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F1N9Y5

- KSYK_CHICK

UniProt

F1N9Y5 - KSYK_CHICK

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Protein

Tyrosine-protein kinase SYK

Gene

SYK

Organism
Gallus gallus (Chicken)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development. Assembles into signaling complexes with activated receptors at the plasma membrane via interaction between its SH2 domains and the receptor tyrosine-phosphorylated ITAM domains. The association with the receptor can also be indirect and mediated by adapter proteins containing ITAM or partial hemITAM domains. The phosphorylation of the ITAM domains is generally mediated by SRC subfamily kinases upon engagement of the receptor. More rarely signal transduction via SYK could be ITAM-independent. Direct downstream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cell receptor (BCR) signaling, it is necessary for the maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Enzyme regulationi

Autoinhibited. Intramolecular binding of the interdomains A and B (also called linker region) to parts of the catalytic domain keep the catalytic center in an inactive conformation. The phosphorylation of the interdomains or the binding of the SH2 domains with dually phosphorylated ITAM domains on transmembrane proteins disrupt those intramolecular interactions allowing the kinase domain to adopt an active conformation. The phosphorylation of SYK and of the ITAM domains which is responsible for SYK activation is essentially mediated by SRC subfamily kinases, like LYN, upon transmembrane receptors engagement. Downstream signaling adapters and intermediates may mediate positive and/or negative feedback regulation (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei380 – 3801ATPPROSITE-ProRule annotation
Active sitei472 – 4721Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi355 – 3639ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB

GO - Biological processi

  1. adaptive immune response Source: UniProtKB
  2. B cell receptor signaling pathway Source: UniProtKB
  3. blood vessel morphogenesis Source: UniProtKB
  4. cellular response to molecule of fungal origin Source: UniProtKB
  5. defense response to bacterium Source: UniProtKB
  6. innate immune response Source: UniProtKB
  7. integrin-mediated signaling pathway Source: UniProtKB
  8. intracellular signal transduction Source: InterPro
  9. leukocyte activation involved in immune response Source: UniProtKB
  10. leukocyte cell-cell adhesion Source: UniProtKB
  11. lymph vessel development Source: UniProtKB
  12. macrophage activation involved in immune response Source: UniProtKB
  13. neutrophil activation involved in immune response Source: UniProtKB
  14. neutrophil chemotaxis Source: UniProtKB
  15. peptidyl-tyrosine phosphorylation Source: GOC
  16. positive regulation of bone resorption Source: UniProtKB
  17. positive regulation of cell adhesion mediated by integrin Source: UniProtKB
  18. protein phosphorylation Source: UniProtKB
  19. regulation of arachidonic acid secretion Source: UniProtKB
  20. regulation of ERK1 and ERK2 cascade Source: UniProtKB
  21. regulation of neutrophil degranulation Source: UniProtKB
  22. regulation of phagocytosis Source: UniProtKB
  23. regulation of platelet activation Source: UniProtKB
  24. regulation of platelet aggregation Source: UniProtKB
  25. regulation of superoxide anion generation Source: UniProtKB
  26. serotonin secretion by platelet Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Immunity, Innate immunity

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_189761. DAP12 signaling.
REACT_194994. Role of phospholipids in phagocytosis.
REACT_197606. Regulation of actin dynamics for phagocytic cup formation.
REACT_230602. GPVI-mediated activation cascade.
REACT_236561. Interleukin-2 signaling.
REACT_257604. Integrin alphaIIb beta3 signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase SYK (EC:2.7.10.2)
Gene namesi
Name:SYK
ORF Names:RCJMB04_19o18
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539: Chromosome Z

Subcellular locationi

Cell membrane Curated. Cytoplasmcytosol Curated

GO - Cellular componenti

  1. early phagosome Source: UniProtKB
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 613613Tyrosine-protein kinase SYKPRO_0000415332Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi9031.ENSGALP00000032170.

Structurei

3D structure databases

ProteinModelPortaliF1N9Y5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 10693SH2 1PROSITE-ProRule annotationAdd
BLAST
Domaini167 – 25892SH2 2PROSITE-ProRule annotationAdd
BLAST
Domaini349 – 613265Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni107 – 16660Interdomain AAdd
BLAST
Regioni259 – 34890Interdomain BAdd
BLAST

Domaini

The SH2 domains mediate the interaction of SYK with the phosphorylated ITAM domains of transmembrane proteins.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 2 SH2 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118799.
HOGENOMiHOG000113264.
HOVERGENiHBG001540.
InParanoidiF1N9Y5.
KOiK05855.
OMAiKGYYQMK.
OrthoDBiEOG7MWGWD.

