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F1N9Y5

- KSYK_CHICK

UniProt

F1N9Y5 - KSYK_CHICK

Protein

Tyrosine-protein kinase SYK

Gene

SYK

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 29 (01 Oct 2014)
      Sequence version 2 (25 Jan 2012)
      Previous versions | rss
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    Functioni

    Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors.1 Publication

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Enzyme regulationi

    Autoinhibited. Intramolecular binding of the interdomains A and B (also called linker region) to parts of the catalytic domain keep the catalytic center in an inactive conformation. The phosphorylation of the interdomains or the binding of the SH2 domains with dually phosphorylated ITAM domains on transmembrane proteins disrupt those intramolecular interactions allowing the kinase domain to adopt an active conformation. The phosphorylation of SYK and of the ITAM domains which is responsible for SYK activation is essentially mediated by SRC subfamily kinases, like LYN, upon transmembrane receptors engagement. Downstream signaling adapters and intermediates may mediate positive and/or negative feedback regulation By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei380 – 3801ATPPROSITE-ProRule annotation
    Active sitei472 – 4721Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi355 – 3639ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB

    GO - Biological processi

    1. adaptive immune response Source: UniProtKB
    2. B cell receptor signaling pathway Source: UniProtKB
    3. blood vessel morphogenesis Source: UniProtKB
    4. cellular response to molecule of fungal origin Source: UniProtKB
    5. defense response to bacterium Source: UniProtKB
    6. innate immune response Source: UniProtKB
    7. integrin-mediated signaling pathway Source: UniProtKB
    8. intracellular signal transduction Source: InterPro
    9. leukocyte activation involved in immune response Source: UniProtKB
    10. leukocyte cell-cell adhesion Source: UniProtKB
    11. lymph vessel development Source: UniProtKB
    12. macrophage activation involved in immune response Source: UniProtKB
    13. neutrophil activation involved in immune response Source: UniProtKB
    14. neutrophil chemotaxis Source: UniProtKB
    15. peptidyl-tyrosine phosphorylation Source: GOC
    16. positive regulation of bone resorption Source: UniProtKB
    17. positive regulation of cell adhesion mediated by integrin Source: UniProtKB
    18. protein phosphorylation Source: UniProtKB
    19. regulation of arachidonic acid secretion Source: UniProtKB
    20. regulation of ERK1 and ERK2 cascade Source: UniProtKB
    21. regulation of neutrophil degranulation Source: UniProtKB
    22. regulation of phagocytosis Source: UniProtKB
    23. regulation of platelet activation Source: UniProtKB
    24. regulation of platelet aggregation Source: UniProtKB
    25. regulation of superoxide anion generation Source: UniProtKB
    26. serotonin secretion by platelet Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Biological processi

    Immunity, Innate immunity

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_189761. DAP12 signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase SYK (EC:2.7.10.2)
    Gene namesi
    Name:SYK
    ORF Names:RCJMB04_19o18
    OrganismiGallus gallus (Chicken)
    Taxonomic identifieri9031 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus
    ProteomesiUP000000539: Chromosome Z

    Subcellular locationi

    Cell membrane Curated. Cytoplasmcytosol Curated

    GO - Cellular componenti

    1. cytosol Source: UniProtKB-SubCell
    2. early phagosome Source: UniProtKB
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 613613Tyrosine-protein kinase SYKPRO_0000415332Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi9031.ENSGALP00000032170.

    Structurei

    3D structure databases

    ProteinModelPortaliF1N9Y5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 10693SH2 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini167 – 25892SH2 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini349 – 613265Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni107 – 16660Interdomain AAdd
    BLAST
    Regioni259 – 34890Interdomain BAdd
    BLAST

    Domaini

    The SH2 domains mediate the interaction of SYK with the phosphorylated ITAM domains of transmembrane proteins.By similarity

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 2 SH2 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH2 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115195.
    HOGENOMiHOG000113264.
    HOVERGENiHBG001540.
    InParanoidiQ5ZJ91.
    KOiK05855.
    OMAiKGYYQMK.
    OrthoDBiEOG7MWGWD.

