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Protein

Arachidonate 12-lipoxygenase, 12S-type

Gene

Alox12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-heme iron-containing dioxygenase that catalyzes the stereo-specific peroxidation of free and esterified polyunsaturated fatty acids generating a spectrum of bioactive lipid mediators. Mainly converts arachidonic acid to (12S)-hydroperoxyeicosatetraenoic acid/(12S)-HPETE but can also metabolize linoleic acid. Has a dual activity since it also converts leukotriene A4/LTA4 into both the bioactive lipoxin A4/LXA4 and lipoxin B4/LXB4. Through the production of specific bioactive lipids like (12S)-HPETE it regulates different biological processes including platelet activation. It also probably positively regulates angiogenesis through regulation of the expression of the vascular endothelial growth factor. Plays a role in apoptotic process, promoting the survival of vascular smooth muscle cells for instance. May also play a role in the control of cell migration and proliferation.2 Publications

Catalytic activityi

Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.1 Publication
(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (5S,6R,15S)-trihydroxy-(7E,9E,11Z,13E)-eicosatetraenoate.1 Publication
(7E,9E,11Z,14Z)-(5S,6S)-5,6-epoxyicosa-7,9,11,14-tetraenoate + H2O = (5S,14R,15S)-trihydroxy-(6E,8Z,10E,12E)-eicosatetraenoate.1 Publication

Cofactori

Fe cationPROSITE-ProRule annotationNote: Binds 1 Fe cation per subunit.PROSITE-ProRule annotation

Enzyme regulationi

Activated by EGF.By similarity

Pathwayi: hydroperoxy eicosatetraenoic acid biosynthesis

This protein is involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis, which is part of Lipid metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway hydroperoxy eicosatetraenoic acid biosynthesis and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi360Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi365Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi540Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi544Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi663Iron; via carboxylate; catalyticPROSITE-ProRule annotation1

GO - Molecular functioni

  • arachidonate 12-lipoxygenase activity Source: RGD
  • hydrolase activity Source: UniProtKB-KW
  • iron ion binding Source: InterPro

GO - Biological processi

  • aging Source: RGD
  • arachidonic acid metabolic process Source: UniProtKB
  • cellular response to lipid Source: RGD
  • establishment of skin barrier Source: UniProtKB
  • hepoxilin biosynthetic process Source: UniProtKB
  • leukotriene A4 metabolic process Source: UniProtKB
  • linoleic acid metabolic process Source: UniProtKB
  • lipoxin A4 biosynthetic process Source: UniProtKB
  • lipoxin B4 biosynthetic process Source: UniProtKB
  • lipoxygenase pathway Source: UniProtKB
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of muscle cell apoptotic process Source: UniProtKB
  • negative regulation of platelet aggregation Source: UniProtKB
  • positive regulation of apoptotic process Source: RGD
  • positive regulation of blood vessel diameter Source: RGD
  • positive regulation of cell death Source: RGD
  • positive regulation of cell proliferation Source: RGD
  • positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
  • positive regulation of endothelial cell differentiation Source: RGD
  • positive regulation of endothelial cell migration Source: RGD
  • positive regulation of gene expression Source: RGD
  • positive regulation of mitochondrial depolarization Source: RGD
  • positive regulation of smooth muscle cell proliferation Source: RGD

Keywordsi

Molecular functionDioxygenase, Hydrolase, Oxidoreductase
Biological processFatty acid metabolism, Lipid metabolism
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-2142696 Synthesis of Hepoxilins (HX) and Trioxilins (TrX)
R-RNO-2142700 Synthesis of Lipoxins (LX)
R-RNO-2142712 Synthesis of 12-eicosatetraenoic acid derivatives
R-RNO-9018677 Biosynthesis of DHA-derived SPMs
R-RNO-9025106 Biosynthesis of DPAn-6 SPMs
R-RNO-9026290 Biosynthesis of DPAn-3-derived maresins
UniPathwayiUPA00881

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 12-lipoxygenase, 12S-type (EC:1.13.11.31)
Short name:
12S-LOX
Short name:
12S-lipoxygenase
Alternative name(s):
Lipoxin synthase 12-LO (EC:3.3.2.-)
Platelet-type lipoxygenase 12
Gene namesi
Name:Alox12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi1311159 Alox12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004238521 – 663Arachidonate 12-lipoxygenase, 12S-typeAdd BLAST663