Family and domain databases

Gene3Di1.10.930.10. 1 hit.
3.30.505.10. 2 hits.
InterProiIPR011009. Kinase-like_dom.
IPR023420. Kinase_SYK/ZAP-70_inter-SH2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR012234. Tyr_kinase_non-rcpt_SYK/ZAP70.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view]
PIRSFiPIRSF000604. TyrPK_SYK. 1 hit.
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

F1N9Y5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASNMANPAN HLPYFFGNIT REEAEEYLMQ GGMSDGLYLL RQSRNYLGGF
60 70 80 90 100
ALSLAYGRKV HHYTIERELS GTYAIAGGKS HASPAELINY HSEEADGLIC
110 120 130 140 150
LLRKSFNRPP GVEPKTGPFE DLKENLIREY VKQTWNLQGH ALEQAIISQK
160 170 180 190 200
PQLEKLIATT AHEKMPWFHG RISREESEHR ILIGSRNNGK FLIRERDSNG
210 220 230 240 250
SYALCLLNDG KVLHYRIDRD KTGKLSIPDG KRFDTLWQLV EHYSYKPDGL
260 270 280 290 300
LRVLSIPCPR HGSESDNVVF DTRPLPGTPS KLQTPIGAPS DDQTPFNPYV
310 320 330 340 350
LQRARGLIGA EKGDQREALP MDTEVYESPY ADPDEIKPKN VTLDRKLLTL
360 370 380 390 400
EEGELGSGNF GTVKKGFYKM KKGAKPVAVK ILKNESNDPA IKDELLREAN
410 420 430 440 450
VMQQLDNPYI VRMIGICEAE AWMLVMEMAE LGPLNKFLQK NRHVTEKNIT
460 470 480 490 500
ELVHQVSMGM KYLEENNFVH RDLAARNVLL VTQHYAKISD FGLSKALSAD
510 520 530 540 550
ENYYKAQSHG KWPVKWYAPE CMNFYKFSSK SDVWSFGVLM WEAFSYGQKP
560 570 580 590 600
YKGMKGGEVA QMIERGERME CPEACPVEVY DLMKLCWTYN VDDRPGFVAV
610
ELRLRNYYYD ISH
Length:613
Mass (Da):69,901
Last modified:January 25, 2012 - v2
Checksum:i32783B7D0C8EBC82
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881N → D in CAG32202. (PubMed:15642098)Curated
Sequence conflicti398 – 3981E → G in CAG32202. (PubMed:15642098)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720543 mRNA. Translation: CAG32202.1.
AADN02069860 Genomic DNA. No translation available.
AADN02069861 Genomic DNA. No translation available.
AADN02069862 Genomic DNA. No translation available.
AADN02069863 Genomic DNA. No translation available.
AADN02069864 Genomic DNA. No translation available.
AADN02069865 Genomic DNA. No translation available.
AADN02069866 Genomic DNA. No translation available.
AADN02069867 Genomic DNA. No translation available.
AADN02069868 Genomic DNA. No translation available.
AADN02069869 Genomic DNA. No translation available.
RefSeqiNP_001026601.1. NM_001031430.1.
UniGeneiGga.22529.

Genome annotation databases

EnsembliENSGALT00000032807; ENSGALP00000032170; ENSGALG00000015216.
GeneIDi427272.
KEGGigga:427272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ720543 mRNA. Translation: CAG32202.1 .
AADN02069860 Genomic DNA. No translation available.
AADN02069861 Genomic DNA. No translation available.
AADN02069862 Genomic DNA. No translation available.
AADN02069863 Genomic DNA. No translation available.
AADN02069864 Genomic DNA. No translation available.
AADN02069865 Genomic DNA. No translation available.
AADN02069866 Genomic DNA. No translation available.
AADN02069867 Genomic DNA. No translation available.
AADN02069868 Genomic DNA. No translation available.
AADN02069869 Genomic DNA. No translation available.
RefSeqi NP_001026601.1. NM_001031430.1.
UniGenei Gga.22529.

3D structure databases

ProteinModelPortali F1N9Y5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9031.ENSGALP00000032170.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSGALT00000032807 ; ENSGALP00000032170 ; ENSGALG00000015216 .
GeneIDi 427272.
KEGGi gga:427272.

Organism-specific databases

CTDi 6850.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000118799.
HOGENOMi HOG000113264.
HOVERGENi HBG001540.
InParanoidi F1N9Y5.
KOi K05855.
OMAi KGYYQMK.
OrthoDBi EOG7MWGWD.

Enzyme and pathway databases

Reactomei REACT_189761. DAP12 signaling.
REACT_194994. Role of phospholipids in phagocytosis.
REACT_197606. Regulation of actin dynamics for phagocytic cup formation.
REACT_230602. GPVI-mediated activation cascade.
REACT_236561. Interleukin-2 signaling.
REACT_257604. Integrin alphaIIb beta3 signaling.

