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Protein

2'-5'-oligoadenylate synthase 2

Gene

OAS2

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. Activated by detection of double stranded RNA (dsRNA): polymerizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNASEL) leading to its dimerization and subsequent activation. Activation of RNASEL leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNASEL-dependent pathway or an alternative antiviral pathway independent of RNASEL. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation (By similarity). May act as a negative regulator of lactation, stopping lactation in virally infected mammary gland lobules, thereby preventing transmission of viruses to neonates (By similarity). Non-infected lobules would not be affected, allowing efficient pup feeding during infection (By similarity).By similarity

Catalytic activityi

3 ATP = pppA2'p5'A2'p5'A + 2 diphosphate.By similarity

Cofactori

Mg2+By similarity

Enzyme regulationi

Produced as a latent enzyme which is activated by double stranded RNA (dsRNA) generated during the course of viral infection. The dsRNA activator must be at least 15 nucleotides long, and no modification of the 2'-hydroxyl group is tolerated. ssRNA or dsDNA do not act as activators. Strongly inhibited by copper, iron and zinc ions. Partially inhibited by cobalt and nickel ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei397ATPBy similarity1
Metal bindingi409Magnesium; catalyticSequence analysis1
Metal bindingi480Magnesium; catalyticSequence analysis1
Binding sitei544ATPBy similarity1
Binding sitei547ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, RNA-binding, Transferase
Biological processAntiviral defense, Immunity, Innate immunity
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
2'-5'-oligoadenylate synthase 2 (EC:2.7.7.84)
Short name:
(2-5')oligo(A) synthase 2
Short name:
2-5A synthase 2
Gene namesi
Name:OAS2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 17

Organism-specific databases

VGNCiVGNC:32391 OAS2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00004186272 – 7142'-5'-oligoadenylate synthase 2Add BLAST713

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1

Post-translational modificationi

Myristoylation is not essential for its activity.By similarity
Glycosylated. Glycosylation is essential for its activity.By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Myristate

Proteomic databases

PaxDbiF1N3B8
PRIDEiF1N3B8

Expressioni

Gene expression databases

BgeeiENSBTAG00000014628

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019477

Structurei

3D structure databases

SMRiF1N3B8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni11 – 336OAS domain 1Add BLAST326
Regioni344 – 683OAS domain 2Add BLAST340

Sequence similaritiesi

Belongs to the 2-5A synthase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0001 Eukaryota
COG5272 LUCA
GeneTreeiENSGT00510000046406
HOGENOMiHOG000013200
HOVERGENiHBG007855
InParanoidiF1N3B8
KOiK14216
OMAiKSYTSQK
OrthoDBiEOG091G0160
TreeFamiTF329749

Family and domain databases

Gene3Di1.10.1410.20, 2 hits
InterProiView protein in InterPro
IPR006117 2-5-oligoadenylate_synth_CS
IPR006116 2-5-oligoadenylate_synth_N
IPR018952 2-5-oligoAdlate_synth_1_dom2/C
IPR038121 2-5-oligoAdlate_synth_2_sf
IPR026774 2-5A_synthase
IPR002934 Polymerase_NTP_transf_dom
PANTHERiPTHR11258 PTHR11258, 1 hit
PfamiView protein in Pfam
PF01909 NTP_transf_2, 1 hit
PF10421 OAS1_C, 2 hits
PROSITEiView protein in PROSITE
PS00833 25A_SYNTH_2, 2 hits
PS50152 25A_SYNTH_3, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

F1N3B8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRESHLYE KPSEKLEEFI QNHLRPSEDC QKDIDQSVDT ICEVLQEPCP
60 70 80 90 100
SLTVTGVAKG GSYGRRTVLR GNSDGILVVF FGDLEQFQDQ EKRQYELLSK
110 120 130 140 150
IWAQMKHCES TWKLAAKMEL QNTNRSSRVT IQLSTKQQSI TFNVLPAFNA
160 170 180 190 200
LGLSEKSSLW SYRELKRSLD MVKARPGEFS VCFTELQEKF FSNYPSKLKD
210 220 230 240 250
LILLVKHWFQ KCQEKLINSS LLPPYALELL TVYAWEQGCG AEDFDMAEGV
260 270 280 290 300
RTVLRLIEKQ EQLCVYWTVN YNFGDEIVRN ILLSQLQAPR PVILDPTDPT
310 320 330 340 350
NNVSMDNTCW LQLKHEAQNW LRSLRQNESP GPSWNVLPAS LYITPGHLLD
360 370 380 390 400
KFVKDFLQPN QTFQDQIKKA LKIICSFLEE NCFRHSTTKI QVIQGGSTVK
410 420 430 440 450
GTALKTGSDA SLVVFANSLK SYTSPKNERY NIIKEIHEQL EACRQEKDFE
460 470 480 490 500
VKFEISKWKP PWVLSFTLKS KVLNESVDFD VLPAFNALGE LKSGSTPSPR
510 520 530 540 550
TYTELIHLYK PSDVFLEGEF SACFTKLQRN FVRSLPLKLK DLIRLLKHWY
560 570 580 590 600
CGCEKKLKQK GSLPPKYALE LLSIYAWEKG SGAQDFDMAE GFRTVLELVI
610 620 630 640 650
QYQHLCVFWT VNYSFDDEIL RNFLLGQIRR TRPVILDPAD PTGDVGGGHR
660 670 680 690 700
WCWHLLAKEA TEWLSSLCFK DKSGCPIQPW NVPKKRVQTP GSCGAGIYSM
710
VNEMHLLRSH RFLD
Length:714
Mass (Da):81,967
Last modified:May 3, 2011 - v1
Checksum:i6962A98CC6D437BA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161S → T in AAT44896 (PubMed:17024523).Curated1
Sequence conflicti219S → P in AAT44896 (PubMed:17024523).Curated1
Sequence conflicti277I → T in AAT44896 (PubMed:17024523).Curated1
Sequence conflicti522A → V in AAT44896 (PubMed:17024523).Curated1
Sequence conflicti551C → K in AAT44896 (PubMed:17024523).Curated1
Sequence conflicti573S → T in AAT44896 (PubMed:17024523).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY599197 mRNA Translation: AAT44896.1
DAAA02045423 Genomic DNA No translation available.
DAAA02045424 Genomic DNA No translation available.
DAAA02045425 Genomic DNA No translation available.
RefSeqiNP_001019728.1, NM_001024557.1
UniGeneiBt.41610

Genome annotation databases

EnsembliENSBTAT00000019477; ENSBTAP00000019477; ENSBTAG00000014628
GeneIDi529660
KEGGibta:529660

Similar proteinsi

Entry informationi

Entry nameiOAS2_BOVIN
AccessioniPrimary (citable) accession number: F1N3B8
Secondary accession number(s): Q53AV7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: May 3, 2011
Last modified: April 25, 2018
This is version 46 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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