    Family and domain databases

    Gene3Di1.10.930.10. 1 hit.
    3.30.505.10. 2 hits.
    InterProiIPR011009. Kinase-like_dom.
    IPR023420. Kinase_SYK/ZAP-70_inter-SH2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR012234. Tyr_kinase_non-rcpt_SYK/ZAP70.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000604. TyrPK_SYK. 1 hit.
    PRINTSiPR00401. SH2DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 2 hits.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55550. SSF55550. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    F1N9Y5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASNMANPAN HLPYFFGNIT REEAEEYLMQ GGMSDGLYLL RQSRNYLGGF    50
    ALSLAYGRKV HHYTIERELS GTYAIAGGKS HASPAELINY HSEEADGLIC 100
    LLRKSFNRPP GVEPKTGPFE DLKENLIREY VKQTWNLQGH ALEQAIISQK 150
    PQLEKLIATT AHEKMPWFHG RISREESEHR ILIGSRNNGK FLIRERDSNG 200
    SYALCLLNDG KVLHYRIDRD KTGKLSIPDG KRFDTLWQLV EHYSYKPDGL 250
    LRVLSIPCPR HGSESDNVVF DTRPLPGTPS KLQTPIGAPS DDQTPFNPYV 300
    LQRARGLIGA EKGDQREALP MDTEVYESPY ADPDEIKPKN VTLDRKLLTL 350
    EEGELGSGNF GTVKKGFYKM KKGAKPVAVK ILKNESNDPA IKDELLREAN 400
    VMQQLDNPYI VRMIGICEAE AWMLVMEMAE LGPLNKFLQK NRHVTEKNIT 450
    ELVHQVSMGM KYLEENNFVH RDLAARNVLL VTQHYAKISD FGLSKALSAD 500
    ENYYKAQSHG KWPVKWYAPE CMNFYKFSSK SDVWSFGVLM WEAFSYGQKP 550
    YKGMKGGEVA QMIERGERME CPEACPVEVY DLMKLCWTYN VDDRPGFVAV 600
    ELRLRNYYYD ISH 613
    Length:613
    Mass (Da):69,901
    Last modified:January 25, 2012 - v2
    Checksum:i32783B7D0C8EBC82
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti188 – 1881N → D in CAG32202. (PubMed:15642098)Curated
    Sequence conflicti398 – 3981E → G in CAG32202. (PubMed:15642098)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720543 mRNA. Translation: CAG32202.1.
    AADN02069860 Genomic DNA. No translation available.
    AADN02069861 Genomic DNA. No translation available.
    AADN02069862 Genomic DNA. No translation available.
    AADN02069863 Genomic DNA. No translation available.
    AADN02069864 Genomic DNA. No translation available.
    AADN02069865 Genomic DNA. No translation available.
    AADN02069866 Genomic DNA. No translation available.
    AADN02069867 Genomic DNA. No translation available.
    AADN02069868 Genomic DNA. No translation available.
    AADN02069869 Genomic DNA. No translation available.
    RefSeqiNP_001026601.1. NM_001031430.1.
    UniGeneiGga.22529.

    Genome annotation databases

    EnsembliENSGALT00000032807; ENSGALP00000032170; ENSGALG00000015216.
    GeneIDi427272.
    KEGGigga:427272.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ720543 mRNA. Translation: CAG32202.1 .
    AADN02069860 Genomic DNA. No translation available.
    AADN02069861 Genomic DNA. No translation available.
    AADN02069862 Genomic DNA. No translation available.
    AADN02069863 Genomic DNA. No translation available.
    AADN02069864 Genomic DNA. No translation available.
    AADN02069865 Genomic DNA. No translation available.
    AADN02069866 Genomic DNA. No translation available.
    AADN02069867 Genomic DNA. No translation available.
    AADN02069868 Genomic DNA. No translation available.
    AADN02069869 Genomic DNA. No translation available.
    RefSeqi NP_001026601.1. NM_001031430.1.
    UniGenei Gga.22529.

    3D structure databases

    ProteinModelPortali F1N9Y5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9031.ENSGALP00000032170.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSGALT00000032807 ; ENSGALP00000032170 ; ENSGALG00000015216 .
    GeneIDi 427272.
    KEGGi gga:427272.

    Organism-specific databases

    CTDi 6850.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115195.
    HOGENOMi HOG000113264.
    HOVERGENi HBG001540.
    InParanoidi Q5ZJ91.
    KOi K05855.
    OMAi KGYYQMK.
    OrthoDBi EOG7MWGWD.

    Enzyme and pathway databases

    Reactomei REACT_189761. DAP12 signaling.

    Miscellaneous databases

    NextBioi 20828556.
    PROi F1N9Y5.

    Family and domain databases

    Gene3Di 1.10.930.10. 1 hit.
    3.30.505.10. 2 hits.
    InterProi IPR011009. Kinase-like_dom.
    IPR023420. Kinase_SYK/ZAP-70_inter-SH2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR012234. Tyr_kinase_non-rcpt_SYK/ZAP70.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000604. TyrPK_SYK. 1 hit.
    PRINTSi PR00401. SH2DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 2 hits.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55550. SSF55550. 2 hits.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: CB.
      Tissue: Bursa of Fabricius.
    2. "Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution."
      Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A.
      , Kremitzki C., Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D., Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K., Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E., Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M., Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S., Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M., Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O., Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R., Wilson R.K.
      Nature 432:695-716(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Red jungle fowl.
    3. "BLNK: a central linker protein in B cell activation."
      Fu C., Turck C.W., Kurosaki T., Chan A.C.
      Immunity 9:93-103(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN B-CELL RECEPTOR SIGNALING PATHWAY, FUNCTION IN PHOSPHORYLATION OF BLNK.

    Entry informationi

    Entry nameiKSYK_CHICK
    AccessioniPrimary (citable) accession number: F1N9Y5
    Secondary accession number(s): Q5ZJ91
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: January 25, 2012
    Last modified: October 1, 2014
    This is version 29 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3