combined sources7
Beta strandi194 – 196Combined sources3
Beta strandi200 – 205Combined sources6
Beta strandi208 – 217Combined sources10
Beta strandi219 – 221Combined sources3
Beta strandi223 – 237Combined sources15
Beta strandi243 – 250Combined sources8
Helixi255 – 257Combined sources3
Turni258 – 260Combined sources3
Beta strandi268 – 273Combined sources6
Turni279 – 282Combined sources4
Helixi285 – 289Combined sources5
Beta strandi294 – 303Combined sources10
Turni304 – 306Combined sources3
Beta strandi309 – 317Combined sources9
Helixi318 – 320Combined sources3
Beta strandi321 – 323Combined sources3
Beta strandi325 – 327Combined sources3
Beta strandi331 – 333Combined sources3
Beta strandi335 – 340Combined sources6
Helixi342 – 344Combined sources3
Beta strandi348 – 350Combined sources3
Helixi359 – 365Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JYCX-ray3.11A38-369[»]
3SPCX-ray2.45A38-369[»]
3SPGX-ray2.61A38-369[»]
3SPHX-ray3.00A38-369[»]
3SPIX-ray3.31A38-369[»]
3SPJX-ray3.31A38-369[»]
5KUKX-ray2.00A38-369[»]
5KUMX-ray2.80A38-369[»]
ProteinModelPortaliF1NHE9.
SMRiF1NHE9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi78 – 80Interaction with phosphatidylinositides3
Motifi143 – 148Selectivity filterCurated6
Motifi183 – 190Interaction with phosphatidylinositides8

Domaini

Phosphatidylinositol 4,5-bisphosphate binding to the cytoplasmic side of the channel triggers a conformation change leading to channel opening.1 Publication

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237325.
InParanoidiF1NHE9.
KOiK05005.
OrthoDBiEOG091G08HC.
PhylomeDBiF1NHE9.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003272. K_chnl_inward-rec_Kir2.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF14. PTHR11767:SF14. 1 hit.
PfamiPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PRINTSiPR01325. KIR22CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

F1NHE9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAGRVNPYS IVSSEEDGLR LTTMPGINGF GNGKIHTRRK CRNRFVKKNG
60 70 80 90 100
QCNVEFTNMD DKPQRYIADM FTTCVDIRWR YMLLLFSLAF LVSWLLFGLI
110 120 130 140 150
FWLIALIHGD LENPGGDDTF KPCVLQVNGF VAAFLFSIET QTTIGYGFRC
160 170 180 190 200
VTEECPLAVF MVVVQSIVGC IIDSFMIGAI MAKMARPKKR AQTLLFSHNA
210 220 230 240 250
VVAMRDGKLC LMWRVGNLRK SHIVEAHVRA QLIKPRITEE GEYIPLDQID
260 270 280 290 300
IDVGFDKGLD RIFLVSPITI LHEINEDSPL FGISRQDLET DDFEIVVILE
310 320 330 340 350
GMVEATAMTT QARSSYLASE ILWGHRFEPV LFEEKNQYKV DYSHFHKTYE
360 370 380 390 400
VPSTPRCSAK DLVENKFLLP STNSFCYENE LAFMSRDEDE EDDDSRGLDD
410 420
LSPDNRHEFD RLQATIALDQ RSYRRESEI
Length:429
Mass (Da):49,083
Last modified:May 3, 2011 - v1
Checksum:iE0AF2D60A03D06C1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AADN02023767 Genomic DNA. No translation available.
RefSeqiXP_004945226.1. XM_004945169.2.
XP_004945227.1. XM_004945170.2.
XP_015149865.1. XM_015294379.1.

Genome annotation databases

EnsembliENSGALT00000007518; ENSGALP00000007506; ENSGALG00000004721.
GeneIDi427662.
KEGGigga:427662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AADN02023767 Genomic DNA. No translation available.
RefSeqiXP_004945226.1. XM_004945169.2.
XP_004945227.1. XM_004945170.2.
XP_015149865.1. XM_015294379.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3JYCX-ray3.11A38-369[»]
3SPCX-ray2.45A38-369[»]
3SPGX-ray2.61A38-369[»]
3SPHX-ray3.00A38-369[»]
3SPIX-ray3.31A38-369[»]
3SPJX-ray3.31A38-369[»]
5KUKX-ray2.00A38-369[»]
5KUMX-ray2.80A38-369[»]
ProteinModelPortaliF1NHE9.
SMRiF1NHE9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-59161N.
STRINGi9031.ENSGALP00000007506.

Proteomic databases

PaxDbiF1NHE9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSGALT00000007518; ENSGALP00000007506; ENSGALG00000004721.
GeneIDi427662.
KEGGigga:427662.

Organism-specific databases

CTDi3768.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
HOGENOMiHOG000237325.
InParanoidiF1NHE9.
KOiK05005.
OrthoDBiEOG091G08HC.
PhylomeDBiF1NHE9.
TreeFamiTF313676.

Miscellaneous databases

PROiF1NHE9.

Gene expression databases

BgeeiENSGALG00000004721.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR003272. K_chnl_inward-rec_Kir2.2.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR013673. K_chnl_inward-rec_Kir_N.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF14. PTHR11767:SF14. 1 hit.
PfamiPF01007. IRK. 1 hit.
PF08466. IRK_N. 1 hit.
[Graphical view]
PRINTSiPR01325. KIR22CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCJ12_CHICK
AccessioniPrimary (citable) accession number: F1NHE9
Secondary accession number(s): D2YW45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2013
Last sequence update: May 3, 2011
Last modified: November 30, 2016
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.