Miscellaneous databases

NextBioi 20828556.
PROi F1N9Y5.

Family and domain databases

Gene3Di 1.10.930.10. 1 hit.
3.30.505.10. 2 hits.
InterProi IPR011009. Kinase-like_dom.
IPR023420. Kinase_SYK/ZAP-70_inter-SH2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR012234. Tyr_kinase_non-rcpt_SYK/ZAP70.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 2 hits.
[Graphical view ]
PIRSFi PIRSF000604. TyrPK_SYK. 1 hit.
PRINTSi PR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF55550. SSF55550. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: CB.
    Tissue: Bursa of Fabricius.
  2. "Sequence and comparative analysis of the chicken genome provide unique perspectives on vertebrate evolution."
    Hillier L.W., Miller W., Birney E., Warren W., Hardison R.C., Ponting C.P., Bork P., Burt D.W., Groenen M.A.M., Delany M.E., Dodgson J.B., Chinwalla A.T., Cliften P.F., Clifton S.W., Delehaunty K.D., Fronick C., Fulton R.S., Graves T.A.
    , Kremitzki C., Layman D., Magrini V., McPherson J.D., Miner T.L., Minx P., Nash W.E., Nhan M.N., Nelson J.O., Oddy L.G., Pohl C.S., Randall-Maher J., Smith S.M., Wallis J.W., Yang S.-P., Romanov M.N., Rondelli C.M., Paton B., Smith J., Morrice D., Daniels L., Tempest H.G., Robertson L., Masabanda J.S., Griffin D.K., Vignal A., Fillon V., Jacobbson L., Kerje S., Andersson L., Crooijmans R.P., Aerts J., van der Poel J.J., Ellegren H., Caldwell R.B., Hubbard S.J., Grafham D.V., Kierzek A.M., McLaren S.R., Overton I.M., Arakawa H., Beattie K.J., Bezzubov Y., Boardman P.E., Bonfield J.K., Croning M.D.R., Davies R.M., Francis M.D., Humphray S.J., Scott C.E., Taylor R.G., Tickle C., Brown W.R.A., Rogers J., Buerstedde J.-M., Wilson S.A., Stubbs L., Ovcharenko I., Gordon L., Lucas S., Miller M.M., Inoko H., Shiina T., Kaufman J., Salomonsen J., Skjoedt K., Wong G.K.-S., Wang J., Liu B., Wang J., Yu J., Yang H., Nefedov M., Koriabine M., Dejong P.J., Goodstadt L., Webber C., Dickens N.J., Letunic I., Suyama M., Torrents D., von Mering C., Zdobnov E.M., Makova K., Nekrutenko A., Elnitski L., Eswara P., King D.C., Yang S.-P., Tyekucheva S., Radakrishnan A., Harris R.S., Chiaromonte F., Taylor J., He J., Rijnkels M., Griffiths-Jones S., Ureta-Vidal A., Hoffman M.M., Severin J., Searle S.M.J., Law A.S., Speed D., Waddington D., Cheng Z., Tuzun E., Eichler E., Bao Z., Flicek P., Shteynberg D.D., Brent M.R., Bye J.M., Huckle E.J., Chatterji S., Dewey C., Pachter L., Kouranov A., Mourelatos Z., Hatzigeorgiou A.G., Paterson A.H., Ivarie R., Brandstrom M., Axelsson E., Backstrom N., Berlin S., Webster M.T., Pourquie O., Reymond A., Ucla C., Antonarakis S.E., Long M., Emerson J.J., Betran E., Dupanloup I., Kaessmann H., Hinrichs A.S., Bejerano G., Furey T.S., Harte R.A., Raney B., Siepel A., Kent W.J., Haussler D., Eyras E., Castelo R., Abril J.F., Castellano S., Camara F., Parra G., Guigo R., Bourque G., Tesler G., Pevzner P.A., Smit A., Fulton L.A., Mardis E.R., Wilson R.K.
    Nature 432:695-716(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Red jungle fowl.
  3. "BLNK: a central linker protein in B cell activation."
    Fu C., Turck C.W., Kurosaki T., Chan A.C.
    Immunity 9:93-103(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN B-CELL RECEPTOR SIGNALING PATHWAY, FUNCTION IN PHOSPHORYLATION OF BLNK.

Entry informationi

Entry nameiKSYK_CHICK
AccessioniPrimary (citable) accession number: F1N9Y5
Secondary accession number(s): Q5ZJ91
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 25, 2012
Last modified: November 26, 2014
This is version 31 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3