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei246PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiF1LQ70
PRIDEiF1LQ70

PTM databases

iPTMnetiF1LQ70
PhosphoSitePlusiF1LQ70

Expressioni

Gene expression databases

BgeeiENSRNOG00000027037
ExpressionAtlasiF1LQ70 baseline and differential
GenevisibleiF1LQ70 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034009

Structurei

3D structure databases

SMRiF1LQ70
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 114PLATPROSITE-ProRule annotationAdd BLAST113
Domaini114 – 663LipoxygenasePROSITE-ProRule annotationAdd BLAST550

Sequence similaritiesi

Belongs to the lipoxygenase family.Curated

Phylogenomic databases

eggNOGiENOG410IF0U Eukaryota
ENOG410YN4N LUCA
GeneTreeiENSGT00550000074415
InParanoidiF1LQ70
OMAiMTITWHL
OrthoDBiEOG091G04A4
TreeFamiTF105320

Family and domain databases

InterProiView protein in InterPro
IPR000907 LipOase
IPR013819 LipOase_C
IPR036226 LipOase_C_sf
IPR020834 LipOase_CS
IPR020833 LipOase_Fe_BS
IPR001885 LipOase_mml
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
PANTHERiPTHR11771 PTHR11771, 1 hit
PfamiView protein in Pfam
PF00305 Lipoxygenase, 1 hit
PF01477 PLAT, 1 hit
PRINTSiPR00087 LIPOXYGENASE
PR00467 MAMLPOXGNASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF48484 SSF48484, 1 hit
SSF49723 SSF49723, 1 hit
PROSITEiView protein in PROSITE
PS00711 LIPOXYGENASE_1, 1 hit
PS00081 LIPOXYGENASE_2, 1 hit
PS51393 LIPOXYGENASE_3, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

F1LQ70-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRYRVRVVT GAWLFSGSVN LVRLWLVGAH REARLELQLR PARGKEEEFD
60 70 80 90 100
FDVAEDLGPL QFVKLHKEHT VVDDAWFCNL ITVQGPGMSA EAVFPCYRWV
110 120 130 140 150
QGEGILRLPE GTARLAGDNA LDVFQKHREK ELKERQQTYR WATWKQGLPQ
160 170 180 190 200
TIAADCKDDL PPNMRFHEEK RLDFEWTLKA GVLEMGLKRV YTLLRSWNRL
210 220 230 240 250
EDFDQIFWGQ KSALAEKVHR CWQEDELFGY QFLNGANPML LRRSTSLPSR
260 270 280 290 300
LVLPPGTEEL QAQLEKELKD GCLFEADFIL LDGIPANVIR GEQQYLAAPL
310 320 330 340 350
VMLRMDPSGK LLPMAIQIQP PNPSSPAPTL FLPSDPPLAW LLAKIWVRNS
360 370 380 390 400
DFQLQELQFH LLNTHLVAEV IAVATMRCLP GLHPIFKLLV PHIRYTMEIN
410 420 430 440 450
TRSRTQLISD GGIFDQVVST GGGGHVQLLT RAVAQLTYCS LCPPDDLATR
460 470 480 490 500
GLLRVPSALY AQDALQLWEV TARYVNGMVH LFYQSDDIVR GDPELQAWCR
510 520 530 540 550
EITEVGLCHA QDRGFPVSFQ SRAQLCHFLT MCVFTCTSQH AAINQGQLDW
560 570 580 590 600
YGWVPNAPCT MRMPPPTSKD DVTMETIMGS LPDVQKSCLQ MTITWHLGRL
610 620 630 640 650
QPDMVPLGHH KEKYFSDPRT KAVLSQFQAD LENLEKEITA RNQQLDLPYE
660
YLKPSRIENS ITI
Length:663
Mass (Da):75,534
Last modified:May 3, 2011 - v1
Checksum:i2DC11842CDA025CA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR06064404 Genomic DNA No translation available.
UniGeneiRn.66513

Genome annotation databases

EnsembliENSRNOT00000033098; ENSRNOP00000034009; ENSRNOG00000027037
UCSCiRGD:1311159 rat

Similar proteinsi

Entry informationi

Entry nameiLOX12_RAT
AccessioniPrimary (citable) accession number: F1LQ70
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
Last sequence update: May 3, 2011
Last modified: May 23, 2018
This is version 51 